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Experimental investigation of poly-β-hydroxybutyrate production by azohydromonas lata: Kinetics and artificial neural network modeling
, Article Chemical Engineering Communications ; Volume 203, Issue 2 , 2016 , Pages 224-235 ; 00986445 (ISSN) ; Younesi, H ; Ardjmand, M ; Safe Kordi, A ; Yaghmaei, S ; Qaderi, H ; Sharif University of Technology
Taylor and Francis Ltd
2016
Abstract
Batch culture of Azohydromonas lata was investigated for the production of intracellular poly-b-hydroxybutyrate (PHB). In order to determine the C:N value of the culture media for maximizing the microbial productivity of PHB, different concentrations of glucose and ammonium chloride were used as carbon and nitrogen sources, respectively. The optimal temperature and shaking rate was obtained at 30_C and 180 rpm, respectively. The maximum intracellular PHB concentration obtained was 5.09 g/l, which was 20% (w/w) of the cell dry weight (CDW) after 72 h. Also, the synthesis of PHB was growth associated with the C:N ratio of 153.71. The maximum calculated Yp/s was 0.212 (gr/gr) and the specific...