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Article Industrial and Engineering Chemistry Research ; Volume 48, Issue 23 , Volume 48, Issue 23 , 2009 , Pages 10614-10618 ; 08885885 (ISSN) ; Ghaemmaghami, F ; Alemzadeh, I ; Sharif University of Technology
Partial purification of plant peroxidase from turnip (Brassica rapa) was optimized. Aqueous two phase system and precipitation by ammonium sulfate (as two parallel purification methods) were used. Polyethylene glycol/ammonium sulfate/NaCl (25:7:3%, w/v) system followed by gel-filtration chromatography gave a purification factor of about 36 fold. On the other hand, ammonium sulfate precipitation (60-80% deg) followed by gel filtration gave only about 13 purification fold. Hence, the aqueous two-phase system was more efficient and useful method as a primary purification step since it was less laborious, less time-consuming, and led to more purification factor. The partially purified turnip...