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Anti-HER2 VHH targeted magnetoliposome for intelligent magnetic resonance imaging of breast cancer cells
, Article Cellular and Molecular Bioengineering ; Volume 10, Issue 3 , 2017 , Pages 263-272 ; 18655025 (ISSN) ; Rahbarizadeh, F ; Ahmadvand, D ; Madaah Hosseini, H. R ; Sharif University of Technology
Abstract
The combination of liposomes with magnetic nanoparticles, because of their strong effect on T2 relaxation can open new ways in the innovative cancer therapy and diagnosis. In order to design an intelligent contrast agent in MRI, we chose anti-HER2 nanobody the smallest fully functional antigen-binding fragments evolved from the variable domain, the VHH, of a camel heavy chain-only antibody. These targeted magnetoliposomes bind to the HER2 antigen which is highly expressed on breast and ovarian cancer cells so reducing the side effects as well as increasing image contrast and effectiveness. Cellular iron uptake analysis and in vitro MRI of HER2 positive cells incubated with targeted...