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    Fabrication and characterization of a starch-based nanocomposite scaffold with highly porous and gradient structure for bone tissue engineering

    , Article Biomedical Physics and Engineering Express ; Volume 4, Issue 5 , 2018 ; 20571976 (ISSN) Mirab, F ; Eslamian, M ; Bagheri, R ; Sharif University of Technology
    Institute of Physics Publishing  2018
    Starch based scaffolds are considered as promising biomaterials for bone tissue engineering. In this study, a highly porous starch/polyvinyl alcohol (PVA) based nanocomposite scaffold with a gradient pore structure was made by incorporating different bio-additives, including citric acid, cellulose nanofibers, and hydroxyapatite (HA) nanoparticles. The scaffold was prepared by employing unidirectional and cryogenic freeze-casting and subsequently freeze-drying methods. Fourier transform infrared (FTIR) spectroscopy confirmed the cross-linking of starch and PVA molecules through multiple esterification phenomenon in the presence of citric acid as a cross-linking agent. Field emission scanning... 

    Magnetoelectric nanocomposite scaffold for high yield differentiation of mesenchymal stem cells to neural-like cells

    , Article Journal of Cellular Physiology ; Volume 234, Issue 8 , 2019 , Pages 13617-13628 ; 00219541 (ISSN) Esmaeili, E ; Soleimani, M ; Ghiass, M. A ; Hatamie, S ; Vakilian, S ; Zomorrod, M. S ; Sadeghzadeh, N ; Vossoughi, M ; Hosseinzadeh, S ; Sharif University of Technology
    Wiley-Liss Inc  2019
    While the differentiation factors have been widely used to differentiate mesenchymal stem cells (MSCs) into various cell types, they can cause harm at the same time. Therefore, it is beneficial to propose methods to differentiate MSCs without factors. Herein, magnetoelectric (ME) nanofibers were synthesized as the scaffold for the growth of MSCs and their differentiation into neural cells without factors. This nanocomposite takes the advantage of the synergies of the magnetostrictive filler, CoFe 2 O 4 nanoparticles (CFO), and piezoelectric polymer, polyvinylidene difluoride (PVDF). Graphene oxide nanosheets were decorated with CFO nanoparticles for a proper dispersion in the polymer through... 

    Design and fabrication of injectable microcarriers composed of acellular cartilage matrix and chitosan

    , Article Journal of Biomaterials Science, Polymer Edition ; Volume 29, Issue 6 , 2018 , Pages 683-700 ; 09205063 (ISSN) Sivandzade, F ; Mashayekhan, S ; Sharif University of Technology
    Taylor and Francis Inc  2018
    Cartilage is an avascular tissue with limited self-repair ability. Since the methods for treatment of cartilage defects have not been effective, new therapies based on tissue engineering are considered over the recent years. In this study, human cartilage tissue was decellularized and porous injectable microcarriers (MCs) composed of acellular extracellular matrix (ECM) of cartilage tissue and chitosan (CS), with different ECM weight ratios, were fabricated by electrospraying technique to be used in the treatment of articular cartilage defects. Various properties of ECM/CS MCs such as microstructure, mechanical strength, water uptake behaviour, and biodegradability rate were investigated.... 

    Stereolithography 3D bioprinting method for fabrication of human corneal stroma equivalent

    , Article Annals of Biomedical Engineering ; Volume 48, Issue 7 , June , 2020 , Pages 1955-1970 Mahdavi, S. S ; Abdekhodaie, M. J ; Kumar, H ; Mashayekhan, S ; Baradaran Rafii, A ; Kim, K ; Sharif University of Technology
    Springer  2020
    Abstract: 3D bioprinting technology is a promising approach for corneal stromal tissue regeneration. In this study, gelatin methacrylate (GelMA) mixed with corneal stromal cells was used as a bioink. The visible light-based stereolithography (SLA) 3D bioprinting method was utilized to print the anatomically similar dome-shaped structure of the human corneal stroma. Two different concentrations of GelMA macromer (7.5 and 12.5%) were tested for corneal stroma bioprinting. Due to high macromer concentrations, 12.5% GelMA was stiffer than 7.5% GelMA, which made it easier to handle. In terms of water content and optical transmittance of the bioprinted scaffolds, we observed that scaffold with... 

    Fabrication of biocompatible titanium scaffolds using space holder technique

    , Article Journal of Materials Science: Materials in Medicine ; Volume 23, Issue 10 , 2012 , Pages 2483-2488 ; 09574530 (ISSN) Dezfuli, S. N ; Sadrnezhaad, S. K ; Shokrgozar, M. A ; Bonakdar, S ; Sharif University of Technology
    Springer  2012
    Open-pore titanium scaffolds were fabricated by sintering of compressed mixtures of TiH1.924 and urea. Spherical and irregular shaped space holders were used to investigate the effect of pore shape on cellular behavior. After removal of the space holder, the shape of the spacers was replicated to the pores. Average diameter of the pores was in the range of 300-600 lm. SEM images showed that titanium hydride resulted in higher surface roughness and larger micro porosities than pure titanium. In vitro evaluationswere carried out by using MTT assay, measuring alkaline phosphatase activity and alizarin red staining in flow perfusion bioreactor for cell culture. Observations revealed excellent... 

    MicroRNA profiling reveals important functions of miR-125b and let-7a during human retinal pigment epithelial cell differentiation

    , Article Experimental Eye Research ; Volume 190 , 2020 Shahriari, F ; Satarian, L ; Moradi, S ; Sharifi Zarchi, A ; Günther, S ; Kamal, A ; Totonchi, M ; Mowla, S. J ; Braun, T ; Baharvand, H ; Sharif University of Technology
    Academic Press  2020
    Retinal pigment epithelial (RPE) cells are indispensable for eye organogenesis and vision. To realize the therapeutic potential of in vitro-generated RPE cells for cell-replacement therapy of RPE-related retinopathies, molecular mechanisms of RPE specification and maturation need to be investigated. So far, many attempts have been made to decipher the regulatory networks involved in the differentiation of human pluripotent stem cells into RPE cells. Here, we exploited a highly-efficient RPE differentiation protocol to determine global expression patterns of microRNAs (miRNAs) during human embryonic stem cell (hESC) differentiation into RPE using small RNA sequencing. Our results revealed a... 

    Defining microRNA signatures of hair follicular stem and progenitor cells in healthy and androgenic alopecia patients

    , Article Journal of Dermatological Science ; Volume 101, Issue 1 , 2021 , Pages 49-57 ; 09231811 (ISSN) Mohammadi, P ; Nilforoushzadeh, M. A ; Youssef, K. K ; Sharifi Zarchi, A ; Moradi, S ; Khosravani, P ; Aghdami, R ; Taheri, P ; Hosseini Salekdeh, G ; Baharvand, H ; Aghdami, N ; Sharif University of Technology
    Elsevier Ireland Ltd  2021
    Background: The exact pathogenic mechanism causes hair miniaturization during androgenic alopecia (AGA) has not been delineated. Recent evidence has shown a role for non-coding regulatory RNAs, such as microRNAs (miRNAs), in skin and hair disease. There is no reported information about the role of miRNAs in hair epithelial cells of AGA. Objectives: To investigate the roles of miRNAs affecting AGA in normal and patient's epithelial hair cells. Methods: Normal follicular stem and progenitor cells, as well as follicular patient's stem cells, were sorted from hair follicles, and a miRNA q-PCR profiling to compare the expression of 748 miRNA (miRs) in sorted cells were performed. Further, we...