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fluorescein-isothiocyanate
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Highly sensitive turn-on fluorescent detection of captopril based on energy transfer between fluorescein isothiocyanate and gold nanoparticles
, Article Journal of Luminescence ; Volume 134 , 2013 , Pages 874-879 ; 00222313 (ISSN) ; Bagheri, H ; Bohloul, A ; Taheri, N ; Robatjazi, H ; Sharif University of Technology
2013
Abstract
A novel approach for highly sensitive detection of captopril was developed based on the fluorescence resonance energy transfer (FRET) between gold nanoparticles (Au NPs) and fluorescein isothiocyanate (FITC), in which FITC acts as the donor and Au NPs as the acceptor. The fluorescence intensity of fluorescein isothiocyanate (FITC) was strictly quenched as a result of noncovalently adsorbed on Au NPs. Upon the addition of captopril, the fluorescence intensity of FITC turn-on due to the competition between captopril and FITC towards the surface of Au NPs. Under the optimum conditions, the fluorescence intensity of the released FITC displays a linear relationship in the range of 20 μg L-1 to...
An ultrasensitive and selective turn-off fluorescent nanoprobe for the detection of copper ions
, Article Analytical Methods ; Volume 7, Issue 12 , 2015 , Pages 5067-5073 ; 17599660 (ISSN) ; Taghipour, M ; Sharif University of Technology
Royal Society of Chemistry
2015
Abstract
In this study, a novel approach for sensitive and extremely selective detection of copper ions has been developed based on fluorescence resonance energy transfer (FRET) between AuNPs as an acceptor and fluorescein isothiocyanate (FITC) as a donor. Initially, the fluorescence of the FITC molecule turns-off due to adsorption to the AuNPs surface. However, the fluorescence of the FITC-AuNPs system was switched to turn-on by adding d-Pencillamine (d-PC) to the solution mixtures as FITC molecules are released upon displacement by d-PC on the NPs surface. The higher affinity of d-PC toward the surface of AuNPs was further evidenced by controlling the fluorescence intensity enhancement of the...
Development of a nano biosensor for anti-gliadin detection for Celiac disease based on suspension microarrays
, Article Biomedical Physics and Engineering Express ; Volume 6, Issue 5 , August , 2020 ; Rabiee, M ; Rostami Nejad, M ; Aghamohammadi, E ; Asadzadeh Aghdaei, H ; Zali, M. R ; Rabiee, N ; Fatahi, Y ; Bagherzadeh, M ; Webster, T. J ; Sharif University of Technology
IOP Publishing Ltd
2020
Abstract
Celiac disease is an autoimmune disorder represented by the ingestion of the gluten protein usually found in wheat, barley and rye. To date, ELISA has been the most accurate method for determining the presence of anti-gliadin, which is cumbersome, expensive (compared to a suspension microarray technique), and requires extensive sample preparation. In this study, in order to establish a more accurate assay to identify gliadin at lower concentrations, optical nano biosensors using an indirect immunoassay method for gliadin detection was designed and fabricated. For this, polycaprolactone (PCL) nano- to micro-beads were fabricated as a platform for the gliadin antigen which were optimized and...