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hepatocellular-carcinoma
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Numerical investigations of hepatic spheroids metabolic reactions in a perfusion bioreactor
, Article Frontiers in Bioengineering and Biotechnology ; Volume 7 , 2019 ; 22964185 (ISSN) ; Firoozabadi, B ; Firoozbakhsh, K ; Sharif University of Technology
Frontiers Media S.A
2019
Abstract
Miniaturized culture systems of hepatic cells are emerging as a strong tool facilitating studies related to liver diseases and drug discovery. However, the experimental optimization of various parameters involved in the operation of these systems is time-consuming and expensive. Hence, developing numerical tools predicting the function of such systems can significantly reduce the associated cost. In this paper, a perfusion-based three dimensional (3D) bioreactor comprising encapsulated human liver hepatocellular carcinoma (HepG2) spheroids are analyzed. The flow and mass transfer equations for oxygen as well as different metabolites such as albumin, glucose, glutamine, ammonia, and urea were...
A hepatocellular carcinoma–bone metastasis-on-a-chip model for studying thymoquinone-loaded anticancer nanoparticles
, Article Bio-Design and Manufacturing ; Volume 3, Issue 3 , 2020 , Pages 189-202 ; Yesil Celiktas, O ; Kazan, A ; Maharjan, S ; Saghazadeh, S ; Firoozbakhsh, K ; Firoozabadi, B ; Zhang, Y. S ; Sharif University of Technology
Springer
2020
Abstract
We report the development of a metastasis-on-a-chip platform to model and track hepatocellular carcinoma (HCC)–bone metastasis and to analyze the inhibitory effect of an herb-based compound, thymoquinone (TQ), in hindering the migration of liver cancer cells into the bone compartment. The bioreactor consisted of two chambers, one accommodating encapsulated HepG2 cells and one bone-mimetic niche containing hydroxyapatite (HAp). Above these chambers, a microporous membrane was placed to resemble the vascular barrier, where medium was circulated over the membrane. It was observed that the liver cancer cells proliferated inside the tumor microtissue and disseminated from the HCC chamber to the...