Search for: hemolysis
High-throughput, label-free isolation of white blood cells from whole blood using parallel spiral microchannels with u-shaped cross-section, Article Biosensors ; Volume 11, Issue 11 , 2021 ; 20796374 (ISSN) ; Rostami, P ; Saidi, M. S ; Firoozabadi, B ; Kashaninejad, N ; Sharif University of Technology
Rapid isolation of white blood cells (WBCs) from whole blood is an essential part of any WBC examination platform. However, most conventional cell separation techniques are labor-intensive and low throughput, require large volumes of samples, need extensive cell manipulation, and have low purity. To address these challenges, we report the design and fabrication of a passive, label-free microfluidic device with a unique U-shaped cross-section to separate WBCs from whole blood using hydrodynamic forces that exist in a microchannel with curvilinear geometry. It is shown that the spiral microchannel with a U-shaped cross-section concentrates larger blood cells (e.g., WBCs) in the inner...
Pyromellitic dianhydride crosslinked cyclodextrin nanosponges for curcumin controlled release; formulation, physicochemical characterization and cytotoxicity investigations, Article Journal of Microencapsulation ; Volume 36, Issue 8 , 2019 , Pages 715-727 ; 02652048 (ISSN) ; Zarrabi, A ; Caldera, F ; Trotta, F ; Ghias, N ; Sharif University of Technology
Taylor and Francis Ltd 2019
Aim: In this study, a nanosponge structure was synthesised with capability of encapsulating curcumin as a model polyphenolic compound and one of the herbal remedies that have widely been considered due to its ability to treat cancer. Methods: FTIR, DSC and XRD techniques were performed to confirm the formation of the inclusion complex of the nanosponge-drug. Results: DSC and XRD patterns showed an increasing stability and a decreasing crystallinity of curcumin after formation of inclusion complex. Encapsulation efficiency was 98% (w/w) and a significant increase was observed in loading capacity (184% w/w). The results of cytotoxicity assessments demonstrated no cell toxicity on the healthy...
A new detection chamber design on centrifugal microfluidic platform to measure hemoglobin of whole blood, Article SLAS Technology ; Volume 26, Issue 4 , 2021 , Pages 392-398 ; 24726303 (ISSN) ; Saadatmand, M ; Eghbal, M ; Bakhtiari, M. R ; Mehraji, S ; Sharif University of Technology
SAGE Publications Inc 2021
Undoubtedly, microfluidics has been a focal point of interdisciplinary science during the last two decades, resulting in many developments in this area. Centrifugal microfluidic platforms have good potential for use in point-of-care devices because they take advantage of some intrinsic forces, most notably centrifugal force, which obviates the need to any external driving forces. Herein, we introduce a newly designed detection chamber for use on microfluidic discs that can be employed as an absorbance readout step in cases where the final solution has a very low viscosity and surface tension. In such situations, our chamber easily eliminates the air bubbles from the final solution without...
Delivery of melittin-loaded niosomes for breast cancer treatment: an in vitro and in vivo evaluation of anti-cancer effect, Article Cancer Nanotechnology ; Volume 12, Issue 1 , 2021 ; 18686958 (ISSN) ; Akbarzadeh, I ; Marzbankia, E ; Farid, M ; khaledi, L ; Reihani, A. H ; Javidfar, M ; Mortazavi, P ; Sharif University of Technology
BioMed Central Ltd 2021
Background: Melittin, a peptide component of honey bee venom, is an appealing candidate for cancer therapy. In the current study, melittin, melittin-loaded niosome, and empty niosome had been optimized and the anticancer effect assessed in vitro on 4T1 and SKBR3 breast cell lines and in vivo on BALB/C inbred mice. "Thin-layer hydration method" was used for preparing the niosomes; different niosomal formulations of melittin were prepared and characterized in terms of morphology, size, polydispersity index, encapsulation efficiency, release kinetics, and stability. A niosome was formulated and loaded with melittin as a promising drug carrier system for chemotherapy of the breast cancer cells....