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    Electrospun polyethersolfone nanofibrous membrane as novel platform for protein immobilization in microfluidic systems

    , Article Journal of Biomedical Materials Research - Part B Applied Biomaterials ; 2017 ; 15524973 (ISSN) Mahmoudifard, M ; Vossoughi, M ; Soudi, S ; Soleimani, M ; Sharif University of Technology
    John Wiley and Sons Inc  2017
    Abstract
    In the present study, the feasibility of electrospun polyethersolfone (PES) nanofibrous membrane as the solid substrate for microfluidic based immunoassays to enhance the density of immobilized antibody on the surface of membrane was assessed. Conversely, the efficacy of antibody immobilization was compared by two different strategies as 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) coupling chemistry and hydrophobic interaction. Compared to conventional immunoassays carried out in plates or gels, microfluidic based immunoassays grant a lot of advantages such as a consumption of little samples and reagents, shorter analysis time, and higher efficiency.... 

    Different types of electrospun nanofibers and their effect on microfluidic-based immunoassay

    , Article Polymers for Advanced Technologies ; Volume 30, Issue 4 , 2019 , Pages 973-982 ; 10427147 (ISSN) Mahmoudifard, M ; Vossoughi, M ; Soleimani, M ; Sharif University of Technology
    John Wiley and Sons Ltd  2019
    Abstract
    Protein capturing on polymeric substrate of microfluidic devices is a key factor for the fabrication of immunoassay with high sensitivity. In this work, simple and versatile technique of electrospinning was used to produce electrospun nanofibrous membranes (e.NFMs) with high surface area as a substrate for microfluidic-based immunoassay to increase sensitivity. It was found that the simultaneous use of e.NFM and 1-Ethylethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-Hydroxysuccinimide hydroxysuccinimide as coupling agent has synergic effect on antigen immobilization onto the microchannels. It was found that the oxygen plasma technique for the creation of oxygen containing functional group... 

    Antibody consumption reduction in lateral flow immunoassays within porous media

    , Article Chemical Engineering and Processing - Process Intensification ; Volume 147 , 2020 Gholami, A ; Shamloo, A ; Sharif University of Technology
    Elsevier B.V  2020
    Abstract
    In this study, the effect of the geometrical parameters of the Lateral Flow Immunoassay (LFI) membrane on the performance of the pregnancy kits is investigated. Consequently, a new geometry for LFI membrane is proposed based on some theoretical, numerical and experimental observations in order to improve the performance of the related kits. A pregnancy kit (for the detection of hCG in urine samples) is developed and the effect of the relocation of the test and the control lines over the membrane was studied based on the variation of the sample fluid velocity. Using Lattice-Boltzmann simulation of the lateral flow within the porous media and the experimental results, the fluid flow within the... 

    Design and Manufacturing of an Assay System by Lateral Flow Method and Optimize the Model by Lattice-Boltzmann Numerical Solution

    , M.Sc. Thesis Sharif University of Technology Gholami Poshtehani, Abbas (Author) ; Shamloo, Amir (Supervisor)
    Abstract
    Lateral Flow Immunoassay kits are one of the most important instrument in laboratories and houses for diagnosing diseases, hormones, food toxins and etc. fast, exactly and by low cost. Since one of the most expensive parts in the mentioned kits, is using antibodies in their construction, an attempt has been made in this project for descending usage of antibody and consequently final cost of these sensors besides keeping desired and necessary sensitivity of these diagnostic kits. For this purpose, first of all with the help of simulating flow’s behavior in Lattice-Boltzmann method, provisions has been made to predicting flow’s movement behavior in Nitrocellulose membrane, Based on its results... 

    Design and Fabrication of Microfluidic Biosensors based on Modified Electrospun Nanofibrous Membranes for Biomarkers Detection

    , Ph.D. Dissertation Sharif University of Technology Mahmoudifard, Matin (Author) ; Vosoughi, Manuchehr (Supervisor) ; Soleimani, Masoud (Supervisor) ; Soudi, Sara (Supervisor)
    Abstract
    The accurate assessment of biomarkers likes proteins, hormones or other biomolecules that amount of them changes with state of disease or level of treatment and gives diagnostic information is a difficult duty. In these samples, pathogens exist in small quantity compared to other background molecules. Therefore, appropriate sampling process for concentrating and separating pathogens from the matrix should be developed in order to provide rapid and accurate pathogen detection test. In this thesis design and fabrication of microfluidic biosensors based on the modified nanofibrous membranes were investigated and explained. PES nanofibrous membrane with carboxyl functional groups for covalent... 

    Designing and Fabrication of Microfluidic Biosensor by DNA-Directed Immobilization

    , Ph.D. Dissertation Sharif University of Technology Esmaeili, Elaheh (Author) ; Vossoughi, Manouchehr (Supervisor) ; Soleimani, Masoud (Supervisor) ; Shamloo, Amir (Supervisor)
    Abstract
    The present study is aimed at the development of a novel approach based on the magnetically improvement of DNA-directed immobilization to prepare a highly efficient sensor for prostate diagnosis. The novelty of this work is in the use of antibody conjugated magnetic nanoparticles via DDI. DNA-modified magnetic nanoparticles are added in solution to capture DNA-conjugated, fluorescently-labeled immunocomplexes formed in solution free of steric constraints. The DDI-based nanoconstructs are then concentrated and immobilized using a magnetic field. Compared to a process in which the immunocomplex directly forms on the sensing surface, the proposed approach provides higher mass transfer and lower... 

    The set-up of a high temperature superconductor radio-frequency SQUID microscope for magnetic nanoparticle detection

    , Article Superconductor Science and Technology ; Volume 19, Issue 5 , 2006 , Pages S261-S265 ; 09532048 (ISSN) Schmidt, M ; Krause, H. J ; Banzet, M ; Lomparski, D ; Schubert, J ; Zander, W ; Zhang, Y ; Akram, R ; Fardmanesh, M ; Sharif University of Technology
    2006
    Abstract
    SQUID (superconducting quantum interference device) microscopes are versatile instruments for biosensing applications, in particular for magnetic nanoparticle detection in immunoassay experiments. We are developing a SQUID microscope based on an HTS rf SQUID magnetometer sensor with a substrate resonator. For the cryogenic set-up, a configuration was realized in which the cryostat is continuously refilled and kept at a constant liquid nitrogen level by an isolated tube connection to a large liquid nitrogen reservoir. The SQUID is mounted on top of a sapphire finger, connected to the inner vessel of the stainless steel cryostat. The vacuum gap between the cold SQUID and room temperature... 

    Effective surface modification of MnFe2O4@SiO2@PMIDA magnetic nanoparticles for rapid and high-density antibody immobilization

    , Article Applied Surface Science ; Volume 426 , 2017 , Pages 1023-1029 ; 01694332 (ISSN) Rashid, Z ; Soleimani, M ; Ghahremanzadeh, R ; Vossoughi, M ; Esmaeili, E ; Sharif University of Technology
    Abstract
    The present study is aimed at the synthesis of MnFe2O4@SiO2@PMIDA in terms of highly efficient sensing platform for anti-prostate specific membrane antigen (PSMA) immobilization. Superparamagnetic manganese ferrite nanoparticles were synthesized following co-precipitation method and then SiO2 shell was coated on the magnetic core with tetraethyl orthosilicate (TEOS) through a silanization reaction to prevent oxidation, agglomeration and, increase the density of OH groups on the surface of MnFe2O4. Subsequently, MnFe2O4@SiO2@PMIDA obtained as a result of the reaction between N-(phosphonomethyl)iminodiacetic acid (PMIDA) and MnFe2O4@SiO2. The reactive carboxyl groups on the surface of magnetic... 

    Development of a nano biosensor for anti-gliadin detection for Celiac disease based on suspension microarrays

    , Article Biomedical Physics and Engineering Express ; Volume 6, Issue 5 , August , 2020 Kharati, M ; Rabiee, M ; Rostami Nejad, M ; Aghamohammadi, E ; Asadzadeh Aghdaei, H ; Zali, M. R ; Rabiee, N ; Fatahi, Y ; Bagherzadeh, M ; Webster, T. J ; Sharif University of Technology
    IOP Publishing Ltd  2020
    Abstract
    Celiac disease is an autoimmune disorder represented by the ingestion of the gluten protein usually found in wheat, barley and rye. To date, ELISA has been the most accurate method for determining the presence of anti-gliadin, which is cumbersome, expensive (compared to a suspension microarray technique), and requires extensive sample preparation. In this study, in order to establish a more accurate assay to identify gliadin at lower concentrations, optical nano biosensors using an indirect immunoassay method for gliadin detection was designed and fabricated. For this, polycaprolactone (PCL) nano- to micro-beads were fabricated as a platform for the gliadin antigen which were optimized and... 

    Development of Ratiometric Sensors Based on Fluorescent Nanostructures for Naked Eye Detection

    , Ph.D. Dissertation Sharif University of Technology Farahmand Nejad, Mohammad Amin (Author) ; Hormozi-Nezhad, Mohammad Reza (Supervisor)
    Abstract
    In the first part of this research, we have developed a simple and effective ratiometric fluorescence sensor for selective detection of dopamine (DA) in alkaline media by simply mixing thioglycolic acid (TGA) functionalized orange fluorescent cadmium telluride (CdTe) quantum dots (QDs) with amino-functionalized blue fluorescent carbon nanodots (CDs). Under a single excitation wavelength of 365 nm, the sensor exhibits dual-emissions centered at 445 and 603 nm. The fluorescence of CdTe QDs is selectively quenched by DA, whereas the fluorescence of CDs is insensitive to the analyte. In the presence of different amounts of DA, the variations in the dual emission intensity ratios exhibit a... 

    Effect of mozart music on hippocampal content of BDNF in postnatal rats

    , Article Basic and Clinical Neuroscience ; Volume 2, Issue 3 , 2011 , Pages 21-26 ; 2008126X (ISSN) Marzban, M ; Shahbazi, A ; Tondar, M ; Soleimani, M ; Bakhshayesh, M ; Moshkforoush, A ; Sadati, M ; Zendehrood, S. A ; Joghataei, M. T ; Sharif University of Technology
    2011
    Abstract
    Introduction: It has shown that listening to Mozart music can potentiate spatial tasks in human; and reduce seizure attacks in epileptic patients. A few studies have reported the effects of prenatal plus postpartum exposure of mice to the Mozart music on brain-drived neurotrophic factor (BDNF) in the hippocampus. Here we investigated the effect of postpartum exposure to The Mozart music on BDNF concentration in the hippocampus of rat. Methods: Thirty male one day old newborn Wistar rats divided randomly in two equal experimental and control groups. Experimental group exposed to slow rhythm Mozart music (Mozart Sonata for two pianos KV 448, 6 hour per day; sound pressure levels, between 80... 

    A novel ratiometric fluorescent approach for the modulation of the dynamic range of lateral flow immunoassays

    , Article Advanced Materials Technologies ; Volume 7, Issue 8 , 2022 ; 2365709X (ISSN) Sena-Torralba, A ; Torné Morató, H ; Parolo, C ; Ranjbar, S ; Farahmand Nejad, M. A ; Álvarez Diduk, R ; Idili, A ; Hormozi Nezhad, M. R ; Merkoçi, A ; Sharif University of Technology
    John Wiley and Sons Inc  2022
    Abstract
    The majority of lateral flow assays (LFAs) use single-color optical labels to provide a qualitative naked-eye detection, however this detection method displays two important limitations. First, the use of a single-color label makes the LFA prone to results misinterpretation. Second, it does not allow the precise modulation of the sensitivity and dynamic range of the test. To overcome these limitations, a ratiometric approach is developed. In particular, using anti-HIgG functionalized red-fluorescent quantum dots on the conjugate pad (as target dependent labels) and blue-fluorescent nanoparticles fixed on the test line (as target independent reporters), it is possible to generate a wide color... 

    Electrospun polyethersolfone nanofibrous membrane as novel platform for protein immobilization in microfluidic systems

    , Article Journal of Biomedical Materials Research - Part B Applied Biomaterials ; Volume 106, Issue 3 , April , 2018 , Pages 1108-1120 ; 15524973 (ISSN) Mahmoudifard, M ; Vossoughi, M ; Soudi, S ; Soleimani, M ; Sharif University of Technology
    John Wiley and Sons Inc  2018
    Abstract
    In the present study, the feasibility of electrospun polyethersolfone (PES) nanofibrous membrane as the solid substrate for microfluidic based immunoassays to enhance the density of immobilized antibody on the surface of membrane was assessed. Conversely, the efficacy of antibody immobilization was compared by two different strategies as 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) coupling chemistry and hydrophobic interaction. Compared to conventional immunoassays carried out in plates or gels, microfluidic based immunoassays grant a lot of advantages such as a consumption of little samples and reagents, shorter analysis time, and higher efficiency.... 

    ZnO nanoparticle/nanorod-based label-free electrochemical immunoassay for rapid detection of MMP-9 biomarker

    , Article Biochemical Engineering Journal ; Volume 164 , 2020 Shabani, E ; Abdekhodaie, M. J ; Mousavi, S. A ; Taghipour, F ; Sharif University of Technology
    Elsevier B.V  2020
    Abstract
    A label-free electrochemical biosensor was developed for the rapid detection of the matrix metalloproteinase 9 (MMP-9) biomarker on the basis of antibody immobilizing on the zinc oxide (ZnO) nanoparticle and ZnO nanorod electrodes. The charge transfer resistance (Rct) of the electrodes was used as the indicator for MMP-9 concentration, which was obtained through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The ZnO nanorod-based biosensor exhibited linear behavior in the MMP-9 concentration range of 1–1000 ng/ml, which is a wider range than the available concentration ranges for most of the conventional methods. The biosensor sensitivity was 32.5 μA/(decade × cm2)... 

    Possible role for growth hormone in suppressing acylated ghrelin and hunger ratings during and after intermittent exercise of different intensities in obese individuals

    , Article Acta Medica Iranica ; Vol. 52, Issue. 1 , 2014 , pp. 29-37 ; ISSN: 1735-9694 Gholipour, M ; Kordi, M. R ; Taghikhani, M ; Ravasi, A. A ; Gaeini, A. A ; Tabrizi, A ; Sharif University of Technology
    Abstract
    Body weight is influenced by both food intake and energy expenditure. Acylated ghrelin enhances appetite, and its circulating level is suppressed by Growth Hormone. Data on the acylated ghrelin responses to exercise of different intensities in obese individuals are currently not available. This study examined the effects of an intermittent exercise protocol on acylated ghrelin levels and hunger ratings in obese people. Nine inactive male ran on the treadmill at 0900 with progressive intensities of 50, 60, 70, and 80% of VO2max for 10, 10, 5, and 2 min respectively. Blood samples were collected before the exercise at 0845 (-15 min as the resting values), after each workload (10, 23, 31, and... 

    Design and fabrication of a centrifugal microfluidic disc including septum valve for measuring hemoglobin A1c in human whole blood using immunoturbidimetry method

    , Article Talanta ; Volume 190 , 2018 , Pages 134-139 ; 00399140 (ISSN) Mahmodi Arjmand, E ; Saadatmand, M ; Bakhtiari, M. R ; Eghbal, M ; Sharif University of Technology
    Elsevier B.V  2018
    Abstract
    Diabetes mellitus is a global endemic with a rapidly increasing prevalence in both developing and developed countries. Recently, hemoglobin A1c has been recommended by the American Diabetes Associations as a possible substitute for fasting blood glucose for the diagnosis of diabetes, because it is an indicator of long-term glycemic control. Also, centrifugal microfluidic systems have good potential for use in the point of care testing systems. In this study, a centrifugal microfluidic disc was designed and manufactured to measure hemoglobin A1c in whole blood using an immunoturbidimetry based method. Also, a new passive valve, named septum valve, was presented to precisely control the entry... 

    Label-Free real-time detection of HBsAg using a QCM immunosensor

    , Article Clinical Laboratory ; Volume 68, Issue 4 , 2022 , Pages 707-720 ; 14336510 (ISSN) Saffari, Z ; Ghavidel, A ; Ahangari Cohan, R ; Hamidi Fard, M ; Khoobi, M ; Aghasadeghi, M ; Norouzian, D ; Sharif University of Technology
    Verlag Klinisches Labor GmbH  2022
    Abstract
    Background: Hepatitis B virus surface antigen (HBsAg) is an important protein in both diagnosis and prevention of hepatitis B infection. In the current study, a piezoelectric immunosensor based on antibody-antigen interaction was designed to detect HBsAg. A quartz crystal microbalance system was employed to detect antibody-antigen interaction. Methods: At first, an oscillator was designed to measure the resonant frequency affected by the reactants using IC 74LVC1GX04. Antibody against HBsAg was immobilized on 10 MHz AT-cut quartz crystal. The surface modifications were monitored by atomic force microscopy (AFM) and contact angle measurements. Different concentrations of antibody were used... 

    Microfluidic devices with gold thin film channels for chemical and biomedical applications: a review

    , Article Biomedical Microdevices ; Volume 21, Issue 4 , 2019 ; 13872176 (ISSN) Ghasemi Toudeshkchoui, M ; Rabiee, N ; Rabiee, M ; Bagherzadeh, M ; Tahriri, M ; Tayebi, L ; Hamblin, M. R ; Sharif University of Technology
    Springer New York LLC  2019
    Abstract
    Microfluidic systems (MFS) provide a range of advantages in biomedical applications, including improved controllability of material characteristics and lower consumption of reagents, energy, time and money. Fabrication of MFS employs various materials, such as glass, silicon, ceramics, paper, and metals such as gold, copper, aluminum, chromium and titanium. In this review, gold thin film microfluidic channels (GTFMFC) are discussed with reference to fabrication methods and their diverse use in chemical and biomedical applications. The advantages of gold thin films (GTF) include flexibility, ease of manufacture, adhesion to polymer surfaces, chemical stability, good electrical conductivity,... 

    Green chemistry and coronavirus

    , Article Sustainable Chemistry and Pharmacy ; Volume 21 , 2021 ; 23525541 (ISSN) Ahmadi, S ; Rabiee, N ; Fatahi, Y ; Hooshmand, S. E ; Bagherzadeh, M ; Rabiee, M ; Jajarmi, V ; Dinarvand, R ; Habibzadeh, S ; Saeb, M. R ; Varma, R. S ; Shokouhimehr, M ; Hamblin, M. R ; Sharif University of Technology
    Elsevier B.V  2021
    Abstract
    The novel coronavirus pandemic has rapidly spread around the world since December 2019. Various techniques have been applied in identification of SARS-CoV-2 or COVID-19 infection including computed tomography imaging, whole genome sequencing, and molecular methods such as reverse transcription polymerase chain reaction (RT-PCR). This review article discusses the diagnostic methods currently being deployed for the SARS-CoV-2 identification including optical biosensors and point-of-care diagnostics that are on the horizon. These innovative technologies may provide a more accurate, sensitive and rapid diagnosis of SARS-CoV-2 to manage the present novel coronavirus outbreak, and could be... 

    Green chemistry and coronavirus

    , Article Sustainable Chemistry and Pharmacy ; Volume 21 , 2021 ; 23525541 (ISSN) Ahmadi, S ; Rabiee, N ; Fatahi, Y ; Hooshmand, S. E ; Bagherzadeh, M ; Rabiee, M ; Jajarmi, V ; Dinarvand, R ; Habibzadeh, S ; Saeb, M. R ; Varma, R.S ; Shokouhimehr, M ; Hamblin, M. R ; Sharif University of Technology
    Elsevier B.V  2021
    Abstract
    The novel coronavirus pandemic has rapidly spread around the world since December 2019. Various techniques have been applied in identification of SARS-CoV-2 or COVID-19 infection including computed tomography imaging, whole genome sequencing, and molecular methods such as reverse transcription polymerase chain reaction (RT-PCR). This review article discusses the diagnostic methods currently being deployed for the SARS-CoV-2 identification including optical biosensors and point-of-care diagnostics that are on the horizon. These innovative technologies may provide a more accurate, sensitive and rapid diagnosis of SARS-CoV-2 to manage the present novel coronavirus outbreak, and could be...