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Label-free and simple detection of endotoxins using a sensitive LSPR biosensor based on silver nanocolumns
, Article Analytical Biochemistry ; Volume 548 , 2018 , Pages 96-101 ; 00032697 (ISSN) ; Hosseini, N ; Vossoughi, M ; Khatami, M ; Abbasian, S ; Moshaii, A ; Sharif University of Technology
Academic Press Inc
2018
Abstract
This paper describes the construction of a silver-based LSPR biosensor for endotoxin detection. We used GLAD method to procure reproducible silver nanocolumns. In this work, the silver nanostructures were considerably stabilized by a SAM of MPA, and the limit of detection of biosensor was measured to be 340 pg/ml for endotoxin E. coli. Considering endotoxin B. abortus as the second type of endotoxin contamination in our target samples (HBs-ag produced in Institute Pasteur, Iran), we investigated selectivity of the biosensor in various experiments. We showed that this biosensor can selectively detect both types of endotoxins compared to other biological species. Overall, this study proposes...