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    Transplantation of autologous epidermal melanocyte–keratinocyte cells suspension for cell therapy of vitiligo: A clinical evaluation and biometric assessment

    , Article Journal of Cosmetic Dermatology ; Volume 21, Issue 12 , 2022 , Pages 7147-7152 ; 14732130 (ISSN) Nilforoushzadeh, M. A ; Farshi, S ; Nouri, M ; Alavi, S ; Bayat Tork, B ; Jaffary, F ; Zare, S ; Sharif University of Technology
    John Wiley and Sons Inc  2022
    Abstract
    Introduction: Among several surgical treatments, the use of transplantation of epidermal cultured melanocytes or melanocytes–keratinocytes cell suspension has gained many researchers and dermatologists' attention as a new technique for the treatment of vitiligo. The present study aimed to transplant autologous epidermal melanocytes–keratinocytes cell suspension for the treatment of vitiligo. Methods: In this study, 15 volunteer patients aged between 18 and 45 years old were studied. The autologous melanocytes–keratinocytes cell suspension was then transplanted to the region after dermabrasion. The included patients were evaluated by VisioFace, MPA9, and Skin Scanner-DUB once before and 1, 2,... 

    In vitro co-culture of human skin keratinocytes and fibroblasts on a biocompatible and biodegradable scaffold

    , Article Iranian Biomedical Journal ; Volume 13, Issue 3 , 2009 , Pages 169-177 ; 1028852X (ISSN) Shariati, S. R. P ; Shokrgozar, M. A ; Vossoughi, M ; Eslamifar, A ; Sharif University of Technology
    2009
    Abstract
    Background: Extensive full-thickness burns require replacement of both epidermis and dermis. In designing skin replacements, the goal has been to re-create this model and make a product which has both essential components. Methods: In the present study, we developed procedures for establishing confluent, stratified layers of cultured human keratinocytes on the surface of modified collagen-chitosan scaffold that contains fibroblasts. The culture methods for propagation of keratinocytes and fibroblasts isolated from human neonatal foreskin were developed. The growth and proliferation of normal human keratinocytes were evaluated in serum-free (keratinocyte growth medium) and our modified... 

    Producing functional recombinant human keratinocyte growth factor in Pichia pastoris and investigating its protective role against irradiation

    , Article Enzyme and Microbial Technology ; Volume 111 , April , 2018 , Pages 12-20 ; 01410229 (ISSN) Bahadori, Z ; Kalhor, H. R ; Mowla, S. J ; Sharif University of Technology
    Elsevier Inc  2018
    Abstract
    Keratinocyte Growth Factor (KGF) is a paracrine-acting, epithelial mitogen that plays a prominent role in the regeneration of damaged epithelial tissues. In spite of different attempts to produce recombinant human KGF in many organisms, including bacteria, mammalian cells, plant cells and insect cells; production of recombinant form suffers from lower yields and recovery relative to other recombinant proteins of similar size and properties. Due to many advantages of Pichia pastoris expression systems for producing industrial enzymes and pharmaceutical proteins, in this study P. pastoris was chosen as a host for KGF expression. For preparing human KGF coding sequence, MCF-7 cell line was... 

    Study of epithelial differentiation and protein expression of keratinocyte-mesenchyme stem cell co-cultivation on electrospun nylon/B. vulgaris extract composite scaffold

    , Article Materials Science and Engineering C ; Volume 75 , 2017 , Pages 653-662 ; 09284931 (ISSN) Hosseinzadeh, S ; Soleimani, M ; Vossoughi, M ; Ranjbarvan, P ; Hamedi, S ; Zamanlui, S ; Mahmoudifard, M ; Sharif University of Technology
    Abstract
    Employing of the composite electrospun scaffold containing herbal extract in conjugation with co-culturing of cells can open up new window to the design of efficient biomaterials for skin tissue regeneration. Here, we introduce the synergistic effect of composite electrospun nanofibrous scaffold of nylon66 loaded with Beta vulgaris (B. vulgaris) (extract of beet roots, a plants whose widely used in Iranian folk medicine as wound healing medicine) and co-culture of mesenchymal stem-cells (MSCs)-human keratinocyte (H-keratino) differentiation towards epithelial lineage. In vitro biocompatibility was examined through MTT assay and epithelial differentiation checked by real-time PCR and... 

    Defining microRNA signatures of hair follicular stem and progenitor cells in healthy and androgenic alopecia patients

    , Article Journal of Dermatological Science ; Volume 101, Issue 1 , 2021 , Pages 49-57 ; 09231811 (ISSN) Mohammadi, P ; Nilforoushzadeh, M. A ; Youssef, K. K ; Sharifi Zarchi, A ; Moradi, S ; Khosravani, P ; Aghdami, R ; Taheri, P ; Hosseini Salekdeh, G ; Baharvand, H ; Aghdami, N ; Sharif University of Technology
    Elsevier Ireland Ltd  2021
    Abstract
    Background: The exact pathogenic mechanism causes hair miniaturization during androgenic alopecia (AGA) has not been delineated. Recent evidence has shown a role for non-coding regulatory RNAs, such as microRNAs (miRNAs), in skin and hair disease. There is no reported information about the role of miRNAs in hair epithelial cells of AGA. Objectives: To investigate the roles of miRNAs affecting AGA in normal and patient's epithelial hair cells. Methods: Normal follicular stem and progenitor cells, as well as follicular patient's stem cells, were sorted from hair follicles, and a miRNA q-PCR profiling to compare the expression of 748 miRNA (miRs) in sorted cells were performed. Further, we... 

    Natural compounds for skin tissue engineering by electrospinning of nylon-Beta vulgaris

    , Article ASAIO Journal ; Volume 64, Issue 2 , 2018 , Pages 261-269 ; 10582916 (ISSN) Ranjbarvan, P ; Mahmoudifard, M ; Kehtari, M ; Babaie, A ; Hamedi, S ; Mirzaei, S ; Soleimani, M ; Hosseinzadeh, S ; Sharif University of Technology
    Lippincott Williams and Wilkins  2018
    Abstract
    Natural compounds containing polysaccharide ingredients have been employed as candidates for treatment of skin tissue. Herein, for the first time, electrospinning setup was proposed to fabricate an efficient composite nanofibrous structure of Beta vulgaris (obtained from Beet [Chenopodiaceae or Amaranthaceae]) belonged to polysaccharides and an elastic polymer named nylon 66 for skin tissue engineering. Both prepared scaffolds including noncomposite and composite types were studied by Scanning electron microscope (SEM), Fourier transform infrared (FTIR) spectroscopy, mechanical assay, and contact angle. Scanning electron microscope examinations have approved the uniform and homogeneous...