Search for: immunology
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    Different types of electrospun nanofibers and their effect on microfluidic-based immunoassay

    , Article Polymers for Advanced Technologies ; Volume 30, Issue 4 , 2019 , Pages 973-982 ; 10427147 (ISSN) Mahmoudifard, M ; Vossoughi, M ; Soleimani, M ; Sharif University of Technology
    John Wiley and Sons Ltd  2019
    Protein capturing on polymeric substrate of microfluidic devices is a key factor for the fabrication of immunoassay with high sensitivity. In this work, simple and versatile technique of electrospinning was used to produce electrospun nanofibrous membranes (e.NFMs) with high surface area as a substrate for microfluidic-based immunoassay to increase sensitivity. It was found that the simultaneous use of e.NFM and 1-Ethylethyl-3-(3-dimethylaminopropyl)-carbodiimide/N-Hydroxysuccinimide hydroxysuccinimide as coupling agent has synergic effect on antigen immobilization onto the microchannels. It was found that the oxygen plasma technique for the creation of oxygen containing functional group... 

    The effect of conventional immunosuppressive therapy on cytokine serum levels in pemphigus vulgaris patients

    , Article Iranian Journal of Allergy, Asthma and Immunology ; Vol. 13, issue. 3 , June , 2014 , p. 174-183 ; 17351502 Mortazavi, H ; Esmaili, N ; Khezri, S ; Khamesipour, A ; Farahani, I. V ; Daneshpazhooh, M ; Rezaei N ; Sharif University of Technology
    Pemphigus vulgaris is an autoimmune disease, in which the role of Th17 cytokines needs to be further explored. This study was performed to assess serum levels of three interleukins (IL) required for Th17 differentiation (IL-β, IL-6 and IL-23) and two specific Th17 cytokines (IL- 17 and IL-22) in a group of patients with pemphigus vulgaris, at baseline, 3 weeks and 6 months after of treatment. Correlations between anti-desmogleins and cytokines with disease severity as well as the influence of therapy on the above factors were assessed. Forty-three first-admitted pemphigus vulgaris patients with the active disease entered the study, but only 31 completed the study. Forty-five healthy... 

    Applying sequence alignment in tracking evolving clusters of web-sessions data: An artificial immune network approach

    , Article Proceedings - 3rd International Conference on Computational Intelligence, Communication Systems and Networks, CICSyN 2011, 26 July 2011 through 28 July 2011, Bali ; 2011 , Pages 42-47 ; 9780769544823 (ISBN) Azimpour Kivi, M ; Azmi, R ; Sharif University of Technology
    Artificial Immune System (AIS) models have outstanding properties, such as learning, adaptivity and robustness, which make them suitable for learning in dynamic and noisy environments such as the web. In this study, we tend to apply AIS for tracking evolving patterns of web usage data. The definition of the similarity of web sessions has an important impact on the quality of discovered patterns. Many prevalent web usage mining approaches ignore the sequential nature of web navigations for defining similarity between sessions. We propose the use of a new web sessions' similarity measure for investigating the usage data from web access log files. In this similarity measure, in addition to the... 

    Serological assays and host antibody detection in coronavirus-related disease diagnosis

    , Article Archives of Virology ; Volume 166, Issue 3 , 2021 , Pages 715-731 ; 03048608 (ISSN) Dowlatshahi, S ; Shabani, E ; Abdekhodaie, M. J ; Sharif University of Technology
    Springer  2021
    Coronaviruses (CoV) are a family of viral pathogens that infect both birds and mammals, including humans. Seven human coronaviruses (HCoV) have been recognized so far. HCoV-229E, -OC43, -NL63, and -HKU1 account for one-third of common colds with mild symptoms. The other three members are severe acute respiratory syndrome (SARS)-CoV, Middle East respiratory syndrome (MERS)-CoV, and SARS-CoV-2. These viruses are responsible for SARS, MERS, and CoV disease 2019 (COVID-19), respectively. A variety of diagnostic techniques, including chest X-rays, computer tomography (CT) scans, analysis of viral nucleic acids, proteins, or whole virions, and host antibody detection using serological assays have... 

    A foreign body response-on-a-chip platform

    , Article Advanced Healthcare Materials ; Volume 8, Issue 4 , 2019 ; 21922640 (ISSN) Sharifi, F ; Htwe, S. S ; Righi, M ; Liu, H ; Pietralunga, A ; Yesil Celiktas, O ; Maharjan, S ; Cha, B. H ; Shin, S. R ; Dokmeci, M. R ; Vrana, N. E ; Ghaemmaghami, A. M ; Khademhosseini, A ; Zhang, Y. S ; Sharif University of Technology
    Wiley-VCH Verlag  2019
    Understanding the foreign body response (FBR) and desiging strategies to modulate such a response represent a grand challenge for implant devices and biomaterials. Here, the development of a microfluidic platform is reported, i.e., the FBR-on-a-chip (FBROC) for modeling the cascade of events during immune cell response to implants. The platform models the native implant microenvironment where the implants are interfaced directly with surrounding tissues, as well as vasculature with circulating immune cells. The study demonstrates that the release of cytokines such as monocyte chemoattractant protein 1 (MCP-1) from the extracellular matrix (ECM)-like hydrogels in the bottom tissue chamber... 

    Early detection of immunization: A study based on an animal model using 1H nuclear magnetic resonance spectroscopy

    , Article Pakistan Journal of Biological Sciences ; Volume 14, Issue 3 , 2011 , Pages 195-203 ; 10288880 (ISSN) Zamani, Z ; Arjmand, M ; Tafazzoli, M ; Ghohzadeh, A ; Pourfallah, F ; Sadeghi, S ; Mirzazadeh, R ; Mirkham, F ; Tahen, S ; Iravam, A ; Bayat, P ; Vahabi, F ; Sharif University of Technology
    Vaccines require a period of at least three months for clinical trials, hence a method that can identify elicitation of immune response a few days after the first dose is a necessity. Evolutionary variable selections are modeling approaches for proper manipulation of available data which were used to set up an animal model for classification of time dependent 'HNMR metabolomic profiles and pattern recognition of fluctuations of metabolites in two groups of male rabbits. One group of rabbits was immunized with human red blood cells and the other used as control. Blood was obtained every 48 h from each rabbit for a period of six weeks and the serum monitored for antibodies and metabolites by... 

    Intralesional immunotherapy compared to cryotherapy in the treatment of warts

    , Article International Journal of Dermatology ; Volume 56, Issue 4 , 2017 , Pages 474-478 ; 00119059 (ISSN) Khozeimeh, F ; Jabbari Azad, F ; Mahboubi Oskouei, Y ; Jafari, M ; Tehranian, S ; Alizadehsani, R ; Layegh, P ; Sharif University of Technology
    Blackwell Publishing Ltd  2017
    Background: Warts are the most common clinical manifestation of the human papilloma-virus infection in the skin and mucous membranes. In spite of the various therapeutic modalities for nongenital skin warts, there is still no single method to be used as an approved treatment. In this study, we compared the efficacy of immunotherapy and cryotherapy on wart lesions. Methods: Sixty patients with verruca vulgaris and plantar warts were randomly divided into two groups. One group received intralesional injection of candida antigen repeated every 3 weeks until complete improvement of all warts or for a maximum of three sessions. The second group was treated by cryotherapy with liquid nitrogen for... 

    Electrospun polyethersolfone nanofibrous membrane as novel platform for protein immobilization in microfluidic systems

    , Article Journal of Biomedical Materials Research - Part B Applied Biomaterials ; Volume 106, Issue 3 , April , 2018 , Pages 1108-1120 ; 15524973 (ISSN) Mahmoudifard, M ; Vossoughi, M ; Soudi, S ; Soleimani, M ; Sharif University of Technology
    John Wiley and Sons Inc  2018
    In the present study, the feasibility of electrospun polyethersolfone (PES) nanofibrous membrane as the solid substrate for microfluidic based immunoassays to enhance the density of immobilized antibody on the surface of membrane was assessed. Conversely, the efficacy of antibody immobilization was compared by two different strategies as 1-Ethyl-3-(3-dimethylaminopropyl)-carbodiimide (EDC)/N-Hydroxysuccinimide (NHS) coupling chemistry and hydrophobic interaction. Compared to conventional immunoassays carried out in plates or gels, microfluidic based immunoassays grant a lot of advantages such as a consumption of little samples and reagents, shorter analysis time, and higher efficiency.... 

    Inactivation methods for whole influenza vaccine production

    , Article Reviews in Medical Virology ; Volume 29, Issue 6 , 2019 ; 10529276 (ISSN) Sabbaghi, A ; Miri, S. M ; Keshavarz, M ; Zargar, M ; Ghaemi, A ; Sharif University of Technology]
    John Wiley and Sons Ltd  2019
    Despite tremendous efforts toward vaccination, influenza remains an ongoing global threat. The induction of strain-specific neutralizing antibody responses is a common phenomenon during vaccination with the current inactivated influenza vaccines, so the protective effect of these vaccines is mostly strain-specific. There is an essential need for the development of next-generation vaccines, with a broad range of immunogenicity against antigenically drifted or shifted influenza viruses. Here, we evaluate the potential of whole inactivated vaccines, based on chemical and physical methods, as well as new approaches to generate cross-protective immune responses. We also consider the mechanisms by... 

    Gut-on-a-chip: Current progress and future opportunities

    , Article Biomaterials ; Volume 255 , 2020 Ashammakhi, N ; Nasiri, R ; Barros, N. R. D ; Tebon, P ; Thakor, J ; Goudie, M ; Shamloo, A ; Martin, M. G ; Khademhosseni, A ; Sharif University of Technology
    Elsevier Ltd  2020
    Organ-on-a-chip technology tries to mimic the complexity of native tissues in vitro. Important progress has recently been made in using this technology to study the gut with and without microbiota. These in vitro models can serve as an alternative to animal models for studying physiology, pathology, and pharmacology. While these models have greater physiological relevance than two-dimensional (2D) cell systems in vitro, endocrine and immunological functions in gut-on-a-chip models are still poorly represented. Furthermore, the construction of complex models, in which different cell types and structures interact, remains a challenge. Generally, gut-on-a-chip models have the potential to... 

    Mapping routine measles vaccination in low- and middle-income countries

    , Article Nature ; Volume 589, Issue 7842 , 2021 , Pages 415-419 ; 00280836 (ISSN) Sbarra, A. N ; Rolfe, S ; Nguyen, J.Q ; Earl, L ; Galles, N.C ; Marks, A ; Abbas, K.M ; Abbasi-Kangevari, M ; Abbastabar, H ; Abd-Allah, F ; Abdelalim, A ; Abdollahi, M ; Abegaz, K.H ; Abiy, H.A.A ; Abolhassani, H ; Abreu, L.G ; Abrigo, M.R.M ; Abushouk, A.I ; Accrombessi, M.M.K ; Adabi, M ; Adebayo, O.M ; Adekanmbi, V ; Adetokunboh, O.O ; Adham, D ; Afarideh, M ; Aghaali, M ; Ahmad, T ; Ahmadi, R ; Ahmadi, K ; Ahmed, M.B ; Alanezi, F.M ; Alanzi, T.M ; Alcalde-Rabanal, J.E ; Alemnew, B.T ; Ali, B.A ; Ali, M ; Alijanzadeh, M ; Alinia, C ; Alipoor, R ; Alipour, V ; Alizade, H ; Aljunid, S.M ; Almasi, A ; Almasi-Hashiani, A ; Al-Mekhlafi, H.M ; Altirkawi, K.A ; Amare, B ; Amini, S ; Amini-Rarani, M ; Amiri, F ; Amit, A.M.L ; Amugsi, D.A ; Ancuceanu, R ; Andrei, C.L ; Anjomshoa, M ; Ansari, F ; Ansari-Moghaddam, A ; Ansha, M.G ; Antonio, C.A.T ; Antriyandarti, E ; Anvari, D ; Arabloo, J ; Arab-Zozani, M ; Aremu, O ; Armoon, B ; Aryal, K.K ; Arzani, A ; Asadi-Aliabadi, M ; Asgari, S ; Atafar, Z ; Ausloos, M ; Awoke, N ; Quintanilla, B.P.A ; Ayanore, M.A ; Aynalem, Y.A ; Azadmehr, A ; Azari, S ; Babaee, E ; Badawi, A ; Badiye, A.D ; Bahrami, M.A ; Baig, A.A ; Bakhtiari, A ; Balakrishnan, S ; Banach, M ; Banik, P.C ; Barac, A ; Baradaran-Seyed, Z ; Baraki, A.G ; Basu, S ; Bayati, M ; Bayou, Y.T ; Bedi, N ; Behzadifar, M ; Bell, M.L ; Berbada, D.A ; Berhe, K ; Bhattarai, S ; Bhutta, Z.A ; Bijani, A ; Birhanu, M ; Bisanzio, D ; Biswas, A ; Bohlouli, S ; Bolla, S.R ; Borzouei, S ; Brady, O.J ; Bragazzi, N.L ; Briko, A.N ; Briko, N.I ; Nagaraja, S.B ; Butt, Z.A ; Cámera, L.A ; Campos-Nonato, I.R ; Car, J ; Cárdenas, R ; Carvalho, F ; Castaldelli-Maia, J.M ; Castro, F ; Chattu, V.K ; Chehrazi, M ; Chin, K.L ; Chu, D.-T ; Cook, A.J ; Cormier, N.M ; Cunningham, B ; Dahlawi, S.M.A ; Damiani, G ; Dandona, R ; Dandona, L ; Danovaro, M.C ; Dansereau, E ; Daoud, F ; Darwesh, A.M ; Darwish, A.H ; Das, J.K ; Weaver, N.D ; De Neve, J.-W ; Demeke, F.M ; Demis, A.B ; Denova-Gutiérrez, E ; Desalew, A ; Deshpande, A ; Desta, D.M ; Dharmaratne, S.D ; Dhungana, G.P ; Dianatinasab, M ; Diaz, D ; Dipeolu, I.O ; Djalalinia, S ; Do, H. T ; Dorostkar, F ; Doshmangir, L ; Doyle, K. E ; Dunachie, S. J ; Duraes, A. R ; Kalan, M. E ; Leylabadlo, H. E ; Edinur, H. A ; Effiong, A ; Eftekhari, A ; El Sayed, I ; El Sayed Zaki, M ; Elema, T. B ; Elhabashy, H. R ; El-Jaafary, S. I ; Elsharkawy, A ; Emamian, M. H ; Enany, S ; Eshrati, B ; Eskandari, K ; Eskandarieh, S ; Esmaeilnejad, S ; Esmaeilzadeh, F ; Esteghamati, A ; Etisso, A. E ; Farahmand, M ; Faraon, E. J. A ; Fareed, M ; Faridnia, R ; Farioli, A ; Farzadfar, F ; Fattahi, N ; Fazlzadeh, M ; Fereshtehnejad, S. M ; Fernandes, E ; Filip, I ; Fischer, F ; Foigt, N. A ; Folayan, M. O ; Foroutan, M ; Fukumoto, T ; Fullman, N ; Gad, M. M ; Geberemariyam, B. S ; Gebrehiwot, T. T ; Gebrehiwot, A. M ; Gebremariam, K. T ; Gebremedhin, K. B ; Gebremeskel, G. G ; Gebreslassie, A. A ; Gedefaw, G. A ; Gezae, K. E ; Ghadiri, K ; Ghaffari, R ; Ghaffarifar, F ; Ghajarzadeh, M ; Gheshlagh, R. G ; Ghashghaee, A ; Ghiasvand, H ; Gholamian, A ; Gilani, S. A ; Gill, P. S ; Girmay, A ; Gomes, N. G. M ; Gopalani, S. V ; Goulart, B. N. G ; Grada, A ; Guimarães, R. A ; Guo, Y ; Gupta, R ; Hafezi Nejad, N ; Haj-Mirzaian, A ; Haj-Mirzaian, A ; Handiso, D. W ; Hanif, A ; Haririan, H ; Hasaballah, A. I ; Hasan, M. M ; Hasanpoor, E ; Hasanzadeh, A ; Hassanipour, S ; Hassankhani, H ; Heidari Soureshjani, R ; Henry, N. J ; Herteliu, C ; Heydarpour, F ; Hollerich, G. I ; Rad, E. H ; Hoogar, P ; Hossain, N ; Hosseini, M ; Hosseinzadeh, M ; Househ, M ; Hu, G ; Huda, T. M ; Humayun, A ; Ibitoye, S. E ; Ikilezi, G ; Ilesanmi, O. S ; Ilic, I. M ; Ilic, M. D ; Imani Nasab, M. H ; Inbaraj, L.R ; Iqbal, U ; Irvani, S. S. N ; Islam, S. M. S ; Islam, M. M ; Iwu, C. J ; Iwu, C. C. D ; Jadidi Niaragh, F ; Jafarinia, M ; Jahanmehr, N ; Jakovljevic, M ; Jalali, A ; Jalilian, F ; Javidnia, J ; Jenabi, E ; Jha, V ; Ji, J. S ; John, O ; Johnson, K. B ; Joukar, F ; Jozwiak, J. J ; Kabir, Z ; Kabir, A ; Kalani, H ; Kalankesh, L. R ; Kalhor, R ; Kamal, Z ; Kanchan, T ; Kapoor, N ; Karami, M ; Matin, B. K ; Karch, A ; Karimi, S. E ; Kayode, G. A ; Karyani, A. K ; Keiyoro, P. N ; Khader, Y. S ; Khafaie, M. A ; Khammarnia, M ; Khan, M. S ; Khan, E. A ; Khan, J ; Khan, M. N ; Khatab, K ; Khater, M. M ; Khatib, M. N ; Khayamzadeh, M ; Khazaei, M ; Khazaei, S ; Khosravi, A ; Khubchandani, J ; Kianipour, N ; Kim, Y. J ; Kimokoti, R. W ; Kinyoki, D. K ; Kisa, A ; Kisa, S ; Kolola, T ; Komaki, H ; Kosen, S ; Koul, P.A ; Koyanagi, A ; Kraemer, M. U. G ; Krishan, K ; Kuate Defo, B ; Kumar, M ; Kumar, P ; Kumar, G. A ; Kusuma, D ; La Vecchia, C ; Lacey, B ; Lad, S. D ; Lal, D. K ; Lam, F ; Lami, F. H ; Lansingh, V. C ; Larson, H. J ; Lasrado, S ; Lee, S. W. H ; Lee, P. H ; LeGrand, K. E ; Lenjebo, T. L ; Li, S ; Liang, X ; Liu, P.Y ; Lopukhov, P. D ; Machado, D. B ; Mahasha, P. W ; Mahdavi, M. M ; Maheri, M ; Mahotra, N. B ; Maled, V ; Maleki, S ; Malik, M. A ; Malta, D. C ; Mansour Ghanaei, F ; Mansouri, B ; Mansourian, M ; Mansournia, M. A ; Martins Melo, F. R ; Masaka, A ; Mayala, B. K ; Mehndiratta, M. M ; Mehri, F ; Mehta, K. M ; Memiah, P. T. N ; Mendoza, W ; Menezes, R. G ; Mengesha, M. B ; Mengesha, E. W ; Mestrovic, T ; Mihretie, K. M ; Miller Petrie, M. K ; Mills, E. J ; Milne, G. J ; Mirabi, P ; Mirrakhimov, E. M ; Mirzaei, R ; Mirzaei, M ; Mirzaei, H. R ; Mirzaei, H ; Mirzaei Alavijeh, M ; Moazen, B ; Moghadaszadeh, M ; Mohamadi, E ; Mohammad, D. K ; Mohammad, Y ; Mohammad, K. A ; Mohammad Gholi Mezerji, N ; Mohammadbeigi, A ; Mohammadian Hafshejani, A ; Mohammadpourhodki, R ; Mohammed, S ; Mohammed, A. S ; Mohammed, H ; Mohebi, F ; Mokdad, A. H ; Monasta, L ; Moosavi, M. A ; Moosazadeh, M ; Moradi, G ; Moradi, M ; Moradi Joo, M ; Moradi Lakeh, M ; Moradzadeh, R ; Moraga, P ; Mosapour, A ; Sharif University of Technology
    Nature Research  2021
    The safe, highly effective measles vaccine has been recommended globally since 1974, yet in 2017 there were more than 17 million cases of measles and 83,400 deaths in children under 5 years old, and more than 99% of both occurred in low- and middle-income countries (LMICs)1–4. Globally comparable, annual, local estimates of routine first-dose measles-containing vaccine (MCV1) coverage are critical for understanding geographically precise immunity patterns, progress towards the targets of the Global Vaccine Action Plan (GVAP), and high-risk areas amid disruptions to vaccination programmes caused by coronavirus disease 2019 (COVID-19)5–8. Here we generated annual estimates of routine childhood... 

    Antitumor effect of therapeutic HPV DNA vaccines with chitosan-based nanodelivery systems

    , Article Journal of Biomedical Science ; Vol. 21, issue. 1 , July , 2014 ; ISSN: 10217770 Tahamtan, A ; Ghaemi, A ; Gorji, A ; Kalhor, H. R ; Sajadian, A ; Tabarraei, A ; Moradi, A ; Atyabi, F ; Kelishadi, M ; Sharif University of Technology
    Cervical cancer is the second-most-common cause of malignancies in women worldwide, and the oncogenic activity of the human papilloma virus types (HPV) E7 protein has a crucial role in anogenital tumors. In this study, we have designed a therapeutic vaccine based on chitosan nanodelivery systems to deliver HPV-16 E7 DNA vaccine, considered as a tumor specific antigen for immunotherapy of HPV-associated cervical cancer. We have developed a Nano-chitosan (NCS) as a carrier system for intramuscular administration using a recombinant DNA vaccine expressing HPV-16 E7 (NCS-DNA E7 vaccine). NCS were characterized in vitro for their gene transfection ability. Results: The transfection of CS-pEGFP... 

    Prevalence of influenza a infection in the middle-east: a systematic review and meta-analysis

    , Article Clinical Respiratory Journal ; Volume 12, Issue 5 , 2018 , Pages 1787-1801 ; 17526981 (ISSN) Moghoofei, M ; Monavari, H. R ; Mostafaei, S ; Hadifar, S ; Ghasemi, A ; Babaei, F ; Kavosi, H ; Tavakoli, A ; Javanmard, D ; Esghaei, M ; Khodabandehlou, N ; Sharif University of Technology
    Blackwell Publishing Ltd  2018
    Objective: This systematic review and meta-analysis was performed to determine the prevalence rate of influenza virus from different parts of Middle East region, and present an overall relative frequency (RF) for this region. Methods: The authors performed a systematic literature review from several reliable databases such as PubMed, ISI Web of Science and Scopus during 2000–2016. Furthermore, the keywords of this research were ‘Influenza’, ‘Subtype’, ‘Seroprevalence’, ‘Incidence’, ‘Seroepidemiology’, ‘H1N1’, ‘H3N2’, ‘H5N1’, ‘H9N2’, ‘Middle-East’ and ‘Meta-analysis’. The reported data were selected according to inclusion and exclusion criteria. Results: The authors selected 71 studies out of... 

    Development of a novel nano-sized anti-VEGFA nanobody with enhanced physicochemical and pharmacokinetic properties

    , Article Artificial Cells, Nanomedicine and Biotechnology ; Volume 46, Issue 7 , 2018 , Pages 1402-1414 ; 21691401 (ISSN) Khodabakhsh, F ; Norouzian, D ; Vaziri, B ; Ahangari Cohan, R ; Sardari, S ; Mahboudi, F ; Behdani, M ; Mansouri, K ; Mehdizadeh, A ; Sharif University of Technology
    Since physiological and pathological processes occur at nano-environments, nanotechnology has considered as an efficient tool for designing of next generation specific biomolecules with enhanced pharmacodynamic and pharmacodynamic properties. In the current investigation, by control of the size and hydrodynamic volume at the nanoscale, for the first time, physicochemical and pharmacokinetic properties of an anti-VEGFA nanobody was remarkably improved by attachment of a Proline-Alanine-Serine (PAS) rich sequence. The results elucidated unexpected impressive effects of PAS sequence on physicochemical properties especially on size, hydrodynamics radius, and even solubility of nanobody. CD...