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immobilized-horseradish-peroxidase
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Phenols removal by immobilized horseradish peroxidase
, Article Journal of Hazardous Materials ; Volume 166, Issue 2-3 , 2009 , Pages 1082-1086 ; 03043894 (ISSN) ; Nejati, S ; Sharif University of Technology
2009
Abstract
Application of immobilized horseradish peroxidase (HRP) in porous calcium alginate (ca-alginate) for the purpose of phenol removal is reported. The optimal conditions for immobilization of HRP in ca-alginate were identified. Gelation (encapsulation) was optimized at 1.0% (w/v) sodium alginate in the presence of 5.5% (w/v) of calcium chloride. Upon immobilization, pH profile of enzyme activity changes as it shows higher value at basic and acidic solution. Increasing initial phenol concentration results in a decrease in % conversion. The highest conversion belongs to phenol concentration of 2 mM. Investigation into time course of phenol removal for both encapsulated and free enzymes showed...