Loading...
Search for:
heart-attack-risk
0.005 seconds
Multilayered mesoporous composite nanostructures for highly sensitive label-free quantification of cardiac troponin-i
, Article Biosensors ; Volume 12, Issue 5 , 2022 ; 20796374 (ISSN) ; Amidian, M. A ; Sheybanikashani, S ; Mahdavi, H ; Alimohammadi, H ; Syedmoradi, L ; Mohandes, F ; Zarrabi, A ; Tamjid, E ; Omidfar, K ; Simchi, A ; Sharif University of Technology
MDPI
2022
Abstract
Cardiac troponin-I (cTnI) is a well-known biomarker for the diagnosis and control of acute myocardial infarction in clinical practice. To improve the accuracy and reliability of cTnI electrochemical immunosensors, we propose a multilayer nanostructure consisting of Fe3O4-COOH labeled anti-cTnI monoclonal antibody (Fe3O4-COOH-Ab1 ) and anti-cTnI polyclonal antibody (Ab2 ) conjugated on Au-Ag nanoparticles (NPs) decorated on a metal–organic framework (Au-Ag@ZIF-67-Ab2 ). In this design, Fe3O4-COOH was used for separation of cTnI in specimens and signal amplification, hierarchical porous ZIF-67 extremely enhanced the specific surface area, and Au-Ag NPs synergically promoted the conductivity...