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Speed control of a digital servo system using brain emotional learning based intelligent controller
, Article PEDSTC 2013 - 4th Annual International Power Electronics, Drive Systems and Technologies Conference ; 2013 , Pages 311-314 ; 9781467344845 (ISBN) ; Shahri, A. M ; Shuraki, S. B ; Sharif University of Technology
2013
Abstract
In this paper, a biologically motivated controller based on a mammalian limbic system called Brain Emotional Learning Based Intelligent Controller (BELBIC) is used for speed control of a Digital Servo System. The proposed controller is applied experimentally to a laboratory Digital Servo System 1 via MATLAB external mode. Comparing results of the proposed controller with conventional PID controller shows satisfactory performance including faster response and lower overshoot
Dose-dependent effects of nanoscale graphene oxide on reproduction capability of mammals
, Article Carbon ; Volume 95 , December , 2015 , Pages 309-317 ; 00086223 (ISSN) ; Ghaderi, E ; Hashemi, E ; Akbari, E ; Sharif University of Technology
Elsevier Ltd
2015
Abstract
In vivo dose-dependent effects of nanoscale graphene oxide (NGO) sheets on reproduction capability of Balb/C mice were investigated. Biodistribution study of the NGO sheets (intravenously injected into male mice at dose of ∼2000 μg/mL or 4 mg/kg of body weight) showed a high graphene uptake in testis. Hence, in vivo effects of the NGO sheets on important characteristics of spermatozoa (including their viability, morphology, kinetics, DNA damage and chromosomal aberration) were evaluated. Significant in vivo effects was found at the injected concentrations ≥200 μg/mL after (e.g., ∼45% reduction in sperm viability and motility at 2000 μg/mL). Observation of remarkable DNA fragmentations and...
Toward chemical perfection of graphene-based gene carrier via ugi multicomponent assembly process
, Article Biomacromolecules ; Volume 17, Issue 9 , 2016 , Pages 2963-2971 ; 15257797 (ISSN) ; Akhavan, O ; Hashemi, E ; Shamsara, M ; Sharif University of Technology
American Chemical Society
Abstract
The graphene-based materials with unique, versatile, and tunable properties have brought new opportunities for the leading edge of advanced nanobiotechnology. In this regard, the use of graphene in gene delivery applications is still at early stages. In this study, we successfully designed a new complex of carboxylated-graphene (G-COOH) with ethidium bromide (EtBr) and used it as a nanovector for efficient gene delivery into the AGS cells. G-COOH, with carboxyl functions on its surface, in the presence of EtBr, formaldehyde, and cyclohexylisocyanide were participated in Ugi four component reaction to fabricate a stable amphiphilic graphene-EtBr (AG-EtBr) composite. The coupling reaction was...
Dielectrophoretic cell sorting via sliding cells on 3D silicon microelectrodes
, Article Proceedings of the IEEE International Conference on Micro Electro Mechanical Systems (MEMS), 22 January 2017 through 26 January 2017 ; 2017 , Pages 147-150 ; 10846999 (ISSN) ; 9781509050789 (ISBN) ; Chan, M. L ; Roshan, K. A ; Yobas, L ; Sharif University of Technology
Abstract
This work presents an innovative design for a flow-through dielectrophoretic cell sorting based on silicon bulk microelectrodes featuring sidewall undercuts. The microelectrodes are configured into an interdigitated array with digits extending across the flow chamber at an oblique angle against the flow stream. Target cells under dielectrophoretic forces and hydrodynamic drag can slide along the digits to a dedicated outlet. The design has been showcased for continuous-flow sorting of viable and non-viable mammalian cells, achieving a throughput of 16,600 cells/min, an order of magnitude higher than those reported for existing continuous-flow cell sorting designs using thin-film or...
Attitude control of a quadrotor using brain emotional learning based intelligent controller
, Article 13th Iranian Conference on Fuzzy Systems, IFSC 2013 ; 2013 ; Shahri, A. M ; Shouraki, S. B ; Sharif University of Technology
IEEE Computer Society
2013
Abstract
For the first time in this paper, Brain Emotional Learning Based Intelligent Controller (BELBIC) is applied to attitude control of a Quadrotor. BELBIC controller is designed based on the computational model of emotional learning process in mammalian brain limbic system. Proposed control algorithm is employed because of the learning ability and independency to system model and also satisfactory performances dealing with disturbances and changing in system parameters. Quadrotor is an Unmanned Aerial Vehicle (UAV) which has the capability of Vertical Take-Off and Landing (VTOL). Simulation results of controlling Quadrotor with BELBIC are addressed. Also pitch angle disturbance is applied due to...
Whole cell mechanical property characterization based on mechanical properties of its cytoplasm and bio membrane
, Article ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE), 9 November 2012 through 15 November 2012 ; Volume 2 , November , 2012 , Pages 545-551 ; 9780791845189 (ISBN) ; Ahmadian, M. T ; Sharif University of Technology
2012
Abstract
Analysis and investigation of the relation between different parts of biological cells such as biomembrane, cytoplasm and nucleus can help to better understand their behaviors and material properties. In this paper, first, the whole elastic properties of mouse oocyte and embryo cells have been computed by inverse finite element and Levenberg-Marquardt optimization algorithm and second, using the derived mechanical properties and the mechanical properties of its bio membrane from the literature, the mechanical properties of its cytoplasm has been characterized. It has been assumed that the cell behavior is as continues, isotropic, nonlinear and homogenous material for modeling. Matching the...
Deformation characterization of mouse oocyte cell using inverse finite element and LevenbergâMarquardt optimization algorithm in needle injection experiment
, Article Proceedings of the ASME Design Engineering Technical Conference, 12 August 2012 through 12 August 2012 ; Volume 2, Issue PARTS A AND B , August , 2012 , Pages 847-852 ; 9780791845011 (ISBN) ; Ahmadian, M. T ; Sharif University of Technology
2012
Abstract
In order to better understand the mechanical properties of biological cells, characterization and investigation of their material behavior is necessary. In this paper hyperelastic Neo-Hookean material is used to characterize the mechanicalproperties of mouse oocyte cell. It has been assumed that the cell behavior is continues, isotropic, nonlinear and homogenous material. Then, by matching the experimental data with finite element (FE) simulation result and using the LevenbergâMarquardt optimization algorithm, the nonlinear hyperelastic model parameters have been extracted. Experimental data of mouse oocyte captured from literatures. Advantage of the developed model is that it can be used to...
Modeling of cell deformation under external force using artificial neural network
, Article ASME International Mechanical Engineering Congress and Exposition, Proceedings (IMECE), 12 November 2010 through 18 November 2010 ; Volume 2 , 2010 , Pages 659-665 ; 9780791844267 (ISBN) ; Vossoughi, G. R ; Abbasi, A. A ; Raeissi, P ; Sharif University of Technology
Abstract
Embryogenesis, regeneration and cell differentiation in microbiological entities are influenced by mechanical forces. Therefore, development of mechanical properties of these materials is important. Neural network technique is a useful method which can be used to obtain cell deformation by the means of force-geometric deformation data or vice versa. Prior to insertion in the needle injection process, deformation and geometry of cell under external point-load is a key element to understand the interaction between cell and needle. In this paper the goal is the prediction of cell membrane deformation under a certain force, and to visually estimate the force of indentation on the membrane from...
Influence of heavy nanocrystals on spermatozoa and fertility of mammals
, Article Materials Science and Engineering C ; Volume 69 , 2016 , Pages 52-59 ; 09284931 (ISSN) ; Hashemi, E ; Zare, H ; Shamsara, M ; Taghavinia, N ; Heidari, F ; Sharif University of Technology
Elsevier Ltd
Abstract
In recent years, quantum dots (QDs) have been widely used in upcoming nanotechnology-based solar cells, light-emitting diodes and even bioimaging, due to their tunable optical properties and excellent quantum yields. But, such nanostructures are currently constituted by heavy elements which can threat the human health and living environment. Hence, in this work, the in vivo effects of CdTe nanocrystals (NCs) (as one of the promising QDs) on spermatozoa of male mice and subsequently on fertility of female mice were investigated, for the first time. To do this, CdTe NCs were synthesized through an environment-friendly (aqueous-based solution) method. The sperm cells presented a high potential...
High antimicrobial activity and low human cell cytotoxicity of core-shell magnetic nanoparticles functionalized with an Antimicrobial Peptide
, Article ACS Applied Materials and Interfaces ; Volume 8, Issue 18 , 2016 , Pages 11366-11378 ; 19448244 (ISSN) ; Rai, A ; Pinto, S ; Evangelista, M ; Cardoso, R. M. S ; Paulo, C ; Carvalheiro, T ; Paiva, A ; Imani, M ; Simchi, A ; Durães, L ; Portugal, A ; Ferreira, L ; Sharif University of Technology
American Chemical Society
2016
Abstract
Superparamagnetic iron oxide nanoparticles (SPIONs) functionalized with antimicrobial agents are promising infection-targeted therapeutic platforms when coupled with external magnetic stimuli. These antimicrobial nanoparticles (NPs) may offer advantages in fighting intracellular pathogens as well as biomaterial-associated infections. This requires the development of NPs with high antimicrobial activity without interfering with the biology of mammalian cells. Here, we report the preparation of biocompatible antimicrobial SPION@gold core-shell NPs based on covalent immobilization of the antimicrobial peptide (AMP) cecropin melittin (CM) (the conjugate is named AMP-NP). The minimal inhibitory...
Proposing a high-efficiency dielectrophoretic system for separation of dead and live cells
, Article Scientia Iranica ; Volume 25, Issue 1 , 2018 , Pages 186-195 ; 10263098 (ISSN) ; Nassiri Nazif, K ; Soufi, A. M ; Saidi, M. S ; Sharif University of Technology
Sharif University of Technology
2018
Abstract
Recently, electrode-based Dielectrophoresis (eDEP) has been used for particle manipulation by means of triangular electrodes. In this theoretical and numerical study, a microchannel with quarter-of-ellipse electrodes is presented and a detailed comparison with triangular electrodes is provided. Electric field, resultant DEP force, and particle trajectories for each microchannel are evaluated by means of COMSOL Multiphysics 4.4. Afterwards, focusing and separation efficiencies of the systems are assessed and compared. Finally, separation efficiency of our proposed model for live and dead cells is compared with that of our previous model published in the literature [1]. It is demonstrated that...
A portable culture chamber for studying the effects of hydrostatic pressure on cellular monolayers
, Article Proceedings of the Institution of Mechanical Engineers, Part C: Journal of Mechanical Engineering Science ; 2018 ; 09544062 (ISSN) ; Saidi, M ; Mosadegh, B ; Firoozabadi, B ; Sharif University of Technology
SAGE Publications Ltd
2018
Abstract
Hydrostatic pressure is one of the most fundamental and common mechanical stimuli in the body, playing a critical role in the homeostasis of all organ systems. Kidney function is affected by high blood pressure, namely hypertension, by the increased pressure acting on the glomerular capillary walls. This general effect of hypertension is diagnosed as a chronic disease, but underlying mechanistic causes are still not well understood. This paper reports a portable and adaptive device for studying the effects of hydrostatic pressure on a monolayer of cells. The fabricated device fits within a conventional incubation system and microscope. The effects of various pressures and durations were...
A portable culture chamber for studying the effects of hydrostatic pressure on cellular monolayers
, Article Proceedings of the Institution of Mechanical Engineers, Part C: Journal of Mechanical Engineering Science ; Volume 233, Issue 3 , 2019 , Pages 807-816 ; 09544062 (ISSN) ; Saidi, M. S ; Mosadegh, B ; Firoozabadi, B ; Sharif University of Technology
SAGE Publications Ltd
2019
Abstract
Hydrostatic pressure is one of the most fundamental and common mechanical stimuli in the body, playing a critical role in the homeostasis of all organ systems. Kidney function is affected by high blood pressure, namely hypertension, by the increased pressure acting on the glomerular capillary walls. This general effect of hypertension is diagnosed as a chronic disease, but underlying mechanistic causes are still not well understood. This paper reports a portable and adaptive device for studying the effects of hydrostatic pressure on a monolayer of cells. The fabricated device fits within a conventional incubation system and microscope. The effects of various pressures and durations were...
Estimation of mean radius, length and density of microvasculature using diffusion and perfusion MRI
, Article Scientia Iranica ; Volume 13, Issue 4 , 2006 , Pages 348-354 ; 10263098 (ISSN) ; Jahed, M ; Chopp, M ; Mireshghi, A ; Sharif University of Technology
Sharif University of Technology
2006
Abstract
In theory, diffusion and perfusion information in MRI maps can be combined to yield morphological information, such as capillary density, volume and possibly capillary plasma velocity. This paper suggests a new method for determination of mean radius, length and capillary density in normal regions using diffusion and perfusion MRI. Mean Transit Time (MTT), Cerebral Blood Volume (CBV), Apparent Diffusion Coefficient (ADC), pseudo-diffusion coefficient (D*) and ΔR2 and ΔR2* values were utilized to calculate mean radius, length and capillary density. To verify the proposed theory, a special protocol was designed and tested on normal regions of a male Wistar rat using obtained functions. Mean...
Development of ultrasensitive biomimetic auditory hair cells based on piezoresistive hydrogel nanocomposites
, Article ACS Applied Materials and Interfaces ; Volume 13, Issue 37 , 2021 , Pages 44904-44915 ; 19448244 (ISSN) ; Moradi, H ; Pastras, C. J ; Abolpour Moshizi, S ; Wu, S ; Asadnia, M ; Sharif University of Technology
American Chemical Society
2021
Abstract
With an ageing population, hearing disorders are predicted to rise considerably in the following decades. Thus, developing a new class of artificial auditory system has been highlighted as one of the most exciting research topics for biomedical applications. Herein, a design of a biocompatible piezoresistive-based artificial hair cell sensor is presented consisting of a highly flexible and conductive polyvinyl alcohol (PVA) nanocomposite with vertical graphene nanosheets (VGNs). The bilayer hydrogel sensor demonstrates excellent performance to mimic biological hair cells, responding to acoustic stimuli in the audible range between 60 Hz to 20 kHz. The sensor output demonstrates stable...
Development of ultrasensitive biomimetic auditory hair cells based on piezoresistive hydrogel nanocomposites
, Article ACS Applied Materials and Interfaces ; Volume 13, Issue 37 , 2021 , Pages 44904-44915 ; 19448244 (ISSN) ; Moradi, H ; Pastras, C. J ; Abolpour Moshizi, S ; Wu, S ; Asadnia, M ; Sharif University of Technology
American Chemical Society
2021
Abstract
With an ageing population, hearing disorders are predicted to rise considerably in the following decades. Thus, developing a new class of artificial auditory system has been highlighted as one of the most exciting research topics for biomedical applications. Herein, a design of a biocompatible piezoresistive-based artificial hair cell sensor is presented consisting of a highly flexible and conductive polyvinyl alcohol (PVA) nanocomposite with vertical graphene nanosheets (VGNs). The bilayer hydrogel sensor demonstrates excellent performance to mimic biological hair cells, responding to acoustic stimuli in the audible range between 60 Hz to 20 kHz. The sensor output demonstrates stable...
Graphene nanomesh promises extremely efficient in vivo photothermal therapy
, Article Small ; Volume 9, Issue 21 , 2013 , Pages 3593-3601 ; 16136810 (ISSN) ; Ghaderi, E ; Sharif University of Technology
2013
Abstract
Reduced graphene oxide nanomesh (rGONM), as one of the recent structures of graphene with a surprisingly strong near-infrared (NIR) absorption, is used for achieving ultraefficient photothermal therapy. First, by using TiO2 nanoparticles, graphene oxide nanoplatelets (GONPs) are transformed into GONMs through photocatalytic degradation. Then rGONMs functionalized by polyethylene glycol (PEG), arginine-glycine-aspartic acid (RGD)-based peptide, and cyanine 7 (Cy7) are utilized for in vivo tumor targeting and fluorescence imaging of human glioblastoma U87MG tumors having ανβ3 integrin receptors, in mouse models. The rGONM-PEG suspension (1 μg mL -1) exhibits about 4.2- and 22.4-fold higher NIR...
Functional analyses of recombinant mouse hepcidin-1 in cell culture and animal model
, Article Biotechnology Letters ; Volume 35, Issue 8 , August , 2013 , Pages 1191-1197 ; 01415492 (ISSN) ; Keyhanvar, N ; Kalhor, H. R ; Rezaei, A ; Sharif University of Technology
2013
Abstract
Hepcidin is a peptide hormone that plays an important role in iron metabolism. We have produced a recombinant mouse hepcidin-1 by using baculovirus expression system. Its expression yield was 25 μg/ml when cell culture media were supplemented with a protease inhibitor cocktail. The recombinant mouse hepcidin-1 and synthetic human hepcidin-25 had similar effects on reducing ferroportin expression in J774A cell line and in peritoneal macrophages. However, synthetic human hepcidin-25 was more efficient than recombinant mouse hepcidin-1 in reducing iron concentration in blood circulation (p < 0.01)
Evaluating the effect of ultrasmall superparamagnetic iron oxide nanoparticles for a long-term magnetic cell labeling
, Article Journal of Medical Physics ; Volume 38, Issue 1 , 2013 , Pages 34-40 ; 09716203 (ISSN) ; Oghabian, M. A ; Allen, B. J ; Amanlou, M ; Masoudi, A ; Daha, F. J ; Sharif University of Technology
2013
Abstract
In order to evaluate the long-term viability, the iron content stability, and the labeling efficiency of mammalian cells using magnetic cell labeling; dextran-coated ultrasmall superparamagnetic iron oxide (USPIOs) nanoparticles with plain surfaces having a hydrodynamic size of 25 nm were used for this study. Tests were carried out in four groups each containing 5 flasks of 5.5 × 10 6 AD-293 embryonic kidney cells. The cell lines were incubated for 24 h using four different iron concentrations with and without protamine sulfate (Pro), washed with phosphate-buffered saline (PBS) and centrifuged three times to remove the unbounded USPIOs. Cell viability was also verified using USPIOs. There...
Deformation prediction of mouse embryos in cell injection experiment by a feedforward artificial neural network
, Article Proceedings of the ASME Design Engineering Technical Conference, 28 August 2011 through 31 August 2011 ; Volume 2, Issue PARTS A AND B , August , 2011 , Pages 543-550 ; 9780791854792 (ISBN) ; Ahmadian, M. T ; Vossoughi, G. R ; Sharif University of Technology
2011
Abstract
In this study, neural network models have been used to predict the mechanical behaviors of mouse embryos. In addition, sensitivity analysis has been carried out to investigate the influence of the significance of input parameters on the mechanical behavior of mouse embryos. In order to reach these purposes two neural network models have been implemented. Experimental data earlier deduced-by [Flückiger, M. (2004). Cell Membrane Mechanical Modeling for Microrobotic Cell Manipulation. Diploma Thesis, ETHZ Swiss Federal Institute of Technology, Zurich, WS03/04]-were collected to obtain training and test data for the neural network. The results of these investigations show that the correlation...