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    Synthesis and characterization of a chitosan/gelatin transparent film crosslinked with a combination of EDC/NHS for corneal epithelial cell culture scaffold with potential application in cornea implantation

    , Article International Journal of Polymeric Materials and Polymeric Biomaterials ; Volume 71, Issue 8 , 2022 , Pages 568-578 ; 00914037 (ISSN) Shahin, A ; Ramazani S. A, A ; Mehraji, S ; Eslami, H ; Sharif University of Technology
    Taylor and Francis Ltd  2022
    Abstract
    The diseases and disorders of the cornea may lead to blindness, and cornea transplantation has been an effective treatment in this regard. However, lack of cornea throughout the world makes this treatment difficult. Therefore, the first goal of the present study is to make a chitosan/gelatin hyaline film with NHS and EDC crosslinkers for transplanting the epithelial cells of the cornea. Two solutions of gelatin and chitosan were mixed homogeneously before crosslinking in ratios of 20/80, 30/70, 40/60, and 50/50 (Gel/Chi). After 24 hours, they were put in an oven to dry, then EDC and NHS were added to the mixture as crosslinker. Corneal epithelial cell morphology was assessed qualitatively... 

    Cytotoxicity of protein corona-graphene oxide nanoribbons on human epithelial cells

    , Article Applied Surface Science ; Volume 320 , 30 November , 2014 , Pages 596-601 ; ISSN: 01694332 Mbeh, D. A ; Akhavan, O ; Javanbakht, T ; Mahmoudi, M ; Yahia, L ; Sharif University of Technology
    Abstract
    Graphene oxide nanoribbons (GONRs) were synthesized using an oxidative unzipping of multi-walled carbon nanotubes. The interactions of the GONRs with various concentrations of fetal bovine serum or human plasma serum indicated that the GONRs were functionalized substantially by the albumin originated from the two different protein sources. Then, concentration-dependent cytotoxicity of the protein-functionalized GONRs on human epithelial cells was studied. Although the GONRs with concentrations ≤50 μg/mL did not exhibit significant cytotoxicity on the cells (with the cell viability >85%), the concentration of 100 μg/mL exhibited significant cytotoxicity including prevention of cell... 

    Carbon nanotubes-graft-polyglycerol: biocompatible hybrid materials for nanomedicine

    , Article Polymer ; Volume 50, Issue 15 , 2009 , Pages 3528-3536 ; 00323861 (ISSN) Adeli, M ; Mirab, N ; Shafiee Alavidjeh, M ; Sobhani, Z ; Atyabi, F ; Sharif University of Technology
    2009
    Abstract
    New biocompatible and water soluble hybrid materials containing multi-wall carbon nanotubes (MWCNTs) as core and hyperbranched polyglycerol (PG) as shell were synthesized successfully. In this work, pristine MWCNTs were opened and functionalized through treatment with acid and polyglycerol was covalently grafted onto their surface by the "grafting from" approach based on in-situ ring-opening polymerization of glycidol. Some short-term In vitro cytotoxicity and hemocompatibility tests were conducted on HT1080 cell line (human Fibrosarcoma), because this epithelial cell line can be one of the first route of entry of the exogenous materials to the vascular system and therefore subsequent... 

    Recent advancements in aptamer-bioconjugates: Sharpening stones for breast and prostate cancers targeting

    , Article Journal of Drug Delivery Science and Technology ; Volume 53 , 2019 ; 17732247 (ISSN) Maghsoudi, S ; Shahraki, B. T ; Rabiee, N ; Afshari, R ; Fatahi, Y ; Dinarvand, R ; Ahmadi, S ; Bagherzadeh, M ; Rabiee, M ; Tahriri, M ; Tayebi, L ; Sharif University of Technology
    Editions de Sante  2019
    Abstract
    Breast and prostate cancers are common types of cancers with various strategies, such as chemotherapy and radiotherapy, for their therapy. Since these methods have undesired side effects and poor target affinity, neoteric strategies—known as aptamer-based smart drug delivery systems (SDDSs)—have been developed in recent years to overcome the obstacles of current treatment, and investigated for a clinical trial. The high affinity and versatility of aptamers for binding to the corresponding targets make them highly noticeable agents in the drug delivery domains. In addition to their exceptional benefits, aptamers are able to overcome tumor resistance because of their high selectivity and low... 

    Smart liposomal drug delivery for treatment of oxidative stress model in human embryonic stem cell-derived retinal pigment epithelial cells

    , Article International Journal of Pharmaceutics ; Volume 548, Issue 1 , 2018 , Pages 62-72 ; 03785173 (ISSN) Behroozi, F ; Abdkhodaie, M. J ; Sadeghi Abandansari, H ; Satarian, L ; Kazemi Ashtiani, M ; Jaafari, M. R ; Baharvand, H ; Sharif University of Technology
    Abstract
    Oxidative stress has been implicated in the progression of age-related macular degeneration (AMD). Treatment with antioxidants seems to delay progression of AMD. In this study, we suggested an antioxidant delivery system based on redox-sensitive liposome composed of phospholipids and a diselenide centered alkyl chain. Dynamic light scattering assessment indicated that the liposomes had an average size of 140 nm with a polydispersity index below 0.2. The percentage of encapsulation efficiency of the liposomes was calculated by high-performance liquid chromatography. The carriers were loaded with N-acetyl cysteine as a model antioxidant drug. We demonstrated responsiveness of the nanocarrier... 

    Study of epithelial differentiation and protein expression of keratinocyte-mesenchyme stem cell co-cultivation on electrospun nylon/B. vulgaris extract composite scaffold

    , Article Materials Science and Engineering C ; Volume 75 , 2017 , Pages 653-662 ; 09284931 (ISSN) Hosseinzadeh, S ; Soleimani, M ; Vossoughi, M ; Ranjbarvan, P ; Hamedi, S ; Zamanlui, S ; Mahmoudifard, M ; Sharif University of Technology
    Abstract
    Employing of the composite electrospun scaffold containing herbal extract in conjugation with co-culturing of cells can open up new window to the design of efficient biomaterials for skin tissue regeneration. Here, we introduce the synergistic effect of composite electrospun nanofibrous scaffold of nylon66 loaded with Beta vulgaris (B. vulgaris) (extract of beet roots, a plants whose widely used in Iranian folk medicine as wound healing medicine) and co-culture of mesenchymal stem-cells (MSCs)-human keratinocyte (H-keratino) differentiation towards epithelial lineage. In vitro biocompatibility was examined through MTT assay and epithelial differentiation checked by real-time PCR and... 

    Aptamer hybrid nanocomplexes as targeting components for antibiotic/gene delivery systems and diagnostics: a review

    , Article International Journal of Nanomedicine ; Volume 15 , 2020 , Pages 4237-4256 Ahmadi, S ; Arab, Z ; Safarkhani, M ; Nasseri, B ; Rabiee, M ; Tahriri, M ; Webster, T. J ; Tayebi, L ; Rabiee, N ; Sharif University of Technology
    Dove Medical Press Ltd  2020
    Abstract
    With the passage of time and more advanced societies, there is a greater emergence and incidence of disease and necessity for improved treatments. In this respect, nowadays, aptamers, with their better efficiency at diagnosing and treating diseases than antibodies, are at the center of attention. Here, in this review, we first investigate aptamer function in various fields (such as the detection and remedy of pathogens, modification of nanoparticles, antibiotic delivery and gene delivery). Then, we present aptamer-conjugated nanocomplexes as the main and efficient factor in gene delivery. Finally, we focus on the targeted co-delivery of genes and drugs by nanocomplexes, as a new exciting... 

    Unraveling cancer metastatic cascade using microfluidics-based technologies

    , Article Biophysical Reviews ; Volume 14, Issue 2 , 2022 , Pages 517-543 ; 18672450 (ISSN) Hakim, M ; Kermanshah, L ; Abouali, H ; Hashemi, H. M ; Yari, A ; Khorasheh, F ; Alemzadeh, I ; Vossoughi, M ; Sharif University of Technology
    Springer Science and Business Media Deutschland GmbH  2022
    Abstract
    Cancer has long been a leading cause of death. The primary tumor, however, is not the main cause of death in more than 90% of cases. It is the complex process of metastasis that makes cancer deadly. The invasion metastasis cascade is the multi-step biological process of cancer cell dissemination to distant organ sites and adaptation to the new microenvironment site. Unraveling the metastasis process can provide great insight into cancer death prevention or even treatment. Microfluidics is a promising platform, that provides a wide range of applications in metastasis-related investigations. Cell culture microfluidic technologies for in vitro modeling of cancer tissues with fluid flow and the...