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    Growth kinetic Analysis of Biosurfactant Producing Bacteria for Oil Reservoir Enhance Oil Recovery

    , M.Sc. Thesis Sharif University of Technology Maghsoudi, Ehsan (Author) ; Roosta Azad, Reza (Supervisor) ; Yaghmaei, Soheyla (Supervisor)
    Abstract
    Evidently, environment contamination by water insoluble chemicals present at the industrial wastes could bring about catastrophic consequences to the biosphere. Biological based cleaning technologies are commonly considered as a practical remedy to remove these contaminations. In fact, these techniques are founded on the ability of certain microorganisms to degrade the contaminants. It is crystal clear that the availability of contaminants to the microorganisms is an essential parameter determining the final efficiency of the biodegradation process. In practice, the limited solubility of the contaminants in the aqueous phase leads to mass transfer constraints which consequently, decrease the... 

    Interactive optimization of biosurfactant production by Paenibacillus alvei ARN63 isolated from an Iranian oil well

    , Article Colloids and Surfaces B: Biointerfaces ; Volume 82, Issue 1 , 2011 , Pages 33-39 ; 09277765 (ISSN) Najafi, A. R ; Rahimpour, M. R ; Jahanmiri, A. H ; Roostaazad, R ; Arabian, D ; Soleimani, M ; Jamshidnejad, Z ; Sharif University of Technology
    Abstract
    The potential of an indigenous bacterial strain isolated from an Iranian oil field for the production of biosurfactant was investigated in this study. After isolation, the bacterium was characterized to be Paenibacillus alvei by biochemical tests and 16S ribotyping. The biosurfactant, which was produced by this bacterium, was able to lower the surface tension of media to 35. mN/m. Accordingly, thin layer chromatography (TLC) and FT-IR has been carried out to determine compositional analysis of the produced biosurfactant. After all the tests related to characterization of the biosurfactant produced by the isolated bacterium, it was characterized as lipopeptide derivative. The combination of... 

    Purification and characterization of a novel biosurfactant produced by Bacillus licheniformis MS3

    , Article World Journal of Microbiology and Biotechnology ; Volume 26, Issue 5 , 2010 , Pages 871-878 ; 09593993 (ISSN) Biria, D ; Maghsoudi, E ; Roostaazad, R ; Dadafarin, H ; Sahebghadam Lotfi, A ; Amoozegar, M. A ; Sharif University of Technology
    2010
    Abstract
    The physical properties and chemical structure of a new biosurfactant (licheniformin) produced by Bacillus licheniformis MS3 were investigated. The purified biosurfactant was identified as a lipopeptide with amino acid sequence of Gly, Ala, Val, Asp, Ser, Gly, Tyr and a lactone linkage between the carboxyl group of Aspargine and hydroxyl group of Tyrosine residue. The fatty acid moiety was attached to N-terminal amino acid residue through an amide bond. The purified licheniformin could lower the surface tension of water from 72 to 38 mN/m at concentrations higher than 15 μg/mL and its relative emulsion volume (EV%) was equal to 36%. It also showed stable surface activity over a wide range of... 

    Genome annotation and comparative genomic analysis of Bacillus subtilis MJ01, a new bio-degradation strain isolated from oil-contaminated soil

    , Article Functional and Integrative Genomics ; Volume 18, Issue 5 , 2018 , Pages 533-543 ; 1438793X (ISSN) Rahimi, T ; Niazi, A ; Deihimi, T ; Taghavi, S. M ; Ayatollahi, S ; Ebrahimie, E ; Sharif University of Technology
    Springer Verlag  2018
    Abstract
    One of the main challenges in elimination of oil contamination from polluted environments is improvement of biodegradation by highly efficient microorganisms. Bacillus subtilis MJ01 has been evaluated as a new resource for producing biosurfactant compounds. This bacterium, which produces surfactin, is able to enhance bio-accessibility to oil hydrocarbons in contaminated soils. The genome of B. subtilis MJ01 was sequenced and assembled by PacBio RS sequencing technology. One big contig with a length of 4,108,293 bp without any gap was assembled. Genome annotation and prediction of gene showed that MJ01 genome is very similar to B. subtilis spizizenii TU-B-10 (95% similarity). The comparison... 

    Enhancing biosurfactant production from an indigenous strain of Bacillus mycoides by optimizing the growth conditions using a response surface methodology

    , Article Chemical Engineering Journal ; Volume 163, Issue 3 , October , 2010 , Pages 188-194 ; 13858947 (ISSN) Najafi, A. R ; Rahimpour, M. R ; Jahanmiri, A. H ; Roostaazad, R ; Arabian, D ; Ghobadi, Z ; Sharif University of Technology
    2010
    Abstract
    In this study, we have investigated the potential of a native bacterial strain isolated from an Iranian oil field for the production of biosurfactant. The bacterium was identified to be Bacillus mycoides by biochemical tests and 16S ribotyping. The biosurfactant, which was produced by this bacterium, was able to reduce the surface tension of media to 34. mN/m. Compositional analysis of the produced biosurfactant has been carried out by thin layer chromatography (TLC) and FT-IR. The biosurfactant produced by the isolate was characterized as lipopeptide derivative. Biosurfactant production was optimized by the combination of central composite design (CCD) and response surface methodology...