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    Electrospun polyethersolfone nanofibrous membrane as novel platform for protein immobilization in microfluidic systems

    , Article Journal of Biomedical Materials Research - Part B Applied Biomaterials ; Volume 106, Issue 3 , April , 2018 , Pages 1108-1120 ; 15524973 (ISSN) Mahmoudifard, M ; Vossoughi, M ; Soudi, S ; Soleimani, M ; Sharif University of Technology
    John Wiley and Sons Inc  2018
    In the present study, the feasibility of electrospun polyethersolfone (PES) nanofibrous membrane as the solid substrate for microfluidic based immunoassays to enhance the density of immobilized antibody on the surface of membrane was assessed. Conversely, the efficacy of antibody immobilization was compared by two different strategies as 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) coupling chemistry and hydrophobic interaction. Compared to conventional immunoassays carried out in plates or gels, microfluidic based immunoassays grant a lot of advantages such as a consumption of little samples and reagents, shorter analysis time, and higher efficiency.... 

    Efficient protein immobilization on polyethersolfone electrospun nanofibrous membrane via covalent binding for biosensing applications

    , Article Materials Science and Engineering C ; Volume 58 , 2016 , Pages 586-594 ; 09284931 (ISSN) Mahmoudifard, M ; Soudi, S ; Soleimani, M ; Hosseinzadeh, S ; Esmaeili, E ; Vossoughi, M ; Sharif University of Technology
    Elsevier Ltd  2016
    In this paper we introduce novel strategy for antibody immobilization using high surface area electrospun nanofibrous membrane based on ethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-hydroxysuccinimide (EDC/NHS) coupling chemistry. To present the high performance of proposed biosensors, anti-staphylococcus enterotoxin B (anti-SEB) was used as a model to demonstrate the utility of our proposed system. Polymer solution of polyethersolfone was used to fabricate fine nanofibrous membrane. Moreover, industrial polyvinylidene fluoride membrane and conventional microtiter plate were also used to compare the efficiency of antibody immobilization. Scanning electron microscopy images were taken to... 

    ZnO nanoparticle/nanorod-based label-free electrochemical immunoassay for rapid detection of MMP-9 biomarker

    , Article Biochemical Engineering Journal ; Volume 164 , 2020 Shabani, E ; Abdekhodaie, M. J ; Mousavi, S. A ; Taghipour, F ; Sharif University of Technology
    Elsevier B.V  2020
    A label-free electrochemical biosensor was developed for the rapid detection of the matrix metalloproteinase 9 (MMP-9) biomarker on the basis of antibody immobilizing on the zinc oxide (ZnO) nanoparticle and ZnO nanorod electrodes. The charge transfer resistance (Rct) of the electrodes was used as the indicator for MMP-9 concentration, which was obtained through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The ZnO nanorod-based biosensor exhibited linear behavior in the MMP-9 concentration range of 1–1000 ng/ml, which is a wider range than the available concentration ranges for most of the conventional methods. The biosensor sensitivity was 32.5 μA/(decade × cm2)... 

    Design of an effective piezoelectric microcantilever biosensor for rapid detection of COVID-19

    , Article Journal of Medical Engineering and Technology ; Volume 45, Issue 6 , 2021 , Pages 423-433 ; 03091902 (ISSN) Kabir, H ; Merati, M ; Abdekhodaie, M. J ; Sharif University of Technology
    Taylor and Francis Ltd  2021
    Acute respiratory syndrome coronavirus 2 (SARS-CoV-2), also called COVID-19, is one of the most contagious viruses resulting in a progressive pandemic. Since specific antiviral treatments have not been developed yet and its fatal rate is almost high, early and fast detection is critical for controlling the outbreak. In this study, a piezoelectric microcantilever biosensor has been designed for detecting COVID-19 samples directly without requiring preparation steps. The biosensor acts as a transducer and is coated with the related antibody. When the SARS-CoV-2 antigens adsorbed on the microcantilever top surface through their spike proteins, a surface stress due to the mass change would be...