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gene-delivery
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Viral and non-viral gene therapy using 3D (bio)printing
, Article Journal of Gene Medicine ; Volume 24, Issue 12 , 2022 ; 1099498X (ISSN) ; Rahimnejad, M ; Nasrollahi Boroujeni, N ; Ahmadi, Z ; Motamed Fath, P ; Ahmadi, S ; Safarkhani, M ; Rabiee, N ; Sharif University of Technology
John Wiley and Sons Inc
2022
Abstract
The overall success in launching discovered drugs is tightly restricted to the high rate of late-stage failures, which ultimately inhibits the distribution of medicines in markets. As a result, it is imperative that methods reliably predict the effectiveness and, more critically, the toxicity of medicine early in the drug development process before clinical trials be continuously innovated. We must stay up to date with the fast appearance of new infections and diseases by rapidly developing the requisite vaccinations and medicines. Modern in vitro models of disease may be used as an alternative to traditional disease models, and advanced technology can be used for the creation of...
Photoluminescent carbon quantum dot/poly-L-Lysine core-shell nanoparticles: A novel candidate for gene delivery
, Article Journal of Drug Delivery Science and Technology ; 2020 ; Mofazzal Jahromi, M. A ; Abdoli, A ; Mohammad Beigi, H ; Fatahi, Y ; Nourizadeh, H ; Zare, H ; Kiani, J ; Radmanesh, F ; Rabiee, N ; Jahani, M ; Mombeiny, R ; Karimi, M ; Sharif University of Technology
Editions de Sante
2020
Abstract
Cationic polymers such as poly-L-lysine (PLL) are able to interact electrostatically with DNA to produce polymeric systems with nanometric diameters due to the neutralization and accumulation of DNA. This study integrates the outstanding properties of carbon quantum dots (CQDs) with PLL to develop a novel gene delivery vehicle with a core-shell hybrid nanostructure. The CQD/PLL core-shell nanoparticles (NPs) were, therefore, synthesized in such a way that they had narrow size distribution and an average diameter under 10 nm, both of which were confirmed by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Fourier transform infrared (FTIR) spectroscopy exhibited that...
Synthesis and Characterization of Zeolitic Imidazolate Framework-8 as a Carrier for Gene Therapy Applications
, M.Sc. Thesis Sharif University of Technology ; Simchi, Abdolreza (Supervisor) ; Behmanesh, Mehrdad (Co-Supervisor)
Abstract
Advances in genetics and biology have led to the ability to produce therapeutic genes and modify defective genes in patients' genomes, resulting in a solution to many genetic diseases. Many genetic diseases can be overcome through gene therapy by transferring therapeutic genes into the cell nucleus. However, researchers have not yet been able to develop a safe, high-efficiency carrier for the transfer of genes to the target cell, and this has become a bottleneck for the clinical applications of gene therapy. In this study, the ZIF-8 organic-metallic framework was used as a new vector for the transfer of therapeutic genes to target cells. For this purpose, ZIF-8 was synthesized by a...
Smart micro/nano-robotic systems for gene delivery
, Article Current Gene Therapy ; Volume 17, Issue 2 , 2017 , Pages 73-79 ; 15665232 (ISSN) ; Nejat Pishkenari, H ; Sharif University of Technology
Bentham Science Publishers B.V
2017
Abstract
Background: Small scale robotics have attracted growing attention for the prospect of targeting and accessing cell-sized sites, necessary for high precision biomedical applications and drug/gene delivery. The loss of controlled gene therapy, inducing systemic side effects and reduced therapeutic efficiency, can be settled utilizing these intelligent carriers. Methods: Newly proposed solutions for the main challenges of control, power supplying, gene release and final carrier extraction/degradation have shifted these smart miniature robots to the point of being employed for practical applications of transferring oligonucleotides (pDNA, siRNA, mRNA, etc.) in near future. Conclusion: In this...
Applications of graphene and graphene oxide in smart drug/gene delivery: Is the world still flat?
, Article International Journal of Nanomedicine ; Volume 15 , 2020 , Pages 9469-9496 ; Mirkiani, S ; Mozaffari, N ; Abdolahi Sadatlu, M. A ; Ghasemi, A ; Abbaspour, S ; Akbarian, M ; Farjadian, F ; Karimi, M ; Sharif University of Technology
Dove Medical Press Ltd
2020
Abstract
Graphene, a wonder material, has made far-reaching developments in many different fields such as materials science, electronics, condensed physics, quantum physics, energy systems, etc. Since its discovery in 2004, extensive studies have been done for understanding its physical and chemical properties. Owing to its unique characteristics, it has rapidly became a potential candidate for nano-bio researchers to explore its usage in biomedical applications. In the last decade, remarkable efforts have been devoted to investigating the biomedical utilization of graphene and graphene-based materials, especially in smart drug and gene delivery as well as cancer therapy. Inspired by a great number...
Temperature-responsive smart nanocarriers for delivery of therapeutic agents: applications and recent advances
, Article ACS Applied Materials and Interfaces ; Volume 8, Issue 33 , 2016 , Pages 21107-21133 ; 19448244 (ISSN) ; Sahandi Zangabad, P ; Ghasemi, A ; Amiri, M ; Bahrami, M ; Malekzad, H ; Ghahramanzadeh Asl, H ; Mahdieh, Z ; Bozorgomid, M ; Ghasemi, A ; Rahmani Taji Boyuk, M. R ; Hamblin, M. R ; Sharif University of Technology
American Chemical Society
2016
Abstract
Smart drug delivery systems (DDSs) have attracted the attention of many scientists, as carriers that can be stimulated by changes in environmental parameters such as temperature, pH, light, electromagnetic fields, mechanical forces, etc. These smart nanocarriers can release their cargo on demand when their target is reached and the stimulus is applied. Using the techniques of nanotechnology, these nanocarriers can be tailored to be target-specific, and exhibit delayed or controlled release of drugs. Temperature-responsive nanocarriers are one of most important groups of smart nanoparticles (NPs) that have been investigated during the past decades. Temperature can either act as an external...
Carbosilane dendrimers: Drug and gene delivery applications
, Article Journal of Drug Delivery Science and Technology ; Volume 59 , 2020 ; Ahmadvand, S ; Ahmadi, S ; Fatahi, Y ; Dinarvand, R ; Bagherzadeh, M ; Rabiee, M ; Tahriri, M ; Tayebi, L ; Hamblin, M. R ; Sharif University of Technology
Editions de Sante
2020
Abstract
Carbosilane dendrimers are a particular type of dendrimer structure that has been used as delivery vehicles for drugs and nucleic acids. They have a defined structure according to their generation number, and their terminal groups can be rendered cationic or anionic. The cationic charges can address the limitation of electrostatic repulsion between the negatively charged phosphate groups of nucleic acids and negatively charged cell membranes. Specific drugs can be loaded into the central part of the dendrimer or attached at the exterior, and the overall positive charge may improve the efficacy of anti-inflammatory drugs. One promising feature of dendrimers is their non-toxicity both in vitro...
Synthesis, characterization and mechanistic study of nano chitosan tetrazole as a novel and promising platform for CRISPR delivery
, Article International Journal of Polymeric Materials and Polymeric Biomaterials ; 2020 ; Bagherzadeh, M ; Tavakolizadeh, M ; Pourjavadi, A ; Atarod, M ; Webster, T. J ; Sharif University of Technology
Taylor and Francis Inc
2020
Abstract
Here, a newly modified chitosan tetrazole was synthesized and fully characterized for CRISPR technology since by increasing the ratio of nitrogen to other elements, the interaction between the genetic material and this nanomaterial was significantly enhanced. The nanomaterial was added to HEK-293 cells for pCRISPR delivery. The nanomaterials were optimized and showed an acceptable binding ability with DNA due to its increasing zeta potential in the synthesized chitosan tetrazole in comparison with chitosan, as well as considerable transfection efficiency, more than 25%, and, thus, can be considered as a promising and also new gold-standard for the delivery of pCRISPR. © 2020 Taylor & Francis...
Synthesis, characterization and mechanistic study of nano chitosan tetrazole as a novel and promising platform for CRISPR delivery
, Article International Journal of Polymeric Materials and Polymeric Biomaterials ; Volume 71, Issue 2 , 2022 , Pages 116-126 ; 00914037 (ISSN) ; Bagherzadeh, M ; Tavakolizadeh, M ; Pourjavadi, A ; Atarod, M ; Webster, T. J ; Sharif University of Technology
Taylor and Francis Ltd
2022
Abstract
Here, a newly modified chitosan tetrazole was synthesized and fully characterized for CRISPR technology since by increasing the ratio of nitrogen to other elements, the interaction between the genetic material and this nanomaterial was significantly enhanced. The nanomaterial was added to HEK-293 cells for pCRISPR delivery. The nanomaterials were optimized and showed an acceptable binding ability with DNA due to its increasing zeta potential in the synthesized chitosan tetrazole in comparison with chitosan, as well as considerable transfection efficiency, more than 25%, and, thus, can be considered as a promising and also new gold-standard for the delivery of pCRISPR. © 2020 Taylor & Francis...
Carbon nanotubes part II: A remarkable carrier for drug and gene delivery
, Article Expert Opinion on Drug Delivery ; Volume 12, Issue 7 , 2015 , Pages 1089-1105 ; 17425247 (ISSN) ; Solati, N ; Ghasemi, A ; Estiar, M. A ; Hashemkhani, M ; Kiani, P ; Mohamed, E ; Saeidi, A ; Taheri, M ; Avci, P ; Aref, A. R ; Amiri, M ; Baniasadi, F ; Hamblin, M. R ; Sharif University of Technology
Informa Healthcare
2015
Abstract
Introduction: Carbon nanotubes (CNT) have recently been studied as novel and versatile drug and gene delivery vehicles. When CNT are suitably functionalized, they can interact with various cell types and are taken up by endocytosis.Areas covered: Anti-cancer drugs cisplatin and doxorubicin have been delivered by CNT, as well as methotrexate, taxol and gemcitabine. The delivery of the antifungal compound amphotericin B and the oral administration of erythropoietin have both been assisted using CNT. Frequently, targeting moieties such as folic acid, epidermal growth factor or various antibodies are attached to the CNT-drug nanovehicle. Different kinds of functionalization (e.g., polycations)...
Synthesis, Characterization and Application of Inorganic Nanosystems based on Biocompatible and Highly Tunable Materials for Targeted Delivery and Release of Gene and Drug
, Ph.D. Dissertation Sharif University of Technology ; Bagherzadeh, Mojtaba (Supervisor)
Abstract
In this dissertation, an attempt has been made to prepare a library from most of the inorganic and organic compounds in order to strengthen the use of nanosystems for the purpose of controlled delivery and release of drugs and genetic material. In this regard, a complete set of compounds based on metal nanoparticles, layered double hydroxides, metal-organic frameworks, graphene oxide and carbon nanotubes were designed, synthesized and characterized, and nanocomposites and hybrids of them based on palladium, zinc, copper and nickel were designed as well. The nano-stable system is designed with positive surface potential, very low cytotoxicity, biodegradability and acceptable environment as...
Poly-L-lysine/hyaluronan nanocarriers as a novel nanosystem for gene delivery
, Article Journal of Microscopy ; Volume 287, Issue 1 , 2022 , Pages 32-44 ; 00222720 (ISSN) ; Bagherzadeh, M. A ; Mofazzal Jahromi, M. A ; Mohammad-Beigi, H ; Abdoli, A ; Mir, H ; Roustazadeh, A ; Pirestani, M ; Sahandi Zangabad, P ; Kiani, J ; Bakhshayesh, A ; Jahani, M ; Joghataei, M. T ; Karimi, M ; Sharif University of Technology
John Wiley and Sons Inc
2022
Abstract
The present research comes up with a novel DNA-loaded poly-L-lysine (PLL)/hyaluronan (HA) nanocarrier (DNA-loaded PLL/HA NCs) for gene delivery applications, as a promising candidate for gene delivery into diverse cells. A straightforward approach was employed to prepare such a nanosystem through masking DNA-loaded PLL molecules by HA. Fourier-transform infrared (FTIR) spectroscopy, dynamic light scattering (DLS), field emission-scanning electron microscopy (FE-SEM) and transmission electron microscopy (TEM) were used to analyse the interaction of the molecules as well as the physicochemical properties of the NCs. The NCs showed a negative charge of –24 ± 3 mV, with an average size of 138 ±...
Histidine-enhanced gene delivery systems: The state of the art
, Article Journal of Gene Medicine ; Volume 24, Issue 5 , 2022 ; 1099498X (ISSN) ; Jahanpeimay Sabet, M ; Hasanzadeh, A ; Kamrani Mousavi, S. M ; Haeri Moghaddam, N ; Hooshmand, S. A ; Rabiee, N ; Liu, Y ; Hamblin, M. R ; Karimi, M ; Sharif University of Technology
John Wiley and Sons Inc
2022
Abstract
Gene therapy has emerged as a promising tool for treating different intractable diseases, particularly cancer or even viral diseases such as COVID-19 (coronavirus disease 2019). In this context, various non-viral gene carriers are being explored to transfer DNA or RNA sequences into target cells. Here, we review the applications of the naturally occurring amino acid histidine in the delivery of nucleic acids into cells. The biocompatibility of histidine-enhanced gene delivery systems has encouraged their wider use in gene therapy. Histidine-based gene carriers can involve the modification of peptides, dendrimers, lipids or nanocomposites. Several linear polymers, such as polyethylenimine,...
Bottom-up synthesis of nitrogen and oxygen co-decorated carbon quantum dots with enhanced DNA plasmid expression
, Article Colloids and Surfaces B: Biointerfaces ; Volume 184 , 2019 ; 09277765 (ISSN) ; Khezri, J ; Esfandiari, S ; Mahdavi, H ; Karkhane, A. A ; Rahighi, R ; Heidarimoghadam, R ; Tayebi, L ; Hashemi, E ; Farmany, A ; Sharif University of Technology
Elsevier B.V
2019
Abstract
In this paper, a bottom-up hydrothermal route is reported for the synthesis of oxygen and nitrogen co-decorated carbon quantum dots (CQDs) using ammonium hydrogen citrate (AHC) as a single precursor. DLS data approved the formation of 4.0 nm (average size) CQDs. XRD pattern shows the interlayer spacing (002) of 3.5 Å for CQDs, which is exactly the same as that of crystalline graphite. XPS and FTIR spectra verified the formation of oxygen and nitrogen functional groups on the CQDs surface. Co-decorated carboxyl, hydroxyl and amine groups on the CQDs surfaces make them as promising polyelectrolyte for gene delivery. Toxicity assay showed a survival rate of 70% under different incubation times...
Superparamagnetic iron oxide nanoparticles with rigid cross-linked polyethylene glycol fumarate coating for application in imaging and drug delivery
, Article Journal of Physical Chemistry C ; Volume 113, Issue 19 , 2009 , Pages 8124-8131 ; 19327447 (ISSN) ; Simchi, A ; Imani, M ; Hafeli, U. O ; Sharif University of Technology
2009
Abstract
Superparamagnetic iron oxide nanoparticles with proper surface coatings are increasingly being evaluated for clinical applications such as hyperthermia, drug delivery, magnetic resonance imaging, transfection, and cell/protein separation. To enhance the applicability of magnetic nanoparticles, two main problems must be overcome. First, as the drug coats the particle surface, a significant portion of it is quickly released upon injection (burst effect). Therefore, only small amounts of the drug reach the specific site after, for example, magnetic drug targeting. Second, once the surface-derivatized nanoparticles are inside the cells, the coating is likely digested, leaving the bare particles...
Diatoms with invaluable applications in nanotechnology, biotechnology, and biomedicine: Recent advances
, Article ACS Biomaterials Science and Engineering ; Volume 7, Issue 7 , 2021 , Pages 3053-3068 ; 23739878 (ISSN) ; Khatami, M ; Jamalipour Soufi, G ; Fatahi, Y ; Iravani, S ; Varma, R. S ; Sharif University of Technology
American Chemical Society
2021
Abstract
Diatoms are unicellular microalga found in soil and almost every aquatic environment (marine and fresh water). Biogenic silica and diatoms are attractive for biotechnological and industrial applications, especially in the field of biomedicine, industrial/synthetic manufacturing processes, and biomedical/pharmaceutical sciences. Deposition of silica by diatoms allows them to create micro- or nanoscale structures which may be utilized in nanomedicine and especially in drug/gene delivery. Diatoms with their unique architectures, good thermal stability, suitable surface area, simple chemical functionalization/modification procedures, ease of genetic manipulations, optical/photonic...
Photoluminescent carbon quantum dot/poly-L-Lysine core-shell nanoparticles: A novel candidate for gene delivery
, Article Journal of Drug Delivery Science and Technology ; Volume 61 , 2021 ; 17732247 (ISSN) ; Mofazzal Jahromi, M. A ; Abdoli, A ; Mohammad Beigi, H ; Fatahi, Y ; Nourizadeh, H ; Zare, H ; Kiani, J ; Radmanesh, F ; Rabiee, N ; Jahani, M ; Mombeiny, R ; Karimi, M ; Sharif University of Technology
Editions de Sante
2021
Abstract
Cationic polymers such as poly-L-lysine (PLL) are able to interact electrostatically with DNA to produce polymeric systems with nanometric diameters due to the neutralization and accumulation of DNA. This study integrates the outstanding properties of carbon quantum dots (CQDs) with PLL to develop a novel gene delivery vehicle with a core-shell hybrid nanostructure. The CQD/PLL core-shell nanoparticles (NPs) were, therefore, synthesized in such a way that they had narrow size distribution and an average diameter under 10 nm, both of which were confirmed by dynamic light scattering (DLS) and transmission electron microscopy (TEM). Fourier transform infrared (FTIR) spectroscopy exhibited that...
Polysaccharide-based nanocomposites for biomedical applications: a critical review
, Article Nanoscale Horizons ; Volume 7, Issue 10 , 2022 , Pages 1136-1160 ; 20556756 (ISSN) ; Shokrani, A ; Sajadi, S. M ; Khodadadi Yazdi, M ; Seidi, F ; Jouyandeh, M ; Zarrintaj, P ; Kar, S ; Kim, S. J ; Kuang, T ; Rabiee, N ; Hejna, A ; Saeb, M. R ; Ramakrishna, S ; Sharif University of Technology
Royal Society of Chemistry
2022
Abstract
Polysaccharides (PSA) have taken specific position among biomaterials for advanced applications in medicine. Nevertheless, poor mechanical properties are known as the main drawback of PSA, which highlights the need for PSA modification. Nanocomposites PSA (NPSA) are a class of biomaterials widely used as biomedical platforms, but despite their importance and worldwide use, they have not been reviewed. Herein, we critically reviewed the application of NPSA by categorizing them into generic and advanced application realms. First, the application of NPSA as drug and gene delivery systems, along with their role in the field as an antibacterial platform and hemostasis agent is discussed. Then,...
Zn-rich (GaN)1−x(ZnO)x: a biomedical friend?
, Article New Journal of Chemistry ; Volume 45, Issue 8 , 2021 , Pages 4077-4089 ; 11440546 (ISSN) ; Rabiee, N ; Fatahi, Y ; Dinarvand, R ; Sharif University of Technology
Royal Society of Chemistry
2021
Abstract
A Zn-Rich (GaN)1−x(ZnO)xnanostructure was synthesized with the assistance of a high-gravity technique in order to reduce the reaction time and temperature. The synthesized inorganic nanomaterial has been applied in both drug and gene delivery systems, and as the first fully inorganic nanomaterial, it was investigated in a comprehensive cellular investigation as well. In order to increase the potential bioavailability, as well as the interactions with the pCRISPR, the nanomaterial was enriched with additional Zn ions. The nanomaterial and the final nanocarrier were characterized at each step before and after any biological analysisviaFESEM, AFM, TEM, FTIR and XRD. The polymer coated...
Smart nanostructures for cargo delivery: uncaging and activating by light
, Article Journal of the American Chemical Society ; Volume 139, Issue 13 , 2017 , Pages 4584-4610 ; 00027863 (ISSN) ; Sahandi Zangabad, P ; Baghaee Ravari, S ; Ghazadeh, M ; Mirshekari, H ; Hamblin, M. R ; Sharif University of Technology
Abstract
Nanotechnology has begun to play a remarkable role in various fields of science and technology. In biomedical applications, nanoparticles have opened new horizons, especially for biosensing, targeted delivery of therapeutics, and so forth. Among drug delivery systems (DDSs), smart nanocarriers that respond to specific stimuli in their environment represent a growing field. Nanoplatforms that can be activated by an external application of light can be used for a wide variety of photoactivated therapies, especially light-triggered DDSs, relying on photoisomerization, photo-cross-linking/un-cross-linking, photoreduction, and so forth. In addition, light activation has potential in photodynamic...