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    A graphene-based THz ring resonator for label-free sensing

    , Article IEEE Sensors Journal ; Volume 16, Issue 11 , 2016 , Pages 4338-4344 ; 1530437X (ISSN) Zangeneh Nejad, F ; Safian, R ; Sharif University of Technology
    Institute of Electrical and Electronics Engineers Inc 
    Abstract
    In this paper, we report on a novel resonant THz sensor for label-free analysis. The structure consists of a silicon nitride dielectric ring resonator vertically coupled to a thin layer of graphene strip ring resonator on top. The cladding is assumed to be porous alumina on the top of the graphene strip, which enhances the interaction of the surface plasmon wave and the target molecules. Finite difference time domain analysis is used to systematically design the structure and to investigate the performance of the sensor. Our simulations show that the proposed structure has larger refractive index sensitivity and lower intrinsic quality factor with respect to the similar optical structure. It... 

    A smart-phone based ratiometric nanoprobe for label-free detection of methyl parathion

    , Article Sensors and Actuators, B: Chemical ; Volume 322 , 2020 Fahimi Kashani, N ; Hormozi Nezhad, M. R ; Sharif University of Technology
    Elsevier B.V  2020
    Abstract
    The widespread use of pesticides in pest management has boosted the demands for developing highly sensitive probes for on-site monitoring. Herein we presented a sensitive enzyme-free ratiometric probe for determination of methyl parathion (MP), as an organophosphate pesticide using TGA-capped CdTe QDs and carbon dots (CDs). Unlike previous methods in which hydrolysis product of MP is instrumental in the response of the sensors, here, self-assembly of cetyltrimethylammonium bromide (CTAB) on the surface of non-modified yellow-emissive CdTe QDs facilitates the quenching of CTAB-QDs upon addition of MP while the fluorescence intensity of CDs remains constant. Using a smartphone, the ratiometric... 

    The ITO-capped WO3 nanowires biosensor based on field-effect transistor in label-free protein sensing

    , Article Applied Physics A: Materials Science and Processing ; Volume 123, Issue 5 , 2017 ; 09478396 (ISSN) Shariati, M ; Sharif University of Technology
    Springer Verlag  2017
    Abstract
    The fabrication of ITO-capped WO3 nanowires associated with their bio-sensing properties in field-effect transistor diagnostics basis as a biosensor has been reported. The bio-sensing property for manipulated nanowires elucidated that the grown nanostructures were very sensitive to protein. The ITO-capped WO3 nanowires biosensor showed an intensive bio-sensing activity against reliable protein. Polylysine strongly charged bio-molecule was applied as model system to demonstrate the implementation of materialized biosensor. The employed sensing mechanism was ‘label-free’ and depended on bio-molecule’s intrinsic charge. For nanowires synthesis, the vapor–liquid–solid mechanism was used.... 

    Label-free and simple detection of endotoxins using a sensitive LSPR biosensor based on silver nanocolumns

    , Article Analytical Biochemistry ; Volume 548 , 2018 , Pages 96-101 ; 00032697 (ISSN) Zandieh, M ; Hosseini, N ; Vossoughi, M ; Khatami, M ; Abbasian, S ; Moshaii, A ; Sharif University of Technology
    Academic Press Inc  2018
    Abstract
    This paper describes the construction of a silver-based LSPR biosensor for endotoxin detection. We used GLAD method to procure reproducible silver nanocolumns. In this work, the silver nanostructures were considerably stabilized by a SAM of MPA, and the limit of detection of biosensor was measured to be 340 pg/ml for endotoxin E. coli. Considering endotoxin B. abortus as the second type of endotoxin contamination in our target samples (HBs-ag produced in Institute Pasteur, Iran), we investigated selectivity of the biosensor in various experiments. We showed that this biosensor can selectively detect both types of endotoxins compared to other biological species. Overall, this study proposes... 

    Exploitation of n-gene of sars-cov-2 to develop a new rapid assay by ASOs@AuNPs

    , Article Analytical Chemistry ; Volume 94, Issue 39 , 2022 , Pages 13616-13622 ; 00032700 (ISSN) Borghei, Y. S ; Samadikhah, H. R ; Hosseinkhani, S ; Sharif University of Technology
    American Chemical Society  2022
    Abstract
    A naked-eye (equipment-free), label-free (cost-effective), and RNA extraction-free (to speed up) method for SARS-CoV-2 (as a case study of RNA viruses) detection is developed. Here, the DNA is being used as a template for in situ formation of anisotropic gold nanoparticles (AuNPs) without any chemical modification or DNA labeling. In this study, synthesized AuNPs for the direct detection of N-gene (nucleocapsid phosphoprotein) of SARS-CoV-2 are exploited. To this aim, antisense oligonucleotides (ASOs) with an extra poly guanine tail (G12) were designed. Thus, in the presence of its viral target RNA gene and ASOs@AuNPs-RNA hybridization, there was a red shift in its localized surface plasmon... 

    Label-free electrochemical microfluidic biosensors: futuristic point-of-care analytical devices for monitoring diseases

    , Article Microchimica Acta ; Volume 189, Issue 7 , 2022 ; 00263672 (ISSN) Ebrahimi, G ; Samadi Pakchin, P ; Shamloo, A ; Mota, A ; de la Guardia, M ; Omidian, H ; Omidi, Y ; Sharif University of Technology
    Springer  2022
    Abstract
    The integration of microfluidics with electrochemical analysis has resulted in the development of single miniaturized detection systems, which allows the precise control of sample volume with multianalyte detection capability in a cost- and time-effective manner. Microfluidic electrochemical sensing devices (MESDs) can potentially serve as precise sensing and monitoring systems for the detection of molecular markers in various detrimental diseases. MESDs offer several advantages, including (i) automated sample preparation and detection, (ii) low sample and reagent requirement, (iii) detection of multianalyte in a single run, (iv) multiplex analysis in a single integrated device, and (v)... 

    Mining the potential of label-free biosensors for in vitro antipsychotic drug screening

    , Article Biosensors ; Volume 8, Issue 1 , 2018 ; 20796374 (ISSN) Kilic, T ; Soler, M ; Fahimi Kashani, N ; Altug, H ; Carrara, S ; Sharif University of Technology
    MDPI AG  2018
    Abstract
    The pharmaceutical industry is facing enormous challenges due to high drug attribution rates. For the past decades, novel methods have been developed for safety and efficacy testing, as well as for improving early development stages. In vitro screening methods for drug-receptor binding are considered to be good alternatives for decreasing costs in the identification of drug candidates. However, these methods require lengthy and troublesome labeling steps. Biosensors hold great promise due to the fact that label-free detection schemes can be designed in an easy and low-cost manner. In this paper, for the first time in the literature, we aimed to compare the potential of label-free optical and... 

    Toward visual chiral recognition of amino acids using a wide-range color tonality ratiometric nanoprobe

    , Article Analytica Chimica Acta ; Volume 1231 , 2022 ; 00032670 (ISSN) Jafar Nezhad Ivrigh, Z ; Fahimi Kashani, N ; Morad, R ; Jamshidi, Z ; Hormozi Nezhad, M. R ; Sharif University of Technology
    Elsevier B.V  2022
    Abstract
    Chiral recognition has long been a challenging issue to deal with in biological systems, drug design and food authentication. Implementing nanoparticle-based probes with intrinsic or induced chirality in this field has addressed several issues concerning sensitivity, reliability, rapidness and the cost of chiral sensing platforms. Yet, research into chiral nanoprobes that can be used for visual monitoring of chiral substances is still in its infancy. As part of this study, a visual chiral recognition platform has been developed in which a combination of blue-emitting carbon dots (BCDs) and mercaptopropionic acid-capped CdTe quantum dots (MPA-QDs) with inherent chiroptical activity were... 

    Design and Development of Electrochemical DNA Nanobiosensors for Identification and Determination of Important Biomarkers

    , Ph.D. Dissertation Sharif University of Technology Salimian, Razieh (Author) ; Shahrokhian, Saeed (Supervisor) ; Kalhor, Hamid Reza (Co-Supervisor)
    Abstract
    The main purpose of this Ph.D. Thesis is to develop DNA-based biosensors using Label-free approaches applying simple, inexpensive and fast electrochemical techniques to measure the biological markers of cancer.In the first part, a simple protocol for detection of specific-sequence DNA is introduced. In this method carbon nanotube is used as a hybridization indicator. This label-free system provides the advantage of eliminating additional labeling procedure. As the signal enhances in the presence of MWCNT and decreases in the presence of target, the fabricated sensor is known as a signal-off device. The oxidation signal of Fe(CN)63-/4- is followed as an analytical signal to detect target... 

    Ultrasensitive detection of cancer biomarkers using conducting polymer/electrochemically reduced graphene oxide-based biosensor: Application toward BRCA1 sensing

    , Article Sensors and Actuators, B: Chemical ; Volume 266 , 2018 , Pages 160-169 ; 09254005 (ISSN) Shahrokhian, S ; Salimian, R ; Sharif University of Technology
    Elsevier B.V  2018
    Abstract
    Breast Cancer (BRCA) is the most common threat in women worldwide. Increasing death rate of diagnosed cases is the main leading cause of designing specific genosensors for BRCA − related cancer detection. In the present study, an ultrasensitive label − free electrochemical DNA (E − DNA) sensor based on conducting polymer/reduced graphene − oxide platform has been developed for the detection of BRCA1 gene. An electrochemical method was applied as a simple and controllable technique for the electrochemical reduction of graphene oxide and also, electro − polymerization of pyrrole − 3 − carboxylic acid monomer. The results of the present work show that the polymer − coated reduced graphene −... 

    The highly sensitive impedimetric biosensor in label free approach for hepatitis B virus DNA detection based on tellurium doped ZnO nanowires

    , Article Applied Physics A: Materials Science and Processing ; Volume 125, Issue 9 , 2019 ; 09478396 (ISSN) Khosravi Nejad, F ; Teimouri, M ; Jafari Marandi, S ; Shariati, M ; Sharif University of Technology
    Springer Verlag  2019
    Abstract
    The highly sensitive impedimetric biosensor in label free approach for hepatitis B virus DNA (HPV DNA) detection based on tellurium doped ZnO nanowires was fabricated. The NWs were grown by hybrid thin film oxidation in the physical vapor deposition (PVD) mechanism. The morphology characterization of the synthesized NWs was performed by field emission scanning electron microscopy (FESEM) and the images demonstrated that the diameter and the length of the materialized NWs were around 50 nm and several micrometers, respectively. The high-resolution transmission electron microscopy (HRTEM) image indicated that the fabricated NWs were crystalline and their phase characterization was validated by... 

    Microfluidic-based approaches in targeted cell/particle separation based on physical properties: fundamentals and applications

    , Article Small ; Volume 16, Issue 29 , 2020 Nasiri, R ; Shamloo, A ; Ahadian, S ; Amirifar, L ; Akbari, J ; Goudie, M. J ; Lee, K ; Ashammakhi, N ; Dokmeci, M. R ; Di Carlo, D ; Khademhosseini, A ; Sharif University of Technology
    Wiley-VCH Verlag  2020
    Abstract
    Cell separation is a key step in many biomedical research areas including biotechnology, cancer research, regenerative medicine, and drug discovery. While conventional cell sorting approaches have led to high-efficiency sorting by exploiting the cell's specific properties, microfluidics has shown great promise in cell separation by exploiting different physical principles and using different properties of the cells. In particular, label-free cell separation techniques are highly recommended to minimize cell damage and avoid costly and labor-intensive steps of labeling molecular signatures of cells. In general, microfluidic-based cell sorting approaches can separate cells using “intrinsic”... 

    Enhanced electrochemical activity of a hollow carbon sphere/polyaniline-based electrochemical biosensor for HBV DNA marker detection

    , Article ACS Biomaterials Science and Engineering ; Volume 5, Issue 5 , 2019 , Pages 2587-2594 ; 23739878 (ISSN) Salimian, R ; Shahrokhian, S ; Panahi, S ; Sharif University of Technology
    American Chemical Society  2019
    Abstract
    Herein, we present a novel, simple, and ultrasensitive electrochemical DNA (E-DNA) sensor based on hollow carbon spheres (HCS) decorated with polyaniline (PANI). A thiolated 21-mer oligonucleotide, characteristic of HBV DNA, is immobilized via electrodeposited gold nanoparticles on HCS-PANI. Cyclic voltammetry (CV), differential pulse voltammetry (DPV), and electrochemical impedance spectroscopy (EIS) are used to characterize the electrochemical properties of the prepared nanocomposite. Scanning electron microscopy is employed to investigate the morphological texture of the fabricated modifier. An enhanced intrinsic signal of PANI is probed to evaluate the biosensing ability of the prepared... 

    Toward single-DNA electrochemical biosensing by graphene nanowalls

    , Article ACS Nano ; Volume 6, Issue 4 , March , 2012 , Pages 2904-2916 ; 19360851 (ISSN) Akhavan, O ; Ghaderi, E ; Rahighi, R ; Sharif University of Technology
    2012
    Abstract
    Graphene oxide nanowalls with extremely sharp edges and preferred vertical orientation were deposited on a graphite electrode by using electrophoretic deposition in an Mg 2+-GO electrolyte. Using differential pulse voltammetry (DPV), reduced graphene nanowalls (RGNWs) were applied for the first time, in developing an ultra-high-resolution electrochemical biosensor for detection of the four bases of DNA (G, A, T, and C) by monitoring the oxidation signals of the individual nucleotide bases. The extremely enhanced electrochemical reactivity of the four free bases of DNA, single-stranded DNA, and double-stranded DNA (dsDNA) at the surface of the RGNW electrode was compared to electrochemical... 

    ZnO nanoparticle/nanorod-based label-free electrochemical immunoassay for rapid detection of MMP-9 biomarker

    , Article Biochemical Engineering Journal ; Volume 164 , 2020 Shabani, E ; Abdekhodaie, M. J ; Mousavi, S. A ; Taghipour, F ; Sharif University of Technology
    Elsevier B.V  2020
    Abstract
    A label-free electrochemical biosensor was developed for the rapid detection of the matrix metalloproteinase 9 (MMP-9) biomarker on the basis of antibody immobilizing on the zinc oxide (ZnO) nanoparticle and ZnO nanorod electrodes. The charge transfer resistance (Rct) of the electrodes was used as the indicator for MMP-9 concentration, which was obtained through cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). The ZnO nanorod-based biosensor exhibited linear behavior in the MMP-9 concentration range of 1–1000 ng/ml, which is a wider range than the available concentration ranges for most of the conventional methods. The biosensor sensitivity was 32.5 μA/(decade × cm2)... 

    The field effect transistor DNA biosensor based on ITO nanowires in label-free hepatitis B virus detecting compatible with CMOS technology

    , Article Biosensors and Bioelectronics ; Volume 105 , 15 May , 2018 , Pages 58-64 ; 09565663 (ISSN) Shariati, M ; Sharif University of Technology
    Elsevier Ltd  2018
    Abstract
    In this paper the field-effect transistor DNA biosensor for detecting hepatitis B virus (HBV) based on indium tin oxide nanowires (ITO NWs) in label free approach has been fabricated. Because of ITO nanowires intensive conductance and functional modified surface, the probe immobilization and target hybridization were increased strongly. The high resolution transmission electron microscopy (HRTEM) measurement showed that ITO nanowires were crystalline and less than 50 nm in diameter. The single-stranded hepatitis B virus DNA (SS-DNA) was immobilized as probe on the Au-modified nanowires. The DNA targets were measured in a linear concentration range from 1fM to 10 µM. The detection limit of...