Search for: human-mesenchymal-stem-cells
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    Graphene nanogrids for selective and fast osteogenic differentiation of human mesenchymal stem cells

    , Article Carbon ; Volume 59 , 2013 , Pages 200-211 ; 00086223 (ISSN) Akhavan, O ; Ghaderi, E ; Shahsavar, M ; Sharif University of Technology
    Graphene nanogrids (fabricated by graphene nanoribbons obtained through oxidative unzipping of multi-walled carbon nanotubes) were used as two-dimensional selective templates for accelerated differentiation of human mesenchymal stem cells (hMSCs), isolated from umbilical cord blood, into osteogenic lineage. The biocompatible and hydrophilic graphene nanogrids showed high actin cytoskeleton proliferations coinciding with patterns of the nanogrids. The amounts of proliferations were found slightly better than proliferation on hydrophilic graphene oxide (GO) sheets, and significantly higher than non-uniform proliferations on hydrophobic reduced graphene oxide (rGO) sheets and... 

    Genotoxicity of graphene nanoribbons in human mesenchymal stem cells

    , Article Carbon ; Volume 54 , 2013 , Pages 419-431 ; 00086223 (ISSN) Akhavan, O ; Ghaderi, E ; Emamy, H ; Akhavan, F ; Sharif University of Technology
    Single-layer reduced graphene oxide nanoribbons (rGONRs) were obtained through an oxidative unzipping of multi-walled carbon nanotubes and a subsequent deoxygenation by hydrazine and bovine serum albumin. Human mesenchymal stem cells (hMSCs) were isolated from umbilical cord blood and used for checking the concentration- and time-dependent cyto- and geno-toxic effects of the rGONRs and reduced graphene oxide sheets (rGOSs). The cell viability assay indicated significant cytotoxic effects of 10 μg/mL rGONRs after 1 h exposure time, while the rGOSs exhibited the same cytotoxicity at concentration of 100 μg/mL after 96 h. The oxidative stress was found as the main mechanism involved in the... 

    Combinatorial screening of nanoclay-reinforced hydrogels: a glimpse of the "holy grail" in orthopedic stem cell therapy?

    , Article ACS Applied Materials and Interfaces ; Volume 10, Issue 41 , 2018 , Pages 34924-34941 ; 19448244 (ISSN) Hasany, M ; Thakur, A ; Taebnia, N ; Kadumudi, F. B ; Shahbazi, M. A ; Pierchala, M. K ; Mohanty, S ; Orive, G ; Andresen, T. L ; Foldager, C. B ; Yaghmaei, S ; Arpanaei, A ; Gaharwar, A. K ; Mehrali, M ; Dolatshahi Pirouz, A ; Sharif University of Technology
    American Chemical Society  2018
    Despite the promise of hydrogel-based stem cell therapies in orthopedics, a significant need still exists for the development of injectable microenvironments capable of utilizing the regenerative potential of donor cells. Indeed, the quest for biomaterials that can direct stem cells into bone without the need of external factors has been the "Holy Grail" in orthopedic stem cell therapy for decades. To address this challenge, we have utilized a combinatorial approach to screen over 63 nanoengineered hydrogels made from alginate, hyaluronic acid, and two-dimensional nanoclays. Out of these combinations, we have identified a biomaterial that can promote osteogenesis in the absence of... 

    Enzymatically crosslinked hyaluronic acid microgels as a vehicle for sustained delivery of cationic proteins

    , Article European Polymer Journal ; Volume 115 , 2019 , Pages 234-243 ; 00143057 (ISSN) Jooybar, E ; Abdekhodaie, M. J ; Mousavi, A ; Zoetebier, B ; Dijkstra, P. J ; Sharif University of Technology
    Elsevier Ltd  2019
    In this study, a novel biodegradable hyaluronic acid (HA) based microgel were prepared via enzymatic crosslinking of tyramine conjugated HA (HA-TA) in an inverse microemulsion. HA-TA microdroplets were crosslinked within a few seconds in the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H 2 O 2 ). The high water content of the polymeric network and the inherent negative charge of the HA-TA microgels provided a suitable platform for encapsulation of cationic proteins like lysozyme and TGF-β1 growth factor. The results demonstrated that lysozyme was released, after an initial burst release, in a suitable sustained manner over a period of four weeks. Both diffusion and... 

    Injectable polyethylene glycol-laponite composite hydrogels as articular cartilage scaffolds with superior mechanical and rheological properties

    , Article International Journal of Polymeric Materials and Polymeric Biomaterials ; Volume 66, Issue 3 , 2017 , Pages 105-114 ; 00914037 (ISSN) Nojoomi, A ; Tamjid, E ; Simchi, A ; Bonakdar, S ; Stroeve, P ; Sharif University of Technology
    Taylor and Francis Inc  2017
    In this study, injectable PEG-based hydrogels containing Laponite particles with mechanical and structural properties close to the natural articular cartilage are introduced. The nanocomposites are fabricated by imide ring opening reactions utilizing synthesized copolymers containing PEG blocks and nanoclay through a two-step thermal poly-(amic acid) process. Butane diamine is used as nucleophilic reagent and hydrogels with interconnected pores with sizes in the range of 100–250 µm are prepared. Improved viscoelastic properties compared with the conventional PEG hydrogels are shown. Evaluation of cell viability utilizing human mesenchymal stem cells determines cytocompatibility of the... 

    Bioinspired nanofiber scaffold for differentiating bone marrow-derived neural stem cells to oligodendrocyte-like cells: Design, fabrication, and characterization

    , Article International Journal of Nanomedicine ; Volume 15 , 2020 , Pages 3903-3920 Boroojeni, F. R ; Mashayekhan, S ; Abbaszadeh, H. A ; Ansarizadeh, M ; Khoramgah, M. S ; Rahimi Movaghar, V ; Sharif University of Technology
    Dove Medical Press Ltd  2020
    Background: Researchers are trying to study the mechanism of neural stem cells (NSCs) differentiation to oligodendrocyte-like cells (OLCs) as well as to enhance the selective differentiation of NSCs to oligodendrocytes. However, the limitation in nerve tissue acces-sibility to isolate the NSCs as well as their differentiation toward oligodendrocytes is still challenging. Purpose: In the present study, a hybrid polycaprolactone (PCL)-gelatin nanofiber scaffold mimicking the native extracellular matrix and axon morphology to direct the differentiation of bone marrow-derived NSCs to OLCs was introduced. Materials and Methods: In order to achieve a sustained release of T3, this factor was... 

    The synergistic effect of surface topography and sustained release of TGF-β1 on myogenic differentiation of human mesenchymal stem cells

    , Article Journal of Biomedical Materials Research - Part A ; Volume 104, Issue 7 , 2016 , Pages 1610-1621 ; 15493296 (ISSN) Moghadasi Boroujeni, S ; Mashayekhan, S ; Vakilian, S ; Ardeshirylajimi, A ; Soleimani, M ; Sharif University of Technology
    John Wiley and Sons Inc 
    A combination of topographical cues and controlled release of biochemical factors is a potential platform in controlling stem cells differentiation. In this study the synergistic effect of nanotopography and sustained release of biofunctional transforming growth factor beta 1 (TGF-β1) on differentiation of human Wharton's Jelly-derived mesenchymal stem cell (hWJ-derived UC-MSCs) toward myogenic lineage was investigated. In order to achieve a sustained release of TGF-β1, this factor was encapsulated within chitosan nanoparticles. Afterwards the aligned composite mats were fabricated using poly-E-caprolacton (PCL) containing TGF-β1-loaded chitosan nanoparticles and poly-L-lactic acid (PLLA).... 

    Silica nanoparticle surface chemistry: An important trait affecting cellular biocompatibility in two and three dimensional culture systems

    , Article Colloids and Surfaces B: Biointerfaces ; Volume 182 , 2019 ; 09277765 (ISSN) Hasany, M ; Taebnia, N ; Yaghmaei, S ; Shahbazi, M. A ; Mehrali, M ; Dolatshahi Pirouz, A ; Arpanaei, A ; Sharif University of Technology
    Elsevier B.V  2019
    Great advantages bestowed by mesoporous silica nanoparticles (MSNs) including high surface area, tailorable pore diameter and surface chemistry, and large pore volume render them as efficient tools in biomedical applications. Herein, MSNs with different surface chemistries were synthesized and investigated in terms of biocompatibility and their impact on the morphology of bone marrow-derived mesenchymal stem cells both in 2D and 3D culture systems. Bare MSNs (BMSNs) were synthesized by template removing method using tetraethylorthosilicate (TEOS) as a precursor. The as-prepared BMSNs were then used to prepare amine-functionalized (AMSNs), carboxyl-functionalized (CMSNs) and polymeric... 

    Size-dependent genotoxicity of graphene nanoplatelets in human stem cells

    , Article Biomaterials ; Volume 33, Issue 32 , 2012 , Pages 8017-8025 ; 01429612 (ISSN) Akhavan, O ; Ghaderi, E ; Akhavan, A ; Sharif University of Technology
    Reduced graphene oxide nanoplatelets (rGONPs) were synthesized by sonication of covalently PEGylated GO sheets followed by a chemical reduction using hydrazine and bovine serum albumin. Human mesenchymal stem cells (hMSCs), as a fundamental factor in tissue engineering, were isolated from umbilical cord blood (as a recently proposed source for extracting fresh hMSCs) to investigate, for the first time, the size-dependent cyto- and geno-toxic effects of the rGONPs on the cells. The cell viability test showed significant cell destructions by 1.0 μg/mL rGONPs with average lateral dimensions (ALDs) of 11±4 nm, while the rGO sheets with ALDs of 3.8±0.4 μm could exhibit a significant cytotoxic...