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inorganic-mercury--hg2--
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Development of a novel method for determination of mercury based on its inhibitory effect on horseradish peroxidase activity followed by monitoring the surface plasmon resonance peak of gold nanoparticles
, Article Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy ; Volume 153 , 2016 , Pages 709-713 ; 13861425 (ISSN) ; Haji Shabani, A. M ; Dadfarnia, S ; Rohani Moghadam, M. R ; Hormozi Nezhad, M. R ; Sharif University of Technology
Elsevier
Abstract
A highly sensitive and simple indirect spectrophotometric method has been developed for the determination of trace amounts of inorganic mercury (Hg2 +) in aqueous media. The method is based on the inhibitory effect of Hg2 + on the activity of horseradish peroxidase (HRP) in the oxidation of ascorbic acid by hydrogen peroxide followed by the reduction of Au3 + to Au-NPs by unreacted ascorbic acid and the measurement of the absorbance of localized surface plasmon resonance (LSPR) peak of gold nanoparticles (at 530 nm) which is directly proportional to the concentration of Hg2 +. Under the optimum conditions, the calibration curve was linear in the concentration range of 1-220 ng mL- 1. Limits...