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Dosign and Construction of Natural Polymers-based Hydrogels for Cartilage Tissue Engineering
, M.Sc. Thesis Sharif University of Technology ; Mashayekhan, Shohreh (Supervisor) ; Hajebrahimi, Zahra (Supervisor)
Abstract
Once damaged, articular cartilage has very little capacity for spontaneous healing because of the avascular nature of the tissue. Although many repair techniques have been proposed over the past decades, none has successfully regenerated long-lasting tissue to replace damaged cartilage. Tissue engineering have recently demonstrated tremendous approaches for regeneration of cartilage tissue lesions. Tissue engineering is based on three principles: cells, scaffolds for cell adhesion and growth factors. Three-dimensional biodegradable scaffolds play an important role in tissue engineering. In this study, novel cross-linked hybrid chitosan/ECM scaffolds were prepared for articular cartilage...
Optimization of Insulin Release from Beta Cells Using Hybrid Scaffold Bioengineering
, M.Sc. Thesis Sharif University of Technology ; Alemzadeh, Iran (Supervisor) ; Vossoughi, Manouchehr (Supervisor)
Abstract
Combined composites consisting of nanofibers and hydrogels were fabricated to grow cells in an environment that mimicked the extracellular matrix. Electrospan nanofibers were spun and hydrolyzed under optimized conditions consisting of polycaprolactone (PCL) and gelatin and then incorporated into alginate and gelatin hydrogels. Nanofibril-containing (NF / hydrogel) hydrogel hybrids were ligated by calcium chloride in the presence of encapsulated beta cells. The mechanical properties of NF / hydrogel scaffolds were significantly increased in proportion to the NF content in NF / hydrogels. Beta cells killed in NF / hydrogels showed higher adhesion to the matrix compared to samples without NF....
Mechanical reinforcement of urinary bladder matrix by electrospun polycaprolactone nanofibers
, Article Scientia Iranica ; Volume 24, Issue 6 , 2017 , Pages 3476-3480 ; 10263098 (ISSN) ; Rajabi Zeleti, S ; Naji, M ; Ghanian, M. H ; Baharvand, H ; Sharif University of Technology
Abstract
For a successful repair and reconstruction of bladder tissue, fabrication of scaffolds with proper biochemical and biomechanical characteristics is necessary. Decellularized bladder tissue has been proposed in previous studies as a gold standard material for scaffold fabrication. However, weak mechanical properties of such a load-bearing tissue has remained a challenge. Incorporation of both biological and synthetic materials has been known as an effective strategy for improving mechanical and biological properties of the scaffolds. In the present work, a simple process was developed to fabricate hybrid hydrogel scaffolds with a biomimetic architecture from the natural urinary bladder...
ZnO-incorporated polyvinylidene fluoride/poly(ε-caprolactone) nanocomposite scaffold with controlled release of dexamethasone for bone tissue engineering
, Article Applied Physics A: Materials Science and Processing ; Volume 128, Issue 8 , 2022 ; 09478396 (ISSN) ; Mohammadi, M ; Mashayekhan, S ; Sharif University of Technology
Springer Science and Business Media Deutschland GmbH
2022
Abstract
Here we report on the development of a hybrid nanofibrous scaffold made from polyvinylidene fluoride (PVDF) nanofibers embedding zinc oxide nanorods (ZnOns), and poly(ε-caprolactone) (PCL) nanofibers incorporating dexamethasone (DEX)-loaded chitosan nanoparticles using dual-electrospinning method. Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), scanning electron microscopy (SEM), and tensile analysis were carried out for physiochemical characterization of the scaffolds, followed by DEX release profile. In addition, an MTT assay was conducted to assess the viability of mouse bone marrow-derived mesenchymal stem cells (mBMSCs) on the hybrid nanofibrous scaffold....
A hybrid scaffold of gelatin glycosaminoglycan matrix and fibrin as a carrier of human corneal fibroblast cells
, Article Materials Science and Engineering C ; Volume 118 , 2021 ; 09284931 (ISSN) ; Mahdavi salimi, S ; Abdekhodaie, M. J ; Baradaran Rafii, A ; Tabatabei, M. R ; Mehrvar, M ; Sharif University of Technology
Elsevier Ltd
2021
Abstract
A hybrid scaffold of gelatin-glycosaminoglycan matrix and fibrin (FGG) has been synthesized to improve the mechanical properties, degradation time and cell response of fibrin-like scaffolds. The FGG scaffold was fabricated by optimizing some properties of fibrin-only gel and gelatin-glycosaminoglycan (GG) scaffolds. Mechanical analysis of optimized fibrin-only gel showed the Young module and tensile strength of up to 72 and 121 KPa, respectively. Significantly, the nine-fold increase in the Young modulus and a seven-fold increase in tensile strength was observed when fibrin reinforced with GG scaffold. Additionally, the results demonstrated that the degradation time of fibrin was enhanced...
Fabrication of porous scaffolds with decellularized cartilage matrix for tissue engineering application
, Article Biologicals ; Volume 48 , 2017 , Pages 39-46 ; 10451056 (ISSN) ; Mashayekhan, S ; Sharif University of Technology
Academic Press
2017
Abstract
Due to the avascular nature of articular cartilage, damaged tissue has little capacity for spontaneous healing. Three-dimensional scaffolds have potential for use in tissue engineering approach for cartilage repair. In this study, bovine cartilage tissue was decellularized and chemically crosslinked hybrid chitosan/extracellular matrix (ECM) scaffolds were fabricated with different ECM weight ratios by simple freeze drying method. Various properties of chitosan/ECM scaffolds such as microstructure, mechanical strength, swelling ratio, and biodegradability rate were investigated to confirm improved structural and biological characteristics of chitosan scaffolds in the presence of ECM. The...