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    An injectable platelet lysate-hyaluronic acid hydrogel supports cellular activities and induces chondrogenesis of encapsulated mesenchymal stem cells

    , Article Acta Biomaterialia ; 2018 ; 17427061 (ISSN) Jooybar, E ; Abdekhodaie, M. J ; Alvi, M ; Mousavi, A ; Karperien, M ; Dijkstra, P. J ; Sharif University of Technology
    Acta Materialia Inc  2018
    Abstract
    Developing scaffolds that can provide cells and biological cues simultaneously in the defect site is of interest in tissue engineering field. In this study, platelet lysate (PL) as an autologous and inexpensive source of growth factors was incorporated into a cell-laden injectable hyaluronic acid-tyramine (HA-TA) hydrogel. Subsequently, the effect of platelet lysate on cell attachment, viability and differentiation of human mesenchymal stem cell (hMSCs) toward chondrocytes was investigated. HA-TA conjugates having a degree of substitution of 20 TA moieties per 100 disaccharide units were prepared and crosslinked in the presence of horseradish peroxidase and low concentrations of hydrogen... 

    Evaluation of differentiation quality of several differentiation inducers of bone marrow-derived mesenchymal stem cells to nerve cells by as-sessing expression of beta-tubulin 3 marker: A systematic review

    , Article Current Stem Cell Research and Therapy ; Volume 16, Issue 8 , 2021 , Pages 994-1004 ; 1574888X (ISSN) Karami Fath, M ; Zahedi, F ; Hashemi, Z. S ; Khalili, S ; Sharif University of Technology
    Bentham Science Publishers  2021
    Abstract
    Neurological diseases have different etiological causes. Contemporary, developing an ef-fective treatment for these diseases is an ongoing challenge. Cell therapy is recognized as one of the promising solutions for the treatment of these diseases. Amongst various types of stem cells, bone marrow-derived mesenchymal stem cells (BM-MSC) are known to be the most widely used stem cells. These cells are endowed with appealing properties such as the ability to differentiate into other cell types, including the muscle, liver, glial, and nerve cells. In this review study, we have systematically evaluated the ability of a variety of chemical compounds used in the last ten years to differentiate... 

    Feasibility Study of Culturing and Differentiating Mesenchymal Stem Cells to Smooth Muscle Cells in Nanostructured PCL Hybrid Scaffold

    , M.Sc. Thesis Sharif University of Technology Moghadasi, Samaneh (Author) ;
    Abstract
    Tissue engineering is an interdisciplinary field that incorporates principles of engineering with the life sciences. Tissue engineering consists of three principle; cells, scaffolds for cells expansion and growth factors. These factors together can help tissue engineers to provide appropriate microenvironments for directing special cells behavior.
    Fibrous scaffolds have found many applications in tissue engineering due to their nanometer dimensions and high surface to volume ratio. Fibrous scaffolds can impart mechanical strength, structure for cell attachment and act as reservoirs for biomolecule delivery in much a same way as the natural fibrous components of the extracellular... 

    Design and Fabrication of Hydrogel Microcarrier for Tissue Engineering

    , M.Sc. Thesis Sharif University of Technology Radaei, Payam (Author) ; Mashayekhan, Shohre (Supervisor) ; Yaghmaei, Soheila (Supervisor)
    Abstract
    Microcarriers (MCs) are an attractive technology with various applications in tissue engineering. In this study, chitosan/gelatin MCs were fabricated with blends of different ratio of chitosan/gelatin, by using a setup containing high voltage electrostatic field and syringe pump. Optimization of blend ratio, voltage and syringe pump flow carried out by the “Design expert” software leads to fabrication of MCs with constant diameter while having various elasticity. Mechanical strength and elasticity of MCs were determined. Human umbilical cord mesenchymal stem cells (hUCMSCs) were cultured on MCs dynamically by using mini-rocher in an incubator. Cells adhesion on MCs were successfully shown by... 

    The synergistic effect of surface topography and sustained release of TGF-β1 on myogenic differentiation of human mesenchymal stem cells

    , Article Journal of Biomedical Materials Research - Part A ; Volume 104, Issue 7 , 2016 , Pages 1610-1621 ; 15493296 (ISSN) Moghadasi Boroujeni, S ; Mashayekhan, S ; Vakilian, S ; Ardeshirylajimi, A ; Soleimani, M ; Sharif University of Technology
    John Wiley and Sons Inc 
    Abstract
    A combination of topographical cues and controlled release of biochemical factors is a potential platform in controlling stem cells differentiation. In this study the synergistic effect of nanotopography and sustained release of biofunctional transforming growth factor beta 1 (TGF-β1) on differentiation of human Wharton's Jelly-derived mesenchymal stem cell (hWJ-derived UC-MSCs) toward myogenic lineage was investigated. In order to achieve a sustained release of TGF-β1, this factor was encapsulated within chitosan nanoparticles. Afterwards the aligned composite mats were fabricated using poly-E-caprolacton (PCL) containing TGF-β1-loaded chitosan nanoparticles and poly-L-lactic acid (PLLA).... 

    Stem Cell Proliferation and Differentiation in Bioreactors

    , M.Sc. Thesis Sharif University of Technology Rezaei, Maryam (Author) ; Vosoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor)
    Abstract
    In this study, differentiation of rabbit embryonic-derived mesenchymal stem cells to osteogenic cells has been characterized. Bone tissue engineering is based upon the understanding of bone tissue construct and it’s formation in-vivo, and the Preparation of tissue engineered bone constructs to repair large size defects is it’s major goal. We sought to investigate the combined effect of three elemnts of tissue engineering: cells, scaffolds and growth factors. Mesenchymal stem cells are unspecialized cells which due to their unlimited self-renewal capacity and the remarkable ability to differentiate along multiple linage pathways are natural choice for application in tissue repair and... 

    Graphene nanogrids for selective and fast osteogenic differentiation of human mesenchymal stem cells

    , Article Carbon ; Volume 59 , 2013 , Pages 200-211 ; 00086223 (ISSN) Akhavan, O ; Ghaderi, E ; Shahsavar, M ; Sharif University of Technology
    2013
    Abstract
    Graphene nanogrids (fabricated by graphene nanoribbons obtained through oxidative unzipping of multi-walled carbon nanotubes) were used as two-dimensional selective templates for accelerated differentiation of human mesenchymal stem cells (hMSCs), isolated from umbilical cord blood, into osteogenic lineage. The biocompatible and hydrophilic graphene nanogrids showed high actin cytoskeleton proliferations coinciding with patterns of the nanogrids. The amounts of proliferations were found slightly better than proliferation on hydrophilic graphene oxide (GO) sheets, and significantly higher than non-uniform proliferations on hydrophobic reduced graphene oxide (rGO) sheets and... 

    Genotoxicity of graphene nanoribbons in human mesenchymal stem cells

    , Article Carbon ; Volume 54 , 2013 , Pages 419-431 ; 00086223 (ISSN) Akhavan, O ; Ghaderi, E ; Emamy, H ; Akhavan, F ; Sharif University of Technology
    2013
    Abstract
    Single-layer reduced graphene oxide nanoribbons (rGONRs) were obtained through an oxidative unzipping of multi-walled carbon nanotubes and a subsequent deoxygenation by hydrazine and bovine serum albumin. Human mesenchymal stem cells (hMSCs) were isolated from umbilical cord blood and used for checking the concentration- and time-dependent cyto- and geno-toxic effects of the rGONRs and reduced graphene oxide sheets (rGOSs). The cell viability assay indicated significant cytotoxic effects of 10 μg/mL rGONRs after 1 h exposure time, while the rGOSs exhibited the same cytotoxicity at concentration of 100 μg/mL after 96 h. The oxidative stress was found as the main mechanism involved in the... 

    Design, fabrication and characterization of oxidized alginate-gelatin hydrogels for muscle tissue engineering applications

    , Article Journal of Biomaterials Applications ; Volume 31, Issue 1 , 2016 , Pages 152-161 ; 08853282 (ISSN) Baniasadi, H ; Mashayekhan, S ; Fadaoddini, S ; Haghirsharifzamini, Y ; Sharif University of Technology
    SAGE Publications Ltd  2016
    Abstract
    In this study, we reported the preparation of self cross-linked oxidized alginate-gelatin hydrogels for muscle tissue engineering. The effect of oxidation degree (OD) and oxidized alginate/gelatin (OA/GEL) weight ratio were examined and the results showed that in the constant OA/GEL weight ratio, both cross-linking density and Young's modulus enhanced by increasing OD due to increment of aldehyde groups. Furthermore, the degradation rate was increased with increasing OD probably due to decrement in alginate molecular weight during oxidation reaction facilitated degradation of alginate chains. MTT cytotoxicity assays performed on Wharton's Jelly-derived umbilical cord mesenchymal stem cells... 

    Enhanced chondrogenic differentiation of human bone marrow mesenchymal stem cells on PCL/PLGA electrospun with different alignments and compositions

    , Article International Journal of Polymeric Materials and Polymeric Biomaterials ; Volume 67, Issue 1 , 2018 , Pages 50-60 ; 00914037 (ISSN) Zamanlui, S ; Mahmoudifard, M ; Soleimani, M ; Bakhshandeh, B ; Vasei, M ; Faghihi, S ; Sharif University of Technology
    Taylor and Francis Inc  2018
    Abstract
    The simultaneous effect of electrospun scaffold alignment and polymer composition on chondrogenic differentiation of human bone marrow mesenchymal stem cells (hBMMSC) is investigated. Aligned and randomly oriented polycaprolactone/poly(lactic-co-glycolic acid) (PLGA) hybrid electrospun scaffolds with two different ratios are fabricated by electrospinning. It is found that aligned nanofibrous scaffolds support higher chondrogenic differentiation of hBMMSCs compared to random ones. The aligned scaffolds show a higher expression level of chondrogenic markers such as type II collagen and aggrecan. It is concluded that the aligned nanofibrous scaffold with higher PLGA ratio could significantly... 

    Magnetoelectric nanocomposite scaffold for high yield differentiation of mesenchymal stem cells to neural-like cells

    , Article Journal of Cellular Physiology ; Volume 234, Issue 8 , 2019 , Pages 13617-13628 ; 00219541 (ISSN) Esmaeili, E ; Soleimani, M ; Ghiass, M. A ; Hatamie, S ; Vakilian, S ; Zomorrod, M. S ; Sadeghzadeh, N ; Vossoughi, M ; Hosseinzadeh, S ; Sharif University of Technology
    Wiley-Liss Inc  2019
    Abstract
    While the differentiation factors have been widely used to differentiate mesenchymal stem cells (MSCs) into various cell types, they can cause harm at the same time. Therefore, it is beneficial to propose methods to differentiate MSCs without factors. Herein, magnetoelectric (ME) nanofibers were synthesized as the scaffold for the growth of MSCs and their differentiation into neural cells without factors. This nanocomposite takes the advantage of the synergies of the magnetostrictive filler, CoFe 2 O 4 nanoparticles (CFO), and piezoelectric polymer, polyvinylidene difluoride (PVDF). Graphene oxide nanosheets were decorated with CFO nanoparticles for a proper dispersion in the polymer through... 

    Study and Fabrication of a Microfluidic System to Investigate Stem Cell Differentiation

    , M.Sc. Thesis Sharif University of Technology Motmaen Esfahani, Shayan (Author) ; Vosoughi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor)
    Abstract
    In recent years, many studies have conducted research about stem cells due to their prominent features such as proliferation and differentiation. By changing the specific parameters of the cellular micro-environment, it is possible to determine the stem cells fate and thereby use them in medical affairs. For this purpose, various devices and methods have been designeds such as microfluidic devices and scaffolds. The microfluidic devices has been especially focuced in the researchs due to their unique features, like the possibility of testing with small amounts of samples, the creation and control of low cost cellular micro-environment and low practical time. Two-dimensional scaffolds made up... 

    Combinatorial screening of nanoclay-reinforced hydrogels: a glimpse of the "holy grail" in orthopedic stem cell therapy?

    , Article ACS Applied Materials and Interfaces ; Volume 10, Issue 41 , 2018 , Pages 34924-34941 ; 19448244 (ISSN) Hasany, M ; Thakur, A ; Taebnia, N ; Kadumudi, F. B ; Shahbazi, M. A ; Pierchala, M. K ; Mohanty, S ; Orive, G ; Andresen, T. L ; Foldager, C. B ; Yaghmaei, S ; Arpanaei, A ; Gaharwar, A. K ; Mehrali, M ; Dolatshahi Pirouz, A ; Sharif University of Technology
    American Chemical Society  2018
    Abstract
    Despite the promise of hydrogel-based stem cell therapies in orthopedics, a significant need still exists for the development of injectable microenvironments capable of utilizing the regenerative potential of donor cells. Indeed, the quest for biomaterials that can direct stem cells into bone without the need of external factors has been the "Holy Grail" in orthopedic stem cell therapy for decades. To address this challenge, we have utilized a combinatorial approach to screen over 63 nanoengineered hydrogels made from alginate, hyaluronic acid, and two-dimensional nanoclays. Out of these combinations, we have identified a biomaterial that can promote osteogenesis in the absence of... 

    Enzymatically crosslinked hyaluronic acid microgels as a vehicle for sustained delivery of cationic proteins

    , Article European Polymer Journal ; Volume 115 , 2019 , Pages 234-243 ; 00143057 (ISSN) Jooybar, E ; Abdekhodaie, M. J ; Mousavi, A ; Zoetebier, B ; Dijkstra, P. J ; Sharif University of Technology
    Elsevier Ltd  2019
    Abstract
    In this study, a novel biodegradable hyaluronic acid (HA) based microgel were prepared via enzymatic crosslinking of tyramine conjugated HA (HA-TA) in an inverse microemulsion. HA-TA microdroplets were crosslinked within a few seconds in the presence of horseradish peroxidase (HRP) and hydrogen peroxide (H 2 O 2 ). The high water content of the polymeric network and the inherent negative charge of the HA-TA microgels provided a suitable platform for encapsulation of cationic proteins like lysozyme and TGF-β1 growth factor. The results demonstrated that lysozyme was released, after an initial burst release, in a suitable sustained manner over a period of four weeks. Both diffusion and... 

    Engineering 3D parallelized microfluidic droplet generators with equal flow profiles by computational fluid dynamics and stereolithographic printing

    , Article Lab on a Chip ; Volume 20, Issue 3 , 2020 , Pages 490-495 Kamperman, T ; Teixeira, L. M ; Salehi, S. S ; Kerckhofs, G ; Guyot, Y ; Geven, M ; Geris, L ; Grijpma, D ; Blanquer, S ; Leijten, J ; Sharif University of Technology
    Royal Society of Chemistry  2020
    Abstract
    Microfluidic droplet generators excel in generating monodisperse micrometer-sized droplets and particles. However, the low throughput of conventional droplet generators hinders their clinical and industrial translation. Current approaches to parallelize microdevices are challenged by the two-dimensional nature of the standard fabrication methods. Here, we report the facile production of three-dimensionally (3D) parallelized microfluidic droplet generators consisting of stacked and radially multiplexed channel designs. Computational fluid dynamics simulations form the design basis for a microflow distributor that ensures similar flow rates through all droplet generators. Stereolithography is... 

    Modeling and optimization of gelatin-chitosan micro-carriers preparation for soft tissue engineering: using response surface methodology

    , Article Materials Science and Engineering C ; Volume 75 , 2017 , Pages 545-553 ; 09284931 (ISSN) Radaei, P ; Mashayekhan, S ; Vakilian, S ; Sharif University of Technology
    Abstract
    Electrospray ionization is a wide spread technique for producing polymeric microcarriers (MCs) by applying electrostatic force and ionic cross-linker, simultaneously. In this study, fabrication process of gelatin-chitosan MCs and its optimization using the Response Surface Methodology (RSM) is reported. Gelatin/chitosan (G/C) blend ratio, applied voltage and feeding flow rate, their individual and interaction effects on the diameter and mechanical strength of the MCs were investigated. The obtained models for diameter and mechanical strength of MCs have a quadratic relationship with G/C blend ratio, applied voltage and feeding flow rate. Using the desirability curve, optimized G/C blend... 

    A dual functional chondro-inductive chitosan thermogel with high shear modulus and sustained drug release for cartilage tissue engineering

    , Article International Journal of Biological Macromolecules ; Volume 205 , 2022 , Pages 638-650 ; 01418130 (ISSN) Dehghan Baniani, D ; Mehrjou, B ; Wang, D ; Bagheri, R ; Solouk, A ; Chu, P. K ; Wu, H ; Sharif University of Technology
    Elsevier B.V  2022
    Abstract
    We report a chitosan-based nanocomposite thermogel with superior shear modulus resembling that of cartilage and dual pro-chondrogenic and anti-inflammatory functions. Two therapeutic agents, kartogenin (KGN) and diclofenac sodium (DS), are employed to promote chondrogenesis of stem cells and suppress inflammation, respectively. To extend the release time in a controlled manner, KGN is encapsulated in the uniform-sized starch microspheres and DS is loaded into the halloysite nanotubes. Both drug carriers are doped into the maleimide-modified chitosan hydrogel to produce a shear modulus of 167 ± 5 kPa that is comparable to that of articular cartilage (50–250 kPa). Owing to the hydrogel... 

    Silica nanoparticle surface chemistry: An important trait affecting cellular biocompatibility in two and three dimensional culture systems

    , Article Colloids and Surfaces B: Biointerfaces ; Volume 182 , 2019 ; 09277765 (ISSN) Hasany, M ; Taebnia, N ; Yaghmaei, S ; Shahbazi, M. A ; Mehrali, M ; Dolatshahi Pirouz, A ; Arpanaei, A ; Sharif University of Technology
    Elsevier B.V  2019
    Abstract
    Great advantages bestowed by mesoporous silica nanoparticles (MSNs) including high surface area, tailorable pore diameter and surface chemistry, and large pore volume render them as efficient tools in biomedical applications. Herein, MSNs with different surface chemistries were synthesized and investigated in terms of biocompatibility and their impact on the morphology of bone marrow-derived mesenchymal stem cells both in 2D and 3D culture systems. Bare MSNs (BMSNs) were synthesized by template removing method using tetraethylorthosilicate (TEOS) as a precursor. The as-prepared BMSNs were then used to prepare amine-functionalized (AMSNs), carboxyl-functionalized (CMSNs) and polymeric... 

    Bioinspired nanofiber scaffold for differentiating bone marrow-derived neural stem cells to oligodendrocyte-like cells: Design, fabrication, and characterization

    , Article International Journal of Nanomedicine ; Volume 15 , 2020 , Pages 3903-3920 Boroojeni, F. R ; Mashayekhan, S ; Abbaszadeh, H. A ; Ansarizadeh, M ; Khoramgah, M. S ; Rahimi Movaghar, V ; Sharif University of Technology
    Dove Medical Press Ltd  2020
    Abstract
    Background: Researchers are trying to study the mechanism of neural stem cells (NSCs) differentiation to oligodendrocyte-like cells (OLCs) as well as to enhance the selective differentiation of NSCs to oligodendrocytes. However, the limitation in nerve tissue acces-sibility to isolate the NSCs as well as their differentiation toward oligodendrocytes is still challenging. Purpose: In the present study, a hybrid polycaprolactone (PCL)-gelatin nanofiber scaffold mimicking the native extracellular matrix and axon morphology to direct the differentiation of bone marrow-derived NSCs to OLCs was introduced. Materials and Methods: In order to achieve a sustained release of T3, this factor was... 

    Study of epithelial differentiation and protein expression of keratinocyte-mesenchyme stem cell co-cultivation on electrospun nylon/B. vulgaris extract composite scaffold

    , Article Materials Science and Engineering C ; Volume 75 , 2017 , Pages 653-662 ; 09284931 (ISSN) Hosseinzadeh, S ; Soleimani, M ; Vossoughi, M ; Ranjbarvan, P ; Hamedi, S ; Zamanlui, S ; Mahmoudifard, M ; Sharif University of Technology
    Abstract
    Employing of the composite electrospun scaffold containing herbal extract in conjugation with co-culturing of cells can open up new window to the design of efficient biomaterials for skin tissue regeneration. Here, we introduce the synergistic effect of composite electrospun nanofibrous scaffold of nylon66 loaded with Beta vulgaris (B. vulgaris) (extract of beet roots, a plants whose widely used in Iranian folk medicine as wound healing medicine) and co-culture of mesenchymal stem-cells (MSCs)-human keratinocyte (H-keratino) differentiation towards epithelial lineage. In vitro biocompatibility was examined through MTT assay and epithelial differentiation checked by real-time PCR and...