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    Surface Modification of Bacterial Cellulose-Reinforced Keratin Nanofibers using Pluronic/Gum Tragacanth Hydrogel Nanoparticles Produced by Concurrent gel Electrospray/Polymer Electrospinning Method

    , M.Sc. Thesis Sharif University of Technology Azarniya, Amir (Author) ; Simchi, Abdolreza (Supervisor) ; Tamjid, Elnaz (Supervisor)
    In this work, wool keratin/polyethylene oxide (PEO) nanofibrous scaffolds were fabricated by electrospinning method. Bacterial cellulose nanofibrils (BCNFs) were embedded in the electrospun keratin/PEO nanofibers. Incorporation of BCNFs into the nanofibers enhances their hydrophilicity, mechanical properties and cell viability, adhesion and proliferation. Water contact angle of the nanofibers decreased from 126˚ to 83˚by addition of 1 wt % BCNFs. A thermogelling hydrogel based on carboxylated pluronic (Pl-COOH) and gum tragacanth (GT) was fabricated and polymer conjugation was confirmed by FTIR and H-NMR spectroscopy. Morphological and viscoelastic properties of GT-grafted Pl-COOH hydrogels... 

    Modification of bacterial cellulose/keratin nanofibrous mats by a tragacanth gum-conjugated hydrogel for wound healing

    , Article International Journal of Biological Macromolecules ; Volume 134 , 2019 , Pages 280-289 ; 01418130 (ISSN) Azarniya, A ; Tamjid, E ; Eslahi, N ; Simchi, A ; Sharif University of Technology
    Elsevier B.V  2019
    To enhance physicomechanical properties and bioactivity of fibrous membranes for wound dressing and tissue engineering applications, novel composite scaffolds consisting of fibrous mats and thermosensitive hydrogel particles were prepared by concurrent electrospinning and electrospraying technique. The composite scaffolds were composed of keratin/bacterial cellulose fibers (150 ± 43 nm) which are hybridized with hydrogel particles (500 nm to 2 μm) based on nonionic triblock copolymers conjugated with Tragacanth gum (TG). FTIR and H-NMR studies indicated ester reactions between carboxylated copolymers and TG through carbodiimide crosslinker chemistry. The hydrogel particles were uniformly... 

    Dual-sensitive hydrogel nanoparticles based on conjugated thermoresponsive copolymers and protein filaments for triggerable drug delivery

    , Article ACS Applied Materials and Interfaces ; Volume 10, Issue 23 , 17 May , 2018 , Pages 19336-19346 ; 19448244 (ISSN) Ghaffari, R ; Eslahi, N ; Tamjid, E ; Simchi, A ; Sharif University of Technology
    American Chemical Society  2018
    In this study, novel hydrogel nanoparticles with dual triggerable release properties based on fibrous structural proteins (keratin) and thermoresponsive copolymers (Pluronic) are introduced. Nanoparticles were used for curcumin delivery as effective and safe anticancer agents, the hydrophobicity of which has limited their clinical applications. A drug was loaded into hydrogel nanoparticles by a single-step nanoprecipitation method. The drug-loaded nanoparticles had an average diameter of 165 and 66 nm at 25 and 37 °C, respectively. It was shown that the drug loading efficiency could be enhanced through crosslinking of the disulfide bonds in keratin. Crosslinking provided a targeted release... 

    In vitro co-culture of human skin keratinocytes and fibroblasts on a biocompatible and biodegradable scaffold

    , Article Iranian Biomedical Journal ; Volume 13, Issue 3 , 2009 , Pages 169-177 ; 1028852X (ISSN) Shariati, S. R. P ; Shokrgozar, M. A ; Vossoughi, M ; Eslamifar, A ; Sharif University of Technology
    Background: Extensive full-thickness burns require replacement of both epidermis and dermis. In designing skin replacements, the goal has been to re-create this model and make a product which has both essential components. Methods: In the present study, we developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface of modified collagen-chitosan scaffold that contains fibroblasts. The culture methods for propagation of keratinocytes and fibroblasts isolated from human neonatal foreskin were developed. The growth and proliferation of normal human keratinocytes were evaluated in serum-free (keratinocyte growth medium) and our modified... 

    Fabrication and characterization of core-shell electrospun fibrous mats containing medicinal herbs for wound healing and skin tissue engineering

    , Article Marine Drugs ; Volume 17, Issue 1 , 2019 ; 16603397 (ISSN) Zahedi, E ; Esmaeili, A ; Eslahi, N ; Shokrgozar, M. A ; Simchi, A ; Sharif University of Technology
    MDPI AG  2019
    Nanofibrous structures mimicking the native extracellular matrix have attracted considerable attention for biomedical applications. The present study aims to design and produce drug-eluting core-shell fibrous scaffolds for wound healing and skin tissue engineering. Aloe vera extracts were encapsulated inside polymer fibers containing chitosan, polycaprolactone, and keratin using the co-axial electrospinning technique. Electron microscopic studies show that continuous and uniform fibers with an average diameter of 209 ± 47 nm were successfully fabricated. The fibers have a core-shell structure with a shell thickness of about 90 nm, as confirmed by transmission electron microscopy. By... 

    Study of epithelial differentiation and protein expression of keratinocyte-mesenchyme stem cell co-cultivation on electrospun nylon/B. vulgaris extract composite scaffold

    , Article Materials Science and Engineering C ; Volume 75 , 2017 , Pages 653-662 ; 09284931 (ISSN) Hosseinzadeh, S ; Soleimani, M ; Vossoughi, M ; Ranjbarvan, P ; Hamedi, S ; Zamanlui, S ; Mahmoudifard, M ; Sharif University of Technology
    Employing of the composite electrospun scaffold containing herbal extract in conjugation with co-culturing of cells can open up new window to the design of efficient biomaterials for skin tissue regeneration. Here, we introduce the synergistic effect of composite electrospun nanofibrous scaffold of nylon66 loaded with Beta vulgaris (B. vulgaris) (extract of beet roots, a plants whose widely used in Iranian folk medicine as wound healing medicine) and co-culture of mesenchymal stem-cells (MSCs)-human keratinocyte (H-keratino) differentiation towards epithelial lineage. In vitro biocompatibility was examined through MTT assay and epithelial differentiation checked by real-time PCR and... 

    Natural compounds for skin tissue engineering by electrospinning of nylon-Beta vulgaris

    , Article ASAIO Journal ; Volume 64, Issue 2 , 2018 , Pages 261-269 ; 10582916 (ISSN) Ranjbarvan, P ; Mahmoudifard, M ; Kehtari, M ; Babaie, A ; Hamedi, S ; Mirzaei, S ; Soleimani, M ; Hosseinzadeh, S ; Sharif University of Technology
    Lippincott Williams and Wilkins  2018
    Natural compounds containing polysaccharide ingredients have been employed as candidates for treatment of skin tissue. Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering. Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle. Scanning electron microscope examinations have approved the uniform and homogeneous...