Sharif Digital Repository

A simple ultrasound-assisted dispersive micro-solid phase extraction method based on graphene-oxide (GO) nanosorbents (UAD-μ-SPE), followed by high performance liquid chromatography-ultraviolet detection (HPLC-UV) was developed for the simultaneous determination of theophylline, theobromine and caffeine in different infusion tea samples. GO was synthesized by Hummers method and characterized with field emission-scanning electron microscopy (FE-SEM), X-ray diffraction (XRD), and Fourier transform infrared (FT-IR) spectroscopy. The GO sorbent was dispersed in the sample solution with the assistance of ultrasound to enhance adsorption and desorption performance and rates. The main parameters... 

Experimental design of directly suspended droplet liquid-liquid-liquid microextraction has been used to determine residue of 2,4-D, in environmental water samples. A free suspended droplet of 5.5 μL of receiving phase is delivered to the top-center position of 1-Octanol, which is an immiscible organic solvent, floating on the top of a 2.5 mL aqueous sample while being agitated by a stirring bar to create a mild vortex at the center of the vial. Central composite rotatable design has been used for studying the effect of the parameters, the factors interacting with each other and finding the optimum condition. The chromatographic separation was accomplished on a shim-Pak C18 column using a... 

A crude phenolic glycolipid extract from Mycobacterium bovis BCG was fractionated by column chromatography. A reversed-phase high performance liquid chromatography (HPLC) method with UV detection at 275nm was developed for simultaneous detection and separation of phenolic glycolipids (PGLs) in Mycobacterium bovis BCG. This analysis provides a good resolution. Different solvent systems and columns for HPLC were compared. A system composed of acetonitrile-water in the ratio of 0→80% at a flow rate of 0.8 mL/min and C8 analytical column were found to be optimum for HPLC of the phenolic glycolipids. This simple method is therefore appropriate to purify these compounds present in M. bovis extract... 

In this study, a method was described for the extraction of three antibiotic residues from cow milk samples using a graphene oxide–starch–based nanocomposite. The prepared nanocomposites were employed as an extractive phase for micro-solid phase extraction of antibiotics from cow milk samples. The extracted antibiotics, i.e. amoxicillin, ampicillin and cloxacillin, were subsequently analyzed by high-performance liquid chromatography–ultraviolet detection (HPLC–UV). Important variables associated with the extraction and desorption efficiency were optimized. High extraction efficiencies for the selected antibiotics were conveniently achieved using the starch–based nanocomposite as the... 

An electro membrane extraction (EME) methodology was utilized to study the isolation of some environmentally important pollutants, such as chlorophenols, from aquatic media based upon the electrokinetic migration process. The analytes were transported by application of an electrical potential difference over a supported liquid membrane (SLM). A driving force of 10 V was applied to extract the analytes through 1-octanol, used as the SLM, into a strongly alkaline solution. The alkaline acceptor solution was subsequently analyzed by high performance liquid chromatography-ultraviolet (HPLC-UV) detection. The parameters influencing electromigration, including volumes and pH of the donor and...