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Measurement of Partition Coefficient of L-Lysine HCl Amino Acid in Polymer-Salt Aqueous Two-Phase Systems
, M.Sc. Thesis Sharif University of Technology ; Vossoughi, Manouchehr (Supervisor) ; Alamzadeh, Eiran (Supervisor)
Abstract
The partition coefficients of L-Lysine HCL were measured in polymer-salt aqueous two-phase systems. These systems contain polyethylene glycol with nominal molecular weight of 4000 or 10000 and two different salts (di-potassium hydrogen phosphate or tri-sodium citrate 5,5-hydrate). The experimental data were obtained at temperatures 293.15, 298.15 and 303.15 K. The effects of temperature, pH, polymer and salt concentrations, polymer molecular weight and salt type on the partitioning of L-Lysine HCL were also studied. The results showed that salt concentration has a significant effect on the partition coefficient while temperature has a low effect. The experimental partition coefficients of...
Application of Cellulose Nanofibers Coated Quartz Crystal Microbalance (QCM) Biosensor for Amino Acid Detection in Aqueous Media
, Ph.D. Dissertation Sharif University of Technology ; Irajizad, Azam (Supervisor) ; Vossoughi, Manouchehr (Supervisor)
Abstract
Developing a simple, cost effective and accurate detection method for L-lysine (Lys), L-leucine (Leu) and glycine (Gly) as the important analytes in clinical diagnostics, biological processes and food industries is of great interest. Therefore, in the first part of this research, cellulose nanofibrils (CNFs) were coated on a quartz crystal microbalance (QCM) surface by spin coating to achieve a QCM biodetector for Gly. Thus, the two-layer CNFs coating was selected as sensing film and was applied for following experiments. In the next step, the coated QCMs were carefully characterized before and after interaction with Gly using water contact angle (WCA), Fourier transform infrared...
Synthesis of Red Phosphorus-Glycerol Copolymer and its Covalent Binding to Lysozyme Enzyme to Investigate the Structure and Function of the Enzyme within the Complex and CQD-Catalyzed Synthesis of Formamide Compounds
, M.Sc. Thesis Sharif University of Technology ; Kalhor, Hamid Reza (Supervisor)
Abstract
Red phosphorus, due to its unique chemical properties, and glycidol, with its reactive functional groups, provide an effective combination for synthesizing new copolymers with potential applications in biological fields, particularly in enzyme stabilization. In this study, a novel copolymer was synthesized using red phosphorus and glycidol through an anionic ring-opening mechanism. The synthesis process was confirmed using various analytical techniques, including FTIR, NMR, and thermal analyses, which demonstrated the successful incorporation of functional groups and an average phosphorus content of 40% in the copolymer. In the next step, modification of this copolymer by linking it to the...