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Temperature: the "ignored" factor at the nanobio interface
, Article ACS Nano ; Volume 7, Issue 8 , 2013 , Pages 6555-6562 ; 19360851 (ISSN) ; Abdelmonem, A. M ; Behzadi, S ; Clement, J. H ; Dutz, S ; Ejtehadi, M. R ; Hartmann, R ; Kantner, K ; Linne, U ; Maffre, P ; Metzler, S ; Moghadam, M. K ; Pfeiffer, C ; Rezaei, M ; Ruiz-Lozano, P ; Serpooshan, V ; Shokrgozar, M. A ; Nienhaus, G. U ; Parak, W. J ; Sharif University of Technology
2013
Abstract
Upon incorporation of nanoparticles (NPs) into the body, they are exposed to biological fluids, and their interaction with the dissolved biomolecules leads to the formation of the so-called protein corona on the surface of the NPs. The composition of the corona plays a crucial role in the biological fate of the NPs. While the effects of various physicochemical parameters on the composition of the corona have been explored in depth, the role of temperature upon its formation has received much less attention. In this work, we have probed the effect of temperature on the protein composition on the surface of a set of NPs with various surface chemistries and electric charges. Our results...
Folic acid-adorned curcumin-loaded iron oxide nanoparticles for cervical cancer
, Article ACS Applied Bio Materials ; Volume 5, Issue 3 , 2022 , Pages 1305-1318 ; 25766422 (ISSN) ; Azarian, M ; Heydari Sheikh Hossein, H ; Abdolvahabi, Z ; Mohammadi Abgarmi, Z ; Moradi, A ; Mousavi, S. M ; Ashrafizadeh, M ; Makvandi, P ; Saeb, M. R ; Rabiee, N ; Sharif University of Technology
American Chemical Society
2022
Abstract
Cancer is a deadly disease that has long plagued humans and has become more prevalent in recent years. The common treatment modalities for this disease have always faced many problems and complications, and this has led to the discovery of strategies for cancer diagnosis and treatment. The use of magnetic nanoparticles in the past two decades has had a significant impact on this. One of the objectives of the present study is to introduce the special properties of these nanoparticles and how they are structured to load and transport drugs to tumors. In this study, iron oxide (Fe3O4) nanoparticles with 6 nm sizes were coated with hyperbranched polyglycerol (HPG) and folic acid (FA). The...
Cell "vision": Complementary factor of protein corona in nanotoxicology
, Article Nanoscale ; Volume 4, Issue 17 , 2012 , Pages 5461-5468 ; 20403364 (ISSN) ; Saeedi-Eslami, S. N ; Shokrgozar, M. A ; Azadmanesh, K ; Hassanlou, M ; Kalhor, H. R ; Burtea, C ; Rothen Rutishauser, B ; Laurent, S ; Sheibani, S ; Vali, H ; Sharif University of Technology
RSC
2012
Abstract
Engineered nanoparticles are increasingly being considered for use as biosensors, imaging agents and drug delivery vehicles. Their versatility in design and applications make them an attractive proposition for new biological and biomedical approaches. Despite the remarkable speed of development in nanoscience, relatively little is known about the interaction of nanoscale objects with living systems. In a biological fluid, proteins associate with nanoparticles, and the amount and the presentation of the proteins on their surface could lead to a different in vivo response than an uncoated particle. Here, in addition to protein adsorption, we are going to introduce concept of cell "vision",...
Synthesis and characterization of magnetic hybrid nanomaterials via RAFT polymerization: A pH sensitive drug delivery system
, Article Colloids and Surfaces B: Biointerfaces ; Volume 174 , 2019 , Pages 153-160 ; 09277765 (ISSN) ; Kohestanian, M ; Shirzad, M ; Sharif University of Technology
Elsevier B.V
2019
Abstract
Herein, a facile and versatile method for the synthesis of a novel magnetic nanocarrier via surface- initiated reversible addition-fragmentation chain transfer (RAFT) polymerization is introduced. At first, RAFT agent was successfully attached to the surface of Fe 3 O 4 nanoparticles and, then, poly (glycidyl methacrylate) (PGMA) chains were grown and anchored onto the surface of Fe 3 O 4 nanoparticles. At the end, hydrazine (Hy) groups were introduced to the PGMA chains via reaction between epoxy rings and hydrazine molecules. Doxorubicin (DOX) was covalently conjugated to the prepared nanocarrier (Fe 3 O 4 @PGMA@Hy) through a hydrazone linkage. The in vitro drug release of Fe 3 O 4...
Poly(glycidyl methacrylate)-coated magnetic graphene oxide as a highly efficient nanocarrier: Preparation, characterization, and targeted DOX delivery
, Article New Journal of Chemistry ; Volume 43, Issue 47 , 2019 , Pages 18647-18656 ; 11440546 (ISSN) ; Kohestanian, M ; Yaghoubi, M ; Sharif University of Technology
Royal Society of Chemistry
2019
Abstract
Herein, we report the preparation of novel magnetic graphene oxide (GO) grafted with brush polymer via surface-initiated reversible addition-fragmentation chain transfer (SI-RAFT) polymerization and its application as a nanocarrier for magnetic- and pH-triggered delivery of doxorubicin anticancer drug. The RAFT agent, DDMAT, was firstly attached to the surface of magnetic GO nanosheets. The DDMAT-functionalized magnetic GO nanosheets were then used to polymerize glycidyl methacrylate (GMA) using an SI-RAFT method. Afterwards, the epoxy rings of the PGMA chains were opened with hydrazine (N2H4) moieties. The resulting nanocomposite was used as a drug carrier for doxorubicin (DOX) as an...
Poly(glycidyl methacrylate)-coated magnetic graphene oxide as a highly efficient nanocarrier: Preparation, characterization, and targeted DOX delivery
, Article New Journal of Chemistry ; Volume 43, Issue 47 , 2019 , Pages 18647-18656 ; 11440546 (ISSN) ; Kohestanian, M ; Yaghoubi, M ; Sharif University of Technology
Royal Society of Chemistry
2019
Abstract
Herein, we report the preparation of novel magnetic graphene oxide (GO) grafted with brush polymer via surface-initiated reversible addition-fragmentation chain transfer (SI-RAFT) polymerization and its application as a nanocarrier for magnetic- and pH-triggered delivery of doxorubicin anticancer drug. The RAFT agent, DDMAT, was firstly attached to the surface of magnetic GO nanosheets. The DDMAT-functionalized magnetic GO nanosheets were then used to polymerize glycidyl methacrylate (GMA) using an SI-RAFT method. Afterwards, the epoxy rings of the PGMA chains were opened with hydrazine (N2H4) moieties. The resulting nanocomposite was used as a drug carrier for doxorubicin (DOX) as an...
Natural polymers decorated mof-mxene nanocarriers for co-delivery of doxorubicin/pCRISPR
, Article ACS Applied Bio Materials ; Volume 4, Issue 6 , 2021 , Pages 5106-5121 ; 25766422 (ISSN) ; Bagherzadeh, M ; Jouyandeh, M ; Zarrintaj, P ; Saeb, M. R ; Mozafari, M ; Shokouhimehr, M ; Varma, R. S ; Sharif University of Technology
American Chemical Society
2021
Abstract
A one-pot and facile method with assistance of high gravity was applied for the synthesis of inorganic two-dimensional MOF-5 embedded MXene nanostructures. The innovative inorganic MXene/MOF-5 nanostructure was applied in co-delivery of drug and gene, and to increase its bioavailability and interaction with the pCRISPR, the nanomaterial was coated with alginate and chitosan. The polymer-coated nanosystems were fully characterized, and the sustained DOX delivery and comprehensive cytotoxicity studies were conducted on the HEK-293, PC12, HepG2, and HeLa cell lines, demonstrating acceptable and excellent cell viability at both very low (0.1 μg.mL-1) and high (10 μg·mL-1) concentrations. The...
Structural characterization of a rhamnolipid-type biosurfactant produced by Pseudomonas aeruginosa MR01: Enhancement of di-rhamnolipid proportion using gamma irradiation
, Article Colloids and Surfaces B: Biointerfaces ; Volume 81, Issue 2 , 2010 , Pages 397-405 ; 09277765 (ISSN) ; Abassi, H ; Ahmadkhaniha, R ; Roostaazad, R ; Masoomi, F ; Zahiri, H. S ; Ahmadian, G ; Vali, H ; Noghabi, K. A ; Sharif University of Technology
2010
Abstract
We previously reported that MR01, an indigenous strain of Pseudomonas aeruginosa, was able to produce a rhamnolipid-type biosurfactant. Here, we attempted to define the structural properties of this natural product. The analysis of the extracted biosurfactant by thin-layer chromatography (TLC) revealed the presence of two compounds corresponding to those of authentic mono- and di-rhamnolipid. The identity of two structurally distinguished rhamnolipids was confirmed by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy. Liquid chromatography/mass spectrometry (LC/MS) of extracted biosurfactant revealed up to seventeen different rhamnolipid congeners. Further quantification showed...
Polymer-Coated NH2-UiO-66 for the codelivery of DOX/pCRISPR
, Article ACS Applied Materials and Interfaces ; Volume 13, Issue 9 , 2021 , Pages 10796-10811 ; 19448244 (ISSN) ; Bagherzadeh, M ; Heidarian Haris, M ; Ghadiri, A. M ; Matloubi Moghaddam, F ; Fatahi, Y ; Dinarvand, R ; Jarahiyan, A ; Ahmadi, S ; Shokouhimehr, M ; Sharif University of Technology
American Chemical Society
2021
Abstract
Herein, the NH2-UiO-66 metal organic framework (MOF) has been green synthesized with the assistance of high gravity to provide a suitable and safe platform for drug loading. The NH2-UiO-66 MOF was characterized using a field-emission scanning electron microscope, transmission electron microscope (TEM), X-ray diffraction, and zeta potential analysis. Doxorubicin was then encapsulated physically on the porosity of the green MOF. Two different stimulus polymers, p(HEMA) and p(NIPAM), were used as the coating agents of the MOFs. Doxorubicin was loaded onto the polymer-coated MOFs as well, and a drug payload of more than 51% was obtained, which is a record by itself. In the next step, pCRISPR was...
Synthesis of green benzamide-decorated UiO-66-NH2 for biomedical applications
, Article Chemosphere ; Volume 299 , 2022 ; 00456535 (ISSN) ; Ghadiri, A. M ; Alinezhad, V ; Sedaghat, A ; Ahmadi, S ; Fatahi, Y ; Makvandi, P ; Saeb, M. R ; Bagherzadeh, M ; Asadnia, M ; Varma, R. S ; Lima, E. C ; Sharif University of Technology
Elsevier Ltd
2022
Abstract
Metal-organic frameworks (MOFs) biocompatible systems can host enzymes/bacteria/viruses. Herein we synthesized a series of fatty acid amide hydrolase (FAAH)-decorated UiO-66-NH2 based on Citrus tangerine leaf extract for drug delivery and biosensor applications. Five chemically manipulated FAAH-like benzamides were localized on the UiO-66-NH2 surface with physical interactions. Comprehensive cellular and molecular analyses were conducted on HEK-293, HeLa, HepG2, PC12, MCF-7, and HT-29 cell lines (cytotoxicity assessment after 24 and 48 h). MTT results proved above 95 and 50% relative cell viability in the absence and presence of the drug, respectively. A complete targeted drug-releasing...
Synthesizing and staining manganese oxide nanoparticles for cytotoxicity and cellular uptake investigation
, Article Biochimica et Biophysica Acta - General Subjects ; Vol. 1840, Issue. 1 , 2014 , pp. 428-433 ; ISSN: 03044165 ; Oghabian, M. A ; Ahmadi, R ; Shahbazi, N ; Hosseini, H. R. M ; Shanehsazzadeh, S ; Zangeneh, R. N ; Sharif University of Technology
Abstract
Background For decades, contrast agents have been used to reduce longitudinal (T1) or transverse (T2) relaxation times. High toxicity of gadolinium-based contrast agents leads researchers to new T1 contrast agents. Manganese oxide (MnO) nanoparticle (NP) with the lower peril and good enough signal change ability has been offered as a new possibility for magnetic resonance imaging (MRI). Methods The synthesized NPs were investigated for physicochemical and biological properties by X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscope, dynamic light scattering (DLS), inductively coupled plasma, enzyme-linked immunosorbent assay, and 3 T magnetic resonance...
Turning toxic nanomaterials into a safe and bioactive nanocarrier for co-delivery of DOX/pCRISPR
, Article ACS Applied Bio Materials ; Volume 4, Issue 6 , 2021 , Pages 5336-5351 ; 25766422 (ISSN) ; Bagherzadeh, M ; Ghadiri, A. M ; Fatahi, Y ; Aldhaher, A ; Makvandi, P ; Dinarvand, R ; Jouyandeh, M ; Saeb, M. R ; Mozafari, M ; Shokouhimehr, M ; Hamblin, M. R ; Varma, R. S ; Sharif University of Technology
American Chemical Society
2021
Abstract
Hybrid bioactive inorganic-organic carbon-based nanocomposites of reduced graphene oxide (rGO) nanosheets enlarged with multi-walled carbon nanotubes (MWCNTs) were decorated to provide a suitable space forin situgrowth of CoNi2S4and green-synthesized ZnO nanoparticles. The ensuing nanocarrier supplied π-π interactions between the DOX drug and a stabilizing agent derived from leaf extracts on the surface of ZnO nanoparticles and hydrogen bonds; gene delivery of (p)CRISPR was also facilitated by chitosan and alginate renewable macromolecules. Also, these polymers can inhibit the potential interactions between the inorganic parts and cellular membranes to reduce the potential cytotoxicity....
Zn-rich (GaN)1−x(ZnO)x: a biomedical friend?
, Article New Journal of Chemistry ; Volume 45, Issue 8 , 2021 , Pages 4077-4089 ; 11440546 (ISSN) ; Rabiee, N ; Fatahi, Y ; Dinarvand, R ; Sharif University of Technology
Royal Society of Chemistry
2021
Abstract
A Zn-Rich (GaN)1−x(ZnO)xnanostructure was synthesized with the assistance of a high-gravity technique in order to reduce the reaction time and temperature. The synthesized inorganic nanomaterial has been applied in both drug and gene delivery systems, and as the first fully inorganic nanomaterial, it was investigated in a comprehensive cellular investigation as well. In order to increase the potential bioavailability, as well as the interactions with the pCRISPR, the nanomaterial was enriched with additional Zn ions. The nanomaterial and the final nanocarrier were characterized at each step before and after any biological analysisviaFESEM, AFM, TEM, FTIR and XRD. The polymer coated...
CaZnO-based nanoghosts for the detection of ssDNA, pCRISPR and recombinant SARS-CoV-2 spike antigen and targeted delivery of doxorubicin
, Article Chemosphere ; Volume 306 , 2022 ; 00456535 (ISSN) ; Akhavan, O ; Fatahi, Y ; Ghadiri, A. M ; Kiani, M ; Makvandi, P ; Rabiee, M ; Nicknam, M. H ; Saeb, M. R ; Varma, R. S ; Ashrafizadeh, M ; Nazarzadeh Zare, E ; Sharifi, E ; Lima, E. C ; Sharif University of Technology
Elsevier Ltd
2022
Abstract
Overexpression of proteins/antigens and other gene-related sequences in the bodies could lead to significant mutations and refractory diseases. Detection and identification of assorted trace concentrations of such proteins/antigens and/or gene-related sequences remain challenging, affecting different pathogens and making viruses stronger. Correspondingly, coronavirus (SARS-CoV-2) mutations/alterations and spread could lead to overexpression of ssDNA and the related antigens in the population and brisk activity in gene-editing technologies in the treatment/detection may lead to the presence of pCRISPR in the blood. Therefore, the detection and evaluation of their trace concentrations are of...
Bioactive hybrid metal-organic framework (MOF)-based nanosensors for optical detection of recombinant SARS-CoV-2 spike antigen
, Article Science of the Total Environment ; Volume 825 , 2022 ; 00489697 (ISSN) ; Fatahi, Y ; Ahmadi, S ; Abbariki, N ; Ojaghi, A ; Rabiee, M ; Radmanesh, F ; Dinarvand, R ; Bagherzadeh, M ; Mostafavi, E ; Ashrafizadeh, M ; Makvandi, P ; Lima, E. C ; Saeb, M. R ; Sharif University of Technology
Elsevier B.V
2022
Abstract
Fast, efficient, and accurate detection of SARS-CoV-2 spike antigen is pivotal to control the spread and reduce the mortality of COVID-19. Nevertheless, the sensitivity of available nanobiosensors to detect recombinant SARS-CoV-2 spike antigen seems insufficient. As a proof-of-concept, MOF-5/CoNi2S4 is developed as a low-cost, safe, and bioactive hybrid nanostructure via the one-pot high-gravity protocol. Then, the porphyrin, H2TMP, was attached to the surface of the synthesized nanomaterial to increase the porosity for efficient detection of recombinant SARS-CoV-2 spike antigen. AFM results approved roughness in different ranges, including 0.54 to 0.74 μm and 0.78 to ≈0.80 μm, showing good...