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Liquid-liquid-liquid microextraction and high performance liquid Chromatography for Seperation and Determination of Estrogens in Aquatic Media
,
M.Sc. Thesis
Sharif University of Technology
;
Bagheri, Habib
(Supervisor)
Abstract
Liquid-liquid-liquid microextraction (LLLME) is a new miniaturized liquid extraction to preconcentrate and purify for ionizable analytes. In LLLME firstly analytes extracted in an organic phase, and subsequently back-extracted into a second aqueous phase. Main advantages of LLME are their speed, simplicity, negligible volume of solvents, analytical precision, short sample preparation and low cost. This technique was shown to be an efficient preconcentration method for the extraction of estrone and estriol from biological samples. A basic microdrop in the tip of microsyringe was fixed in the organic layer over the sample solution. After extraction for a preset time, the microdrop was...
Electrospun Functionalized Silica / Polyvinyl Alcohol Nanofibers for On-Line μ-Solid Phase Extraction of Estrone and Estradiol in Biological Samples
, M.Sc. Thesis Sharif University of Technology ; Bagheri, Habib (Supervisor)
Abstract
A method for determination of estrogens in biological samples was developed. This method was based on high performance liquid chromatography using online micro-solid phase extraction with 25 mg sorbent synthesized by electrospining of silica/PVA nanofibers. Poly (vinyl alcohol) (PVA) is an easily electrospun polymer with unique properties such as biocompatibility, processability, and hydrophilicity. Silica is biocompatible, thermally stable, and comparatively inert. Incorporation of silica with PVA produces hybrids with enhanced thermal and chemical stability in sample media and elution solvents without relinquishing the biocompatibility of PVA. The sufficient numbers of disc sheets were cut...
Application of glassy carbon electrode modified with a carbon nanoparticle/melamine thin film for voltammetric determination of raloxifene
, Article Journal of Electroanalytical Chemistry ; Volume 780 , 2016 , Pages 126-133 ; 15726657 (ISSN) ; Ghalkhani, M ; Balotf, H ; Salimian, R ; Sharif University of Technology
Elsevier B.V
2016
Abstract
As a selective estrogen receptor modulator, raloxifene (RXF) prevent of osteoporosis in postmenopausal women by estrogenic actions on bone and decreases the risk of invasive breast cancer by anti-estrogenic actions on the breast and uterus tissue. However, RXF may increase the risk of blood clots, including deep vein thrombosis and pulmonary embolism. For the first time glassy carbon electrode modified with a thin film of melamine/carbon nanoparticles (CNPs/Mela) was constructed and used for the sensitive voltammetric determination of RXF. In comparison with unmodified electrode, the presence of the CNPs/Mela film resulted in a remarkable increase in the peak currents and sharpness of the...
Opposite trends of GAS6 and GAS6-AS expressions in breast cancer tissues
, Article Experimental and Molecular Pathology ; Volume 118 , 2021 ; 00144800 (ISSN) ; Hussen, B. M ; Taheri, F ; Sattari, A ; Yousefi, H ; Omrani, M. D ; Nazer, N ; Ghafouri Fard, S ; Taheri, M ; Sharif University of Technology
Academic Press Inc
2021
Abstract
Growth arrest-specific gene 6 (GAS6) is a growth factor-like cytokine whose function is related with vitamin K. This protein interacts with receptor tyrosine kinase proteins such as Tyro3, Axl, and TAM Receptor family, therefore affecting the tumorigenic processes via different mechanisms. GAS6-antisense 1 (GAS6-AS1) is a long non-coding RNAs (lncRNAs) that is transcribed from a genomic regions nearby GAS6. This lncRNA is also implicated in the pathobiology of cancer. We intended to judge the role of GAS6 and GAS6-AS1 in the pathogenesis of breast cancer through appraisal of their expression levels in breast cancer tissues and their paired neighboring non-cancerous samples. Expression of...
Cytochrome 4Z1 expression is associated with unfavorable survival in triple-negative breast cancers
, Article Breast Cancer: Targets and Therapy ; Volume 13 , 2021 , Pages 565-574 ; 11791314 (ISSN) ; Alshammari, F. O. F. O ; Youssef, A. M. M ; Al Tarawneh, F ; Al Sarayreh, S ; Almuhaisen, G. H ; Satari, A. O ; Al Shuneigat, J ; Alrawashdeh, H. M ; Sharif University of Technology
Dove Medical Press Ltd
2021
Abstract
Purpose: Triple-negative breast cancer (TNBC) is characterized by high mortality rate, and its clinical management is difficult and complex. Therefore, there is a need for extensive efforts aimed at accelerating the discovery of novel therapies for TNBC. CYP4Z1 has been implicated in the development of breast cancer. The current study aimed at characterizing the expression of CYP4Z1 on TNBC. Materials and Methods: Using immunohistochemistry, CYP4Z1 expression was evaluated on 122 TNBC samples, four samples of breast cancers expressing ER, PR, and HER-2, and four samples of normal breast tissues. The association between the enzyme and various histopathological features and survival of...
Glypican-1 overexpression in different types of breast cancers
, Article OncoTargets and Therapy ; Volume 14 , 2021 , Pages 4309-4318 ; 11786930 (ISSN) ; Al Saraireh, Y. M ; Youssef, A. M. M ; Al Sarayra, Y. M ; Alrawashdeh, H. M ; Sharif University of Technology
Dove Medical Press Ltd
2021
Abstract
Purpose: Treatment of metastatic breast cancer patients is challenging and remains a major underlying cause of female mortality. Understanding molecular alterations in tumor development is critical to identify novel biomarkers and targets for cancer diagnosis and therapy. One of the aberrant cancer expressions gaining recent research interest is glypican-1. Several studies reported strong glypican-1 expression in various types of human cancers. However, none of these investigated glypican-1 expression in a large cohort of breast cancer histopathological subtypes. Patients and Methods: Immunohistochemistry was used to assess glypican-1 expression in 220 breast cancer patients and its relation...
Role of precursors and coating polymers in sol-gel chemistry toward enhanced selectivity and efficiency in solid phase microextraction
, Article Analytica Chimica Acta ; Volume 742 , 2012 , Pages 45-53 ; 00032670 (ISSN) ; Piri Moghadam, H ; Ahdi, T ; Sharif University of Technology
Elsevier
2012
Abstract
To evaluate the selectivity and efficiency of solid phase microextraction (SPME) fiber coatings, synthesized by sol-gel technology, roles of precursors and coating polymers were extensively investigated. An on-line combination of capillary microextraction (CME) technique and high performance liquid chromatography (HPLC) was set up to perform the investigation. Ten different fiber coatings were synthesized in which five of them contained only the precursor and the rests were prepared using both the precursor and coating polymer. All the coatings were chemically bonded to the inner surface of copper tubes, intended to be used as the CME device and already functionalized by self-assembly...
Delivery of melittin-loaded niosomes for breast cancer treatment: an in vitro and in vivo evaluation of anti-cancer effect
, Article Cancer Nanotechnology ; Volume 12, Issue 1 , 2021 ; 18686958 (ISSN) ; Akbarzadeh, I ; Marzbankia, E ; Farid, M ; khaledi, L ; Reihani, A. H ; Javidfar, M ; Mortazavi, P ; Sharif University of Technology
BioMed Central Ltd
2021
Abstract
Background: Melittin, a peptide component of honey bee venom, is an appealing candidate for cancer therapy. In the current study, melittin, melittin-loaded niosome, and empty niosome had been optimized and the anticancer effect assessed in vitro on 4T1 and SKBR3 breast cell lines and in vivo on BALB/C inbred mice. "Thin-layer hydration method" was used for preparing the niosomes; different niosomal formulations of melittin were prepared and characterized in terms of morphology, size, polydispersity index, encapsulation efficiency, release kinetics, and stability. A niosome was formulated and loaded with melittin as a promising drug carrier system for chemotherapy of the breast cancer cells....