Search for: glucose-concentration
Biodegradation of cyanide under alkaline conditions by a strain of pseudomonas putida isolated from gold mine soil and optimization of process variables through response surface methodology (RSM), Article Periodica Polytechnica Chemical Engineering ; Volume 62, Issue 3 , May , 2018 , Pages 265-273 ; 03245853 (ISSN) ; Yaghmaei, S ; Ghobadi Nejad, Z ; Sharif University of Technology
Budapest University of Technology and Economics 2018
In regard to highly poisonous effects of cyanide ion, concerns have been focused recently on treatment of such compounds in different ways. Four bacterial strains (C1-C4) capable of using cyanide as nitrogen source were isolated from contaminated gold mine soil samples under alkaline conditions at 30 °C, pH 9.5-10.5, and agitation speed 150 rpm. The gram-negative bacterium C3 (identified as Pseudomonas parafulva NBRC 16636(T) by 16S rRNA gene sequencing) was able to tolerate cyanide up to 500 ppm besides removing 93.5% of 200 ppm cyanide in 13 days which was confirmed by microorganisms growth. The addition of basal salts enhanced the removal efficiency of C3 by 16%. Cyanide removal...
Article Scientia Iranica ; Volume 16, Issue 2 C , 2009 , Pages 145-148 ; 10263098 (ISSN) ; Razavi, J ; Yaghmaei, S ; Sharif University of Technology
The effect of glucose concentration in submerged fermentation (SMF) on chitosan production by Aspergillus niger was investigated. A. niger, BBRC, 20004, from the Biochemical and Bioenvironmental Research Centre at Sharif University of Technology, Tehran, Iran, was grown in a Sabouro Dextrose media. Chitosan was extracted from the fungal mycelia using hot alkaline and acid treatment and after 12 days of cultivation, 0.8455 g chitosan /l of the fermentation medium was obtained. The content of glucose in the Sobouro Dextrose Broth media was also changed and the highest yield of chitosan 0.9121 g/l was obtained in Sobouro Dextrose Broth media containing 8% glucose. © Sharif University of...
Fabrication of a microdialysis-based nonenzymatic microfluidic sensor for regular glucose measurement, Article Sensors and Actuators, B: Chemical ; Volume 333 , 2021 ; 09254005 (ISSN) ; Saidi, M. S ; Shahrokhian, S ; Hosseini, H ; Kazemzadeh Hannani, S ; Sharif University of Technology
Elsevier B.V 2021
Microdialysis-based continuous glucose measuring systems are desirable candidates for accurate and biologically safe monitoring of glucose level in diabetic patients. However, it is necessary to improve these systems by utilizing highly reliable non-enzymatic sensors instead of enzymatic ones, while lowering the size and lessening the dialysis fluid consumption. Our purpose is to design an implantable integrated microfluidic device for regular nonenzymatic microdialysis-based glucose measurement. We report a novel nonenzymatic microfluidic glucose sensor based on Pt-Ni nanoparticles - multiwalled carbon nanotubes/screen-printed carbon electrode (Pt-Ni NPs-MWCNTs/SPE). Devised microfluidic...
Development of a non-invasive micron sized blood glucose sensor based on microsphere stimulated raman spectroscopy, Article Sensors and Transducers ; Volume 147, Issue 12 , 2012 , Pages 129-142 ; 17265479 (ISSN) ; Jahangiri, N ; Taraz, M ; Sharif University of Technology
We proposed a new method for Non-invasive measuring of blood glucose levels by using micro sphere Stimulated Raman scattering. We show that this method can be used to measure biological glucose levels with low power lasers. The field enhancement due to the resonance condition of high quality factor microsphere causes to reduce stimulated Raman scattering thresholds of its surrounding media (glucose) and hence observing stimulated Raman scattering with low power. The results from theoretical studies indicate that the stimulated Raman signal amplitude levels produced from the very low Glucose concentrations. By measuring the changes of the signal intensity in output of fiber, we can determine...
Enhancement of fungal delignification of rice straw by Trichoderma viride sp. to improve its saccharification, Article Biochemical Engineering Journal ; Volume 101 , September , 2015 , Pages 77-84 ; 1369703X (ISSN) ; Karimi, M ; Biria, D ; Kariminia, H. R ; Jeihanipour, A ; Sharif University of Technology
Fungal delignification can be considered as a feasible process to pre-treat lignocellulosic biomass in biofuel production, if its performance is improved in terms of efficiency thorough a few modifications. In this study, Trichoderma viride was utilized to investigate the effect of wet-milling, addition of surfactant (Tween 80) and optimization of operating factors such as temperature, biomass to liquid medium ratio and glucose concentration on biodelignification of rice straw. Next, the enzymatic hydrolysis of pretreated biomass was studied at various pretreatment times. Results revealed that the wet milling and addition of surfactant increases the lignin removal about 15% and 11%,...
Effect of formulation factors on the bioactivity of glucose oxidase encapsulated chitosan-alginate microspheres: In vitro investigation and mathematical model prediction, Article Chemical Engineering Science ; Volume 125 , March , 2015 , Pages 4-12 ; 00092509 (ISSN) ; Cheng, J ; Wu, X. Y ; Sharif University of Technology
Elsevier Ltd 2015
Higher reactive oxygen species (ROS) levels in cancer cells than normal cells have long been recognized, which makes cancer cells more susceptible to excess ROS. Thus oxidation (also called pro-oxidant) therapy has been explored as new cancer therapy regimens. To produce additional ROS, e.g. H2O2 in situ within tumor, we encapsulated glucose oxidase in chitosan-coated alginate-calcium microspheres (GOX-MS) for locoregional treatment and demonstrated its efficacy against cancer cells in vitro and in vivo. Owing to the complex biological functions of ROS, the production rate and amount of H2O2 are critical to achieve therapeutic benefits without causing normal tissue toxicity. This work was...
Response surface methodology as an approach to optimize growth medium of indigenous strain of Bacillus mycoides for production of biosurfactant, Article ICBEE 2010 - 2010 2nd International Conference on Chemical, Biological and Environmental Engineering, Proceedings, 2 November 2010 through 4 November 2010 ; 2010 , Pages 146-152 ; 9781424487479 (ISBN) ; Rahimpour, M. R ; Jahanmiri, A. H ; Roostaazad, R ; Arabian, D ; Soleimani, M ; Sharif University of Technology
In this study, we have investigated the potential of a native bacterial strain isolated from an Iranian oil field for the production of biosurfactant. The bacterium was identified to be Bacillus mycoides by biochemical tests and 16S ribotyping. The biosurfactant, which was produced by this bacterium, was able to reduce the surface tension of media to 34 mN/m. Biosurfactant production was optimized by the combination of central composite design (CCD) and response surface methodology (RSM). The factor selected for optimization of growth conditions were pH, temperature, glucose and salinity concentrations. The empirical model developed through RSM in terms of effective operational factors...
Prediction of necrotic core and hypoxic zone of multicellular spheroids in a microbioreactor with a U-shaped barrier, Article Micromachines ; Volume 9, Issue 3 , 2018 ; 2072666X (ISSN) ; Saidi, M. S ; Kashaninejad, N ; Nguyen, N. T ; Sharif University of Technology
MDPI AG 2018
Microfluidic devices have been widely used for biological and cellular studies. Microbioreactors for three-dimensional (3D) multicellular spheroid culture are now considered as the next generation in in vitro diagnostic tools. The feasibility of using 3D cell aggregates to form multicellular spheroids in a microbioreactor with U-shaped barriers has been demonstrated experimentally. A barrier array is an alternative to commonly used microwell traps. The present study investigates oxygen and glucose concentration distributions as key parameters in a U-shaped array microbioreactor using finite element simulation. The effect of spheroid diameter, inlet concentration and flow rate of the medium...
Glucose cross-linked hydrogels conjugate HA nanorods as bone scaffolds: Green synthesis, characterization and in vitro studies, Article Materials Chemistry and Physics ; Volume 242 , 2020 ; Shafiei, N ; Mohandes, F ; Dolatyar, B ; Zandi, N ; Zeynali, B ; Simchi, A ; Sharif University of Technology
Elsevier Ltd 2020
In the expanding field of tissue engineering (TE), improvement of biodegradability and osteoconductivity of biomaterials are required. The use of non-toxic reagents during manufacturing processes is also necessary to decrease toxicity and increase cell viability in vivo. Herein, we present a novel approach to prepare hydroxyapatite (HA) nanorods from sea bio-wastes through a green and eco-friendly wet-chemical processing for bone TE. Highly porous natural polymer-ceramic nanocomposites made of HA, gelatin (Ge) and carboxymethyl cellulose (CMC) hydrogels are then introduced. It was found that cross-linking of the hydrogel matrix by glucose as a green reagent affected all characteristics of...
Delay-Independent regulation of blood glucose for type-1 diabetes mellitus patients via an observer-based predictor feedback approach by considering quantization constraints, Article European Journal of Control ; Volume 63 , January , 2021 , Pages 240-252 ; 09473580 (ISSN) ; Tavazoei, M. S ; Sharif University of Technology
Elsevier Ltd 2021
Diabetes, as a widespread chronic disease, is caused by the increase of blood glucose concentration (BGC) due to pancreatic insulin production failure and/or insulin resistance in the body. The artificial pancreas (AP) known as a closed-loop insulin delivery control system consists of a glucose sensor for BGC measurement, a control algorithm for calculation of exogenous insulin delivery rate (IDR), and an insulin infusion pump. The AP provides a closed-loop glucose-insulin regulatory system for type-1 diabetes mellitus (T1DM) patients in order to effectively reduce the high BGC level. In this paper, we aim to design a controller in order to regulate the BGC of T1DM patients at its basal...
Enhancing biosurfactant production from an indigenous strain of Bacillus mycoides by optimizing the growth conditions using a response surface methodology, Article Chemical Engineering Journal ; Volume 163, Issue 3 , October , 2010 , Pages 188-194 ; 13858947 (ISSN) ; Rahimpour, M. R ; Jahanmiri, A. H ; Roostaazad, R ; Arabian, D ; Ghobadi, Z ; Sharif University of Technology
In this study, we have investigated the potential of a native bacterial strain isolated from an Iranian oil field for the production of biosurfactant. The bacterium was identified to be Bacillus mycoides by biochemical tests and 16S ribotyping. The biosurfactant, which was produced by this bacterium, was able to reduce the surface tension of media to 34. mN/m. Compositional analysis of the produced biosurfactant has been carried out by thin layer chromatography (TLC) and FT-IR. The biosurfactant produced by the isolate was characterized as lipopeptide derivative. Biosurfactant production was optimized by the combination of central composite design (CCD) and response surface methodology...
Article Journal of Membrane Science ; Volume 335, Issue 1-2 , 2009 , Pages 21-31 ; 03767388 (ISSN) ; Wu, X. Y ; Sharif University of Technology
A theoretical model was developed to describe a dynamic process involving an enzymatic reaction and diffusion of reactants and product inside glucose sensitive composite membrane. The composite membrane consisted of nanoparticles of a weakly acidic polymer, glucose oxidase and catalase embedded in a hydrophobic polymer. Time- and position-dependent diffusivity of involved species was considered in the model. Donnan equilibrium was used to find concentrations of buffer ions inside the membrane. The profiles of pH, species concentrations, volume fraction of swollen gel, polymer and water-filled space, as well as solute diffusivity inside the membrane were predicted by the model as a function...
Interactive optimization of biosurfactant production by Paenibacillus alvei ARN63 isolated from an Iranian oil well, Article Colloids and Surfaces B: Biointerfaces ; Volume 82, Issue 1 , 2011 , Pages 33-39 ; 09277765 (ISSN) ; Rahimpour, M. R ; Jahanmiri, A. H ; Roostaazad, R ; Arabian, D ; Soleimani, M ; Jamshidnejad, Z ; Sharif University of Technology
The potential of an indigenous bacterial strain isolated from an Iranian oil field for the production of biosurfactant was investigated in this study. After isolation, the bacterium was characterized to be Paenibacillus alvei by biochemical tests and 16S ribotyping. The biosurfactant, which was produced by this bacterium, was able to lower the surface tension of media to 35. mN/m. Accordingly, thin layer chromatography (TLC) and FT-IR has been carried out to determine compositional analysis of the produced biosurfactant. After all the tests related to characterization of the biosurfactant produced by the isolated bacterium, it was characterized as lipopeptide derivative. The combination of...