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    Design, Fabrication and Testing of an Integrated Microfluidic Platform for Water Quality Testing

    , M.Sc. Thesis Sharif University of Technology Mortazavi, Mohammad Ali (Author) ; Saeedi, Mohammad Saeed (Supervisor)
    Nowadays environmental issues are of great importance and one of the most paramount issues is the quality of water, because clean water is vital to our existence. Besides different methods for water purification, water quality testing is a useful and needful tool. Traditional methods for determination of water quality parameters are mainly expensive, time consuming and require highly trained staff. In this study, we have described a centrifugal microfluidic platform (Lab- on – a- CD) for simultaneous determination of different physical and chemical parameters including Nitrite, Free Chlorine and pH. All of the processes from sample injection to detection are integrated on this low-cost and... 

    Micro-Nanofluidic Isolation of Circulating Exosomes from Blood Plasma

    , M.Sc. Thesis Sharif University of Technology Abdorahimzadeh, Amir Hossein (Author) ; Taghipoor, Mojtaba (Supervisor) ; saeedi, Mohammad saeed (Supervisor)
    Exosomes are a type of extracellular vesicles which are secreted by cells and could be found in all of human biofluids including blood. The size of exosomes is in the range of 30 – 150 nm. And they have a spherical structure with a phospholipid membrane. Exosomes are very important in academic research for the purpose of diagnostic and therapeutic practices because of their protein and nucleic acid contents.In this study a technological system was developed for isolation of circulating exosomes of human whole blood. This system consisted of a collection of microfluidic chips and protocols in order to extract a purified sample of exosomes from whole blood. First, a chip based on centrifugal... 

    Fabrication of a Centrifugal Microfluidic System for Cell-free DNA Isolation from whole Blood as a Biomarker

    , M.Sc. Thesis Sharif University of Technology Hatami, Ali (Author) ; Saadatmand, Maryam (Supervisor) ; Garshasbi, Masoud (Co-Supervisor)
    Today, medical science has come to the conclusion that the best time to start treating a disease is in its early stages. Therefore, the topic of early detection has been widely welcomed in scientific circles. Today, one of the safest methods for early detection in medicine is the study of biomarkers. These biomarkers include CTC, cfRNA, cfDNA, Exosome, etc. For instance, in the discussion of early cancer detection, the US Food and Drug Administration has so far identified two biomarkers, cfDNA and CTC, as the safest markers. The cfDNA marker is also widely used not only in the diagnosis of cancer, but also in the field of sex diagnosis and fetal Down syndrome in the first weeks of pregnancy.... 

    Design and Fabrication of a Centrifugal Microfluidic System to Cell Lysis

    , M.Sc. Thesis Sharif University of Technology Khorrami Jahromi, Arash (Author) ; Saadatmand, Maryam (Supervisor) ; Eghbal, Manouchehr (Supervisor) ; Parsa Yeganeh, Laleh (Co-Supervisor)
    Cell lysis, as the first procedure of cell pretreatment, is a process that breaks cell membranes open thereby facilitating access to intracellular substances, such as DNA, proteins, and other components for further analysis to diagnose and treat diseases at early stages. Currently, there are several methods for cell lysis at macroscales. However, the time-consuming and expensive procedures as well as the large scal of the system are the main disadvantages of the systems. Recently, microfluidic systems have attracted considerable attention due to advantages associated with automation, integration and miniaturization of biomedical test protocols. Centrifugal microfluidics (Lab-on-a-Disc) is a... 

    Design and Fabrication of a Centrifugal Microfluidic System to DNA Extraction

    , M.Sc. Thesis Sharif University of Technology Fathi Ganje Lou, Ali (Author) ; Farhadi, Fathollah (Supervisor) ; Saadatmand, Maryam (Supervisor) ; Parsa Yeganeh, Laleh (Co-Supervisor)
    Deoxyribonucleic acid (DNA) extraction, as one of the most important steps in modern molecular diagnostics, is the process by which DNA is separated from intracellular materials like proteins, membranes, and other materials contained in the cell. Microfluidic technology enables sophisticated, time-consuming and costly experiments with minimal use of raw materials, time and cost and acceptable accuracy. The predominant advantages of centrifugal microfluidic systems are utilizing centrifugal force to generate propulsion without the need for a pump, and eliminating the need for experts to run the system. Various fluidic operations such as valving, mixing, metering, heating, and sample... 

    Design and Fabrication of Centrifugal Microfluidic Disc for Cell Lysis and DNA Extraction

    , M.Sc. Thesis Sharif University of Technology Jalalian, Pourya (Author) ; Saadatmand, Maryam (Supervisor) ; Garshasbi, Masoud (Co-Supervisor)
    Centrifugal microfluidic systems due to their unique properties, including the ease of sample transfer, high controllability, the need for samples and reagents in a small volume scale, the possibility of embedding a complete laboratory and modeling a variety of processes on a disk, they have great potential to be used in the field of point of care diagnosis. Such devices can perform several different processes simultaneously and continuously by using inertial and pneumatic forces as well as a complex network of microchannels. The purpose of this research is to design and build a centrifugal microfluidic disc for lysing amniotic stem cells, followed by DNA extraction, in order to detect fetal... 

    Newtonian and generalized Newtonian reacting flows in serpentine microchannels: pressure driven and centrifugal microfluidics

    , Article Journal of Non-Newtonian Fluid Mechanics ; Volume 251 , January , 2018 , Pages 88-96 ; 03770257 (ISSN) Madadelahi, M ; Shamloo, A ; Sharif University of Technology
    Elsevier B.V  2018
    This paper presents a comprehensive 3D numerical simulation of reacting flows in micro scale dimension through centrifugal, or Lab-On-a-CD (LOCD), and pressure-driven, or Lab-On-a-Chip (LOC) devices. Three different serpentine channel configurations (rectangular, triangular and sinusoidal) are investigated. In these configurations, two chemical species enter from two inlets and according to an irreversible chemical reaction, start yielding other species. Both Newtonian and generalized Newtonian fluids are considered in the simulations and the results are compared for both LOC and LOCD devices. Besides, the effects of different parameters such as the aspect ratio of channels’ cross section,...