Search for: lipases
Surfactant production by an enzymatic method, Article Enzyme and Microbial Technology ; Volume 33, Issue 1 , 2003 , Pages 33-37 ; 01410229 (ISSN) ; Alemzadeh, I ; Sharif University of Technology
Elsevier Inc 2003
Glucose was acylated with palmitic acid using two kinds of lipases. The reactions were performed in the presence of an organic solvent. Solvent effect and molecular sieve effect and also the effect of enzyme amount and influence of molar ratio of substrates on glucose palmitate production with lipase from Rhizomucor miehei (Palatase® M) were studied. Application of molecular sieves together with open system increased palmitic acid conversion in the reaction mixture. Glucose palmitate was obtained in 41.18% conversion in hexane at 40°C with Palatase® M. Glucose palmitate was also synthesized with lipase from Candida antarctica (Novozyme 435). Esterification of palmitic acid with glucose...
Study on Biodiesel Production Using Engineered Lipases, M.Sc. Thesis Sharif University of Technology ; Kazemeini, Mohammad
Biodiesel has drawn the attention of many researches to itself as a replacing source for crude oil, recently. Transesterification is one of the most popular methods used for biodiesel production. Enzyme transesterification is a more simple method in comparison with acidic or basic transesterification. However, the high price and low yield of enzyme transesterification has caused many obstacles in its application. Enzyme engineering has gained much attention as a way to improve enzyme performance. In this thesis, biodiesel production by a native enzyme named Bacillus thermocatenulatus, and two engineered-enzymes is studied. First, effective parameters and variables in biodiesel production by...
Evaluation of biodiesel production using lipase immobilized on magnetic silica nanocomposite particles of various structures, Article Biochemical Engineering Journal ; Volume 79 , 2013 , Pages 267-273 ; 1369703X (ISSN) ; Kazemeini, M ; Arpanaei, A ; Sharif University of Technology
Nonporous and mesoporous silica-coated magnetite cluster nanocomposites particles were fabricated with various silica structures in order to develop a desired carrier for the lipase immobilization and subsequent biodiesel production. Lipase from Pseudomonas cepacia was covalently bound to the amino-functionalized particles using glutaraldehyde as a coupling agent. The hybrid systems that were obtained exhibited high stability and easy recovery regardless of the silica structure, following the application of an external magnetic field. The immobilized lipases were then used as the recoverable biocatalyst in a transesterification reaction to convert the soybean oil to biodiesel with methanol....
Lipase catalyzed incorporation of conjugated linoleic acid by transesterification into sunflower oil applying immobilized lipase (Lipozyme TL IM and RM IM), Article International Journal of Engineering, Transactions A: Basics ; Volume 29, Issue 4 , 2016 , Pages 436-443 ; 17281431 (ISSN) ; Alemzadeh, I ; Sharif University of Technology
Conjugated Linoleic Acid (CLA), Glycerol (G) and sunflower oil blends with varying concentration were subjected to enzymatic esterification using a 1, 3-specific immobilized lipase. CLA was used as acyl due to its purported health benefits. The transesterified lipids were evaluated for free fatty acids (FFA) and composition of fatty acids by gas chromatography. Lipozyme RM IM is preferred for enzymatic esterification because of more CLA incorporation and less free fatty acids at the end of reaction than Lipozyme TL IM. Response surface methodology (RSM) was used to determine the effects of three variables Glycerol concentration, reaction temperature, and amount of enzyme on the lipase...
Application of Enzymatic Method for Production of Biofuel from Domestic Oily Seed Resources, M.Sc. Thesis Sharif University of Technology ; Kariminia, Hamid Reza ; Shayegan, Jalaleddin
The enzymatic transesterification of castor oil with methanol in a solvent free media is carried out using lipase catalyst. The influence of several operation conditions on the production of biodiesel was analyzed i.e. the amount of enzyme, molar ratio of methanol to castor oil, reaction temperature and reaction time. It is observed that with increase in molar ratio, reaction time and enzyme amount, the amount of product (biodiesel) increased. When reaction temperature increased, the amount of biodiesel increased firstly and then decreased. The optimum conditions for the reaction were as follows: enzyme amount 300 mg, molar ratio of methanol to oil 9:1 and temperature 55°C. The maximum...
Experimental Evaluation of Optimum Conditions for Biological Production of Biodiesel Using Castor Oil, M.Sc. Thesis Sharif University of Technology ; Kariminia, Hamid Reza
In this study, biodiesel production using enzymatic method for transestrification of castor oil was investigated. It was found that the yield of biodiesel production is affected by methanol to oil molar ratio, temperature, enzyme amount, water content and reaction time. Effective factors were determined first, using two-factorial design method. Methanol to oil molar ratio was found as the most effective factor. Response surface methodology coupled with central composite design was utilized for statistical modeling of enzymatic biodiesel production and the reaction time was found as a non-significant factor. In another attempt, three factors including methanol to oil molar ratio, temperature,...
Influence of phosphate anions on the stability of immobilized enzymes. Effect of enzyme nature, immobilization protocol and inactivation conditions, Article Process Biochemistry ; Volume 95 , August , 2020 , Pages 288-296 ; Carballares, D ; Morellon Sterling, R ; Siar, E. H ; Kashefi, S ; Chafiaa, M ; Arana Peña, S ; Rios, N. S ; Gonçalves, L. R. B ; Fernandez Lafuente, R ; Sharif University of Technology
Elsevier Ltd 2020
A destabilizing effect at pH 7 of sodium phosphate on several lipases immobilized via interfacial activation is shown in this work. This paper investigates if this destabilizing effect is extended to other inactivation conditions, immobilization protocols or even other immobilized enzymes (ficin, trypsin, β-galactosidase, β-glucosidase, laccase, glucose oxidase and catalase). As lipases, those from Candida antarctica (A and B), Candida rugosa and Rhizomucor miehei have been used. Results confirm the very negative effect of 100 mM sodium phosphate at pH 7.0 for the stability of all studied lipases immobilized on octyl agarose, while using glutaraldehyde-support the effect is smaller (still...
Improvement of Edible Oil by Interestrification Process by Lipase, M.Sc. Thesis Sharif University of Technology ; Alemzadeh, Iran ; Razavi, Jalil
Fats and oils are one of the main human food diet components that provide the necessary fatty acids for our body. So the fatty acids available in consuming oils are so important. In this research the mixture of palm and sunflower oils with different compounds: (80:20, 60:40, 50:50, 40:60, 20:80) were used for preparing free- trans shortening by interesterification. For this purpose a special stabilized lipase (Lipozyme TLIM) were used and performed the physicochemical properties: free fatty acids, peroxide value, iodine value, slip melting point, solid fat content, P-anisidine value, soap, oxidative stability at 110 0C, and the profile of fatty acids on the primitive blends and final samples...
Studying the Performance of Membrane Separation System in the Lipase Enzyme Production Process, M.Sc. Thesis Sharif University of Technology ; Molaei Dehkordi, Asghar ; Roosta Azad, Reza ; Musavi, Abbas
In this study, the polysulfon ultrafilteration membranes were prepared and applied to concentrate the Lipase enzymes produced in the laboratory and the commercial enzymes of Novozymes Company. Using Design Expert software. The effect of membrane preparation conditions including polysulfone and polyvinylpyrrolidone concentration and the solvent type were investigated. The prepared membranes were characterized by measuring of distilled water flux, enzyme liquor flux, and enzyme activity in permeate and the Retentate flows. The results showed that increasing of polysulfone concentration reduced the flux of distilled water and enzyme activity in permeate and enhanced the enzyme activity in the...
Optimization of Lipase Immobilization, M.Sc. Thesis Sharif University of Technology ; Aalemzadeh, Iran ; Kazemi, Akhtarolmolouk
In this study, Pseudomonas aeruginosa BBRC-10036 was used for lipase production. The organism secreted the enzyme extracellulary. First of all, effect of initial pH of the culture broth on lipase activity was studied in order to determine the optimum condition for lipase production. After production, this enzyme must be separated from culture and after that the enzyme must be purified for using in analysis and industry. Different methods are used for purification of the enzyme. In this research, first precipitation was used and then this lipase has been purified by Ion exchange Chromatography leading to 2.33- fold purification and 11.47% recovery. In precipitation by acetone, maximum...
Fabrication localized surface plasmon resonance sensor chip of gold nanoparticles and detection lipase-osmolytes interaction, Article Applied Surface Science ; Vol. 314, issue , 2014 , Pages 138-144 ; ISSN: 01694332 ; Hoornam, S ; Vesaghi, M. A ; Ranjbar, B ; Azizi, A ; Mobasheri, H ; Sharif University of Technology
Co-deposition of RF-sputtering and RF-PECVD from acetylene gas and Au target were used to prepare sensor chip of gold nanoparticles (Au NPs). Deposition conditions were optimized to reach a Localized Surface Plasmon Resonance (LSPR) sensor chip of Au NPs with particle size less than 10 nm. The RF power was set at 180 W and the initial gas pressure was set at 0.035 mbar. Transmission Electron Microscopy (TEM) images and Atomic Force Microscopy (AFM) data were used to investigate particles size and surface morphology of LSPR sensor chip. The Au and C content of the LSPR sensor chip of Au NPs was obtained from X-ray photoelectron spectroscopy (XPS). The hydrogenated amorphous carbon (a-C:H)...
Green biodiesel production from various plant oils using nanobiocatalysts under different conditions, Article Bioenergy Research ; Volume 13, Issue 2 , 2020 , Pages 552-562 ; Kazemeini, M ; Arpanaei, A ; Sharif University of Technology
The lipase-immobilized polyethyleneimine (PEI)- and polyacrylic acid (PAA)–coated magnetic silica nanocomposite particles (L-PEI-MS and L-PAA-MS, respectively) were prepared and applied at various transesterification reaction conditions. The reactions were carried out with soybean, sunflower, canola, and palm oils along with methanol or ethanol in the solvent-free and n-hexane systems. The highest fatty acid methyl ester (FAME) and fatty acid ethyl ester (FAEE) synthesis yields were obtained from the transesterification of palm oil, i.e., almost 7.7–10.2% higher than other oils. At the constant reaction conditions, the application of ethanol leads to higher (6.0–8.7%) reaction yields in...
Preparation of Polyelectrolyte-Coated Magnetic Silica Nanocomposite Particles and Investigation of their Application for Enzyme Immobilization and Biodiesel Production, Ph.D. Dissertation Sharif University of Technology ; Kazemeini, Mohammad ; Arpanaei, Ayyoob ; Singh, Gurvinder
In this study, polyelectrolyte-coated magnetic silica nanocomposites were prepared via the covalent attachment of polyelectrolytes onto the as-prepared magnetite-silica particles with core-shell structures and average diameter of 210±50 nm, and subsequently applied for the immobilization of lipase molecules. In this regard, polyethyleneimine (PEI) with two molecular weights of 10000 and 750000 and polyacrylic acid (PAA) with two molecular weights of 1800 and 100000 were utilized. The type and molecular weight of the polyelectrolyte molecules greatly impacted the concentration of binding sites on the particles surface and consequently on the lipase immobilization efficiency and loading...
Mechanisms of Conjugated Linoleic acid and Enriched Glycerides Production as Neutraceuticals Food, Ph.D. Dissertation Sharif University of Technology ; Alemzadeh, Iran ; Vossughi, Manochehr
Dietary CLA production for medicinal purposes needs a safe and selective process. Biological production methods meet this goal. In this study, the microbial species that was suitable for CLA production was selected; then the CLA produced by bioconversion methods was utilized to produce CLA-enriched triglycerides by trans-esterification. Also the biochemical kinetics of CLA production and esterification reactions to produce CLA-enriched triglycerides was studied. In the presence of two substrates, the kinetics of enzymatic reaction was studied. Then the factors influencing on the microbial yield and CLA production including reaction conditions (reaction time, cell concentration and substrate...
The Study of Function of Lipase Biocatalyst Immobilized on Silica Nanoparticles Used for Biodiesel Production
Sharif University of Technology
Superparamagnetic core/shell nonporous (S1) and core/shell/shell mesoporous (S2 and S3) nanocomposite magnetite/silica particles with a magnetite cluster core of 130±30 nm, a nonporous silica shell of 90±10 nm thickness, and a mesoporous silica shell of 70±15 nm thickness were prepared thorough a simple method. Mesoporous particles were prepared with two BJH pore sizes (2.44 for S2 and 3.76 nm for S3 particles, respectively). The fabricated S1, S2 and S3 particles present high saturation magnetization values of 20, 13 and 17 emu/g, respectively. As a biological application, the lipase from Pseudomonas cepacia was successfully immobilized onto the amino-functionalized nanocomposite particles...
Comparing the Performance of Centrifuge, Membrane Filter and Filter Press for Clarification of Lipase Enzyme, M.Sc. Thesis Sharif University of Technology ; Rousta Azad, Reza
Lipases (Triacyl Glycerol Acyl Hydrolase, EC.126.96.36.199) are multidimensional biocatalysts which have many applications in food industrials, dairy, fats and oils processing, detergents, synthesis of pure chemical and pharmaceutical materials, paper industrial and cosmetics. Generally, industrial enzymes are produced extracellularly, so their separation from a low-cost complex medium (usually used for production) is one of the cost stages in their production process. Filtration is the most economical method of separation in the production of enzymes, but due to the small size of colloidal particles and cell masses present in the fermentation environment, its use is subject to limitations. In...
Lipase immobilisation on magnetic silica nanocomposite particles: Effects of the silica structure on properties of the immobilised enzyme, Article Journal of Materials Chemistry ; Volume 22, Issue 17 , 2012 , Pages 8385-8393 ; 09599428 (ISSN) ; Kazemeini, M ; Tabandeh, F ; Arpanaei, A ; Sharif University of Technology
Uniformly sized superparamagnetic single-shell nonporous (S1) and double-shell mesoporous silica nanocomposite particles with ∼130 nm magnetite cluster cores are synthesised in this study. Mesoporous particles are prepared with two BJH pore sizes (2.44 and 3.76 nm, designated as S2 and S3 particles, respectively). Once the lipase was immobilised on particles, our results showed that the enzyme loading capacities of mesoporous structures, i.e. S2 and S3, are higher than that for nonporous particles (S1). Hydrolytic activity tests reveal that immobilised lipases retain about 90% of the free enzyme's activity. Furthermore, comparing to the free enzyme, the thermal stability of immobilised...
Purification and zymography of lipase from aspergillus Niger PTCC5010, Article International Journal of Engineering, Transactions B: Applications ; Volume 28, Issue 8 , 2015 , Pages 1115-1123 ; 1728-144X (ISSN) ; Alemzadeh, I ; Tabatabaee Yazdi, F ; Vossoughi, M ; Varidi, M ; Sharif University of Technology
Materials and Energy Research Center 2015
In this study, Lipase from Aspergillus Niger after extraction of medium culture was precipitated with different percentages of acetone and purified by ion exchange chromatography using SP-Sepharose HP and Q-Sepharose HP. The process of purification of the enzyme was studied by electrophoresis and the molecular weight was detected and determined by Zymography using overlying containing phenol red and Rhodamine B. The results showed that the vast majority of lipase from this strain has been precipitated by 70% saturation acetone, and leads to the 1.67 fold the purified enzyme, with special activities 32.8 U. mg-1 and efficiency of 38.5%. Using two-phase chromatography, enzyme specific activity...
Amine-functionalized magnetic nanocomposite particles for efficient immobilization of lipase: effects of functional molecule size on properties of the immobilized lipase, Article RSC Advances ; Volume 5, Issue 42 , Apr , 2015 , Pages 33313-33327 ; 20462069 (ISSN) ; Kazemeini, M ; Singh, G ; Arpanaei, A ; Sharif University of Technology
Royal Society of Chemistry 2015
A cost-effective design of reusable enzyme-functionalized particles with better catalytic activity is of great scientific interest due to their applications in a wide range of catalytic reactions in several industrial processes. In this work, a systematic approach for preparing amine-functionalized magnetic nanocomposite particles through the surface modification of core/shell type Fe
3O 4 cluster@SiO 2 particles by the small molecules of 3-(2-aminoethyl)aminopropyltrimethoxysilane (AAS) or the large molecules of polyethyleneimine (PEI) with two different molecular weights, as the support materials for enzyme immobilization, has been demonstrated. The functional...
Study of molecular conformation and activity-related properties of lipase immobilized onto core-shell structured polyacrylic acid-coated magnetic silica nanocomposite particles, Article Langmuir ; Volume 32, Issue 13 , 2016 , Pages 3242-3252 ; 07437463 (ISSN) ; Kazemeini, M ; Singh, G ; Arpanaei, A ; Sharif University of Technology
American Chemical Society
A facile approach for the preparation of core-shell structured poly(acrylic acid) (PAA)-coated Fe3O4 cluster@SiO2 nanocomposite particles as the support materials for the lipase immobilization is reported. Low- or high-molecular-weight (1800 and 100 000, respectively) PAA molecules were covalently attached onto the surface of amine-functionalized magnetic silica nanoacomposite particles. The successful preparation of particles were verified by scanning transmission electron microscopy (STEM), X-ray diffraction (XRD), vibrating sample magnetometer (VSM), thermogravimetric analysis (TGA), zeta potential measurement, and Fourier-transform infrared (FTIR) techniques. Once lipase is covalently...