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infertility
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A novel variant in TLE6 is associated with embryonic developmental arrest (EDA) in familial female infertility
, Article Scientific Reports ; Volume 12, Issue 1 , 2022 ; 20452322 (ISSN) ; Mohebi, M ; Berjis, K ; Ghahremani, A ; Modarresi, M. H ; Ghafouri Fard, S ; Sharif University of Technology
Nature Research
2022
Abstract
This study aims to identify genetic causes of familial female infertility characterized by embryonic developmental arrest (EDA) and repeated implantation failure (RIF) with oocyte donation IVF cycle. We used Whole-exome sequencing and Sanger validation to find causative genes in an Iranian consanguineous family that had 3 infertile daughters, 4 fertile daughters, and 2 fertile sons. All patients in this consanguineous family exhibited typical manifestations of unexplained RIF and EDA. Genetic analysis identified a homozygous missense variant (c.G1054C:p.G352R) in exon 13 of the TLE6 gene that cosegregated with the EDA phenotype in an autosomal recessive pattern. Other members of the family,...
Design and Fabrication of Microfluidic Chip for Selection of Functional Sperm
, M.Sc. Thesis Sharif University of Technology ; Saadatmand, Maryam (Supervisor) ; Abdekhodaie, Mohammad Jafar (Supervisor) ; Kazemnejad, Somaieh (Co-Supervisor)
Abstract
Infertility is a growing global issue with significant psychological, economic, and social consequences, which among them, male factor infertility problems make up a considerable portion. The conventional methods of sperm separation inclusive of density-gradient centrifugation and swim-up with numerous problems contain DNA fragmentation and low yield, and both of the methods are not effective for samples with low sperm counts. Modern microfluidic systems are trying to improve this problem by increasing the quality of the isolated sperms. However, most of these systems rely solely on sperm motility as a separation mechanism, which is not a sufficient condition for the separated sperm to be...
Tracking of Human Sperm Cell using a Dynamic Bayesian Network Based Framework
, Ph.D. Dissertation Sharif University of Technology ; Vosoughi Vahdat, Bijan (Supervisor) ; Salman Yazdi, Reza (Co-Supervisor)
Abstract
Infertility is an important problem to deal in medicine. In every four couples, on average, one couple is affected by infertility in developing countries. In the majority of cases, the infertility of men has a relationship with spermatozoa and semen, and can be measured by semen and spermatozoa analysis for more advanced diagnosis and treatments. Analysis of the movement patterns of spermatozoa were performed by expert screeners earlier, but nowadays, many of these analyzes are performed using computer-based systems called computer assisted sperm analysis (CASA). The benefits of using CASA instead of expert screeners are achieving system-independency and numerical results at the end of the...
The influence of the female reproductive tract and sperm features on the design of microfluidic sperm-sorting devices
, Article Journal of Assisted Reproduction and Genetics ; Volume 39, Issue 1 , 2022 , Pages 19-36 ; 10580468 (ISSN) ; Hosseini, M ; Saadatmand, M ; Abbaspourrad, A ; Sharif University of Technology
Springer
2022
Abstract
Although medical advancements have successfully helped a lot of couples with their infertility by assisted reproductive technologies (ART), sperm selection, a crucial stage in ART, has remained challenging. Therefore, we aimed to investigate novel sperm separation methods, specifically microfluidic systems, as they do sperm selection based on sperm and/or the female reproductive tract (FRT) features without inflicting any damage to the selected sperm during the process. In this review, after an exhaustive studying of FRT features, which can implement by microfluidics devices, the focus was centered on sperm selection and investigation devices. During this study, we tried not to only point to...
Metabolomics fingerprinting of the human seminal plasma of asthenozoospermic patients
, Article Molecular Reproduction and Development ; Vol. 81, Issue. 1 , 2014 , pp. 84-86 ; ISSN: 1098-2795 ; Moazeni-Pourasil, R. S ; Jafarzadeh, N ; Savadi-Shiraz, E ; Sharif University of Technology
Abstract
It is estimated that 20% of couples are infertile, and half of these infertility cases are linked to men. One of conditions that can affect male fertility is asthenozoospermia. We applied Raman spectroscopy to the analysis of the metabolome of the human seminal plasma, and used chemometrics on the patterns of Raman spectra obtained. Significant changes were observed in the metabolome of the human seminal plasma of asthenozoospermic patients
Metabolic profiling of seminal plasma from teratozoospermia patients
, Article Journal of Pharmaceutical and Biomedical Analysis ; Volume 178 , January , 2020 ; Chashmniam, S ; Nobakht, F ; Amini, M ; Javidi, A ; Minai Tehrani, A ; Arjmand, B ; Gilany, K ; Sharif University of Technology
Elsevier B. V
2020
Abstract
Teratozoospermia is one of conditions that can cause male infertility. The mechanism of teratozoospermia remains unclear. The knowledge of the metabolites in human seminal plasma (HSP) is meaningful for the pathological study of teratozoospermia. Analysis of changed metabolites in HSP can help understand the cellular mechanism, find the novel biomarkers and subsequently design a diagnosis test. In this study, the analysis of samples performed by proton nuclear magnetic resonance spectroscopy (1H NMR spectroscopy) to identify the various metabolites, with the aim of finding metabolic profiles and biomarkers related to male infertility. Eighteen de-regulated metabolites were identified in...
Automatic Analysis and Tracking of Motile Cells in Video Microscopy
, M.Sc. Thesis Sharif University of Technology ; Vosughi Vahdat, Bijan (Supervisor) ; Rabiei, Hamid Reza (Supervisor) ; Salman Yazdi, Reza (Co-Advisor)
Abstract
Analysis of semen and quality assessments of sperm cells is of great importance in scrutiny of male fertility. Several methods have been introduced for analyzing and identification of the sperm motility and morphology in a semen sample. Identifying and tracking of rapid and variant movements of multiple sperms, in a short duration of time, is somewhat difficult and complex for human, even for an expert. Then applying semi-automated or automated (un-supervised) methods, based on image analysis and computing, spread fast and computer aided semen analyzer systems, became wildly used in clinical and research laboratories.
In this paper we propose an efficient multiple tracking methods to...
In this paper we propose an efficient multiple tracking methods to...
Microfluidic-based droplets for advanced regenerative medicine: current challenges and future trends
, Article Biosensors ; Volume 12, Issue 1 , 2022 ; 20796374 (ISSN) ; Heirani Tabasi, A ; Ghorbani, S ; Eyni, H ; Razavi Bazaz, S ; Khayati, M ; Gheidari, F ; Moradpour, K ; Kehtari, M ; Ahmadi Tafti, S.M ; Ahmadi Tafti, S. H ; Warkiani, M. E ; Sharif University of Technology
MDPI
2022
Abstract
Microfluidics is a promising approach for the facile and large-scale fabrication of monodispersed droplets for various applications in biomedicine. This technology has demonstrated great potential to address the limitations of regenerative medicine. Microfluidics provides safe, accurate, reliable, and cost-effective methods for encapsulating different stem cells, gametes, biomaterials, biomolecules, reagents, genes, and nanoparticles inside picoliter-sized droplets or droplet-derived microgels for different applications. Moreover, microenvironments made using such droplets can mimic niches of stem cells for cell therapy purposes, simulate native extracellular matrix (ECM) for tissue...