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    Cyto and genotoxicities of graphene oxide and reduced graphene oxide sheets on spermatozoa

    , Article RSC Advances ; Vol. 4, issue. 52 , May , 2014 , pp. 27213-27223 ; ISSN: 20462069 Hashemi, E ; Akhavan, O ; Shamsara, M ; Rahighi, R ; Esfandiar, A ; Tayefeh, A. R ; Sharif University of Technology
    Concentration-dependent cyto and genotoxicities of graphene oxide (GO) and reduced GO (rGO) sheets on spermatozoa were studied. rGO sheets with various surface chemical states were achieved using hydrazine (N2H 4) hydrothermal (HT) reactions and green tea polyphenols (GTPs). Although 0.1 μg mL-1 graphene could not change sperm viability and kinetic parameters, <40% and 20% of spermatozoa were viable and progressively motile, after 2 h incubation with 400 μg mL-1 GO or rGO, respectively. All the graphene nanomaterials induced concentration- dependent reductions of adenosine triphosphate and NAD+/NADH produced by spermatozoa for motility and metabolic activity. While GO, N 2H4-rGO, and HT-rGO... 

    Increasing the antioxidant activity of green tea polyphenols in the presence of iron for the reduction of graphene oxide

    , Article Carbon ; Volume 50, Issue 8 , 2012 , Pages 3015-3025 ; 00086223 (ISSN) Akhavan, O ; Kalaee, M ; Alavi, Z. S ; Ghiasi, S. M. A ; Esfandiar, A ; Sharif University of Technology
    An easy method for green and low-temperature (40 °C) reduction of graphene oxide (GO) by increasing the antioxidant activity of green tea polyphenols (GTPs) in the presence of iron was developed. The reduction level (obtained by X-ray photoelectron spectroscopy) and electrical conductivity (obtained by current-voltage measurement) of the GO sheets reduced by GTPs in the presence of iron were comparable to those of hydrazine-reduced GO and much better than those of the GO reduced by only GTPs (in the absence of iron) at reduction temperatures of 40-80 °C. Raman spectroscopy indicated that application of GTPs in the presence of iron, in contrast to hydrazine, resulted in better recovering of... 

    Polyphenols attached graphene nanosheets for high efficiency NIR mediated photodestruction of cancer cells

    , Article Materials Science and Engineering C ; Volume 33, Issue 3 , 2013 , Pages 1498-1505 ; 09284931 (ISSN) Abdolahad, M ; Janmaleki, M ; Mohajerzadeh, S ; Akhavan, O ; Abbasi, S ; Sharif University of Technology
    Green tea-reduced graphene oxide (GT-rGO) sheets have been exploited for high efficiency near infrared (NIR) photothermal therapy of HT29 and SW48 colon cancer cells. The biocompatibility of GT-rGO sheets was investigated by means of MTT assays. The polyphenol constituents of GT-rGO act as effective targeting ligands for the attachment of rGO to the surface of cancer cells, as confirmed by the cell granularity test in flow cytometry assays and also by scanning electron microscopy. The photo-thermal destruction of higher metastatic cancer cells (SW48) is found to be more than 20% higher than that of the lower metastatic one (HT29). The photo-destruction efficiency factor of the GT-rGO is...