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    A rapid and sensitive HPLC-fluorescence method for determination of mirtazapine and its two major metabolites in human plasma

    , Article Iranian Journal of Pharmaceutical Research ; Volume 13, Issue 3 , Summer 2014 , Pages 853-862 Lavasani, H ; Giorgi, M ; Sheikholeslami, B ; Hedayati, M ; Rouini, M. R ; Sharif University of Technology
    A rapid and sensitive HPLC method has been developed for the quantification of mirtazapine (MRZ), a noradrenergic and specific serotonergic inhibitor antidepressant (NaSSA) and its two major metabolites N-desmethyl mirtazapine (NDM) and 8-hydroxymirtazapine (8-OHM) in human plasma. The separation was achieved using Chromolith C18 column and a mobile phase of acetonitrile: phosphate buffer (pH = 3, 20:80, v/v) in isocratic mode at a flow rate of 2 mL/min. A fluorescence detector was set at 290 and 350 nm for excitation and emission, respectively. Zolpidem was used as the internal standard. Liquid-liquid extraction was applied for sample clean up. All analytes were eluted in less than 5... 

    Hydrogen bonding effects on acidity enhancement of barbiturates and their metabolites in gas and solution phase, a DFT study

    , Article Computational and Theoretical Chemistry ; Volume 1196 , 2021 ; 2210271X (ISSN) Daneshfar, M ; Fattahi, A ; Sharif University of Technology
    Elsevier B.V  2021
    Density functional theory method and B3LYP/6-31+G(d) level of theory were used to predict the acidity of barbiturates and their corresponding metabolites in the gas and solution phase (H2O). Polarized continuum model was applied to calculate pKa values of barbiturates and metabolites. A comparison between acidity of barbiturates and metabolites in the gas and solution phase indicates that the acidity strength of barbiturates enhances with the increase of intramolecular hydrogen bonds in metabolites. This acidity can increase to 9.47 kcal/mol in gas phase and 2.73 pKa units in solution phase for a typical metabolite of barbiturate due to the effect of intramolecular hydrogen bonds. Also,... 

    Electrochromism: An emerging and promising approach in (bio)sensing technology

    , Article Materials Today ; Volume 50 , 2021 , Pages 476-498 ; 13697021 (ISSN) Farahmand Nejad, M. A ; Ranjbar, S ; Parolo, C ; Nguyen, E. P ; Álvarez Diduk, R ; Hormozi Nezhad, M. R ; Merkoçi, A ; Sharif University of Technology
    Elsevier B.V  2021
    Electrochromism (EC) is a unique property of certain materials that undergo an electrochemical-induced change in colouration. During the last decades, electrochromic materials (ECMs) have been applied in a variety of technologies ranging from smart windows to information displays and energy storage devices. More recently, ECMs have attracted the attention of the (bio)sensing community thanks to their ability to combine the sensitivity of electrochemical methods with the intuitive readout of optical sensors. Although still a nascent technology, EC-based sensors are on the rise with several targets (e.g. cancer biomarkers, bacteria, metabolites and pesticides), which have already been detected... 

    Disease-related metabolites affect protein-nanoparticle interactions

    , Article Nanoscale ; Volume 10, Issue 15 , 2018 , Pages 7108-7115 ; 20403364 (ISSN) Tavakol, M ; Montazeri, A ; Naghdabadi, R ; Hajipour, M. J ; Zanganeh, S ; Caracciolo, G ; Mahmoudi, M ; Sharif University of Technology
    Royal Society of Chemistry  2018
    Once in biological fluids, the surface of nanoparticles (NPs) is rapidly covered with a layer of biomolecules (i.e., the "protein corona") whose composition strongly determines their biological identity, regulates interactions with biological entities including cells and the immune system, and consequently directs the biological fate and pharmacokinetics of nanoparticles. We recently introduced the concept of a "personalized protein corona" which refers to the formation of different biological identities of the exact same type of NP after being exposed to extract plasmas from individuals who have various types of diseases. As different diseases have distinct metabolomic profiles and... 

    Green synthesis of silica nanoparticles from olive residue and investigation of their anticancer potential

    , Article Nanomedicine ; Volume 16, Issue 18 , 2021 , Pages 1581-1593 ; 17435889 (ISSN) Rezaeian, M ; Afjoul, H ; Shamloo, A ; Maleki, A ; Afjoul, N ; Sharif University of Technology
    Future Medicine Ltd  2021
    Graphical abstract

    The Differential Diagnosis of Crohn's Disease and Celiac Disease Using Nuclear Magnetic Resonance Spectroscopy

    , Article Applied Magnetic Resonance ; Volume 45, Issue 5 , May , 2014 , Pages 451-459 Fathi, F ; Kasmaee, L. M ; Sohrabzadeh, K ; Nejad, M. R ; Tafazzoli, M ; Oskouie, A. A ; Sharif University of Technology
    Crohn's disease and celiac disease belong to a group of autoimmune conditions that affect the digestive system, specifically the small intestine. They both attack the digestive tract and share many symptoms. Thus, the discovery of proper methods would be a major step toward differentiating celiac disease from Crohn's disease. The aim of this study was to search for the metabolic biomarkers to differentiate between these two diseases. Proton nuclear magnetic resonance spectroscopy (1H NMR) was employed as the metabolic profiling method to look for serum metabolites that differentiate between celiac disease and Crohn's disease. Classification of celiac disease and Crohn's disease was done... 

    Relationship between serum level of selenium and metabolites using 1hnmr-based metabonomics in parkinson's disease

    , Article Applied Magnetic Resonance ; Volume 44, Issue 6 , January , 2013 , Pages 721-734 ; 09379347 (ISSN) Fathi, F ; Kyani, A ; Darvizeh, F ; Mehrpour, M ; Tafazzoli, M ; Shahidi, G ; Sharif University of Technology
    Parkinson's disease (PD) is a neurodegenerative disease, which is not easily diagnosed using clinical tests and the discovery of proper methods would be a major step towards a successful diagnosis. In the present study, we employed metabolic profiling using proton nuclear magnetic resonance spectroscopy to find metabolites in serum, which are helpful for the diagnosis of PD. Classification of PD and healthy subject was done using random forest. Serum levels of selenium measured by atomic absorption spectrometry in PD group were lower than the serum selenium levels in the control group. The metabolites causing selenium changes in PD patients were identified using random forest, and a model... 

    Numerical investigations of hepatic spheroids metabolic reactions in a perfusion bioreactor

    , Article Frontiers in Bioengineering and Biotechnology ; Volume 7 , 2019 ; 22964185 (ISSN) Sharifi, F ; Firoozabadi, B ; Firoozbakhsh, K ; Sharif University of Technology
    Frontiers Media S.A  2019
    Miniaturized culture systems of hepatic cells are emerging as a strong tool facilitating studies related to liver diseases and drug discovery. However, the experimental optimization of various parameters involved in the operation of these systems is time-consuming and expensive. Hence, developing numerical tools predicting the function of such systems can significantly reduce the associated cost. In this paper, a perfusion-based three dimensional (3D) bioreactor comprising encapsulated human liver hepatocellular carcinoma (HepG2) spheroids are analyzed. The flow and mass transfer equations for oxygen as well as different metabolites such as albumin, glucose, glutamine, ammonia, and urea were... 

    Biodegradation of polychlorinated biphenyls by lysinibacillus macrolides and bacillus firmus isolated from contaminated soil

    , Article International Journal of Engineering, Transactions B: Applications ; Volume 32, Issue 5 , 2019 , Pages 628-633 ; 1728144X (ISSN) Samadi, A ; Sharifi, H ; Ghobadi Nejad, Z ; Yaghmaei, S ; Sharif University of Technology
    Materials and Energy Research Center  2019
    Polychlorinated biphenyls (PCBs) cause many significant ecological problems because of their low degradability, high harmfulness, and solid bioaccumulation.Two bacterial strains were isolated from soil that had been polluted with electrical transformer liquid for more than 40 years. The isolates were distinguished as Lysinibacillus macrolides DSM54Tand Bacillus firmus NBRC15306Tthrough 16S rRNA sequencing analysis.This is the primary report of an agent of the genus Bacillus firmus equipped for the removal of PCBs. The strains could develop broadly on 2-Chlorobiphenyl and 2,4-dichlorobiphenyl. GC/MS analysis of individual congeners revealed up to 80% degradation of the xenobiotics in 96h,... 

    Analysis of MEOR efficiency to increase recovery in an Iranian reservoir

    , Article Scientia Iranica ; Volume 14, Issue 2 , 2007 , Pages 161-168 ; 10263098 (ISSN) Biria, D ; Roostaazad, R ; Darouneh, E ; Izadi, H ; Sharif University of Technology
    Sharif University of Technology  2007
    Rock samples from the Asmary outcrop formation of the Ahwaz oil rich zone with a porosity of 16% and permeability of 1 md and MIS crude oil with an API value of 42.5 and moderate asphaltene content of 3%, were used to study the effect of the incubation time and flow rate of the displacing fluid in MEOR operations. Five species of rod shaped, gram positive, thermophile and facultative bacteria were isolated and purified from the crude. Due to the high sweep efficiency prevailing in the core flooding system, the effect of the displacing brine flow rate on the oil recovery efficiency was found not to be significant. On the other hand, a 100% increase in incubation time from 7 to 14 days... 

    Advancement of sensor integrated organ-on-chip devices

    , Article Sensors (Switzerland) ; Volume 21, Issue 4 , 2021 , Pages 1-44 ; 14248220 (ISSN) Clarke, G.A ; Hartse, B. X ; Niaraki Asli, A. E ; Taghavimehr, M ; Hashemi, N ; Abbasi Shirsavar, M ; Montazami, R ; Alimoradi, N ; Nasirian, V ; Ouedraogo, L. J ; Hashemi, N. N ; Sharif University of Technology
    MDPI AG  2021
    Organ-on-chip devices have provided the pharmaceutical and tissue engineering worlds much hope since they arrived and began to grow in sophistication. However, limitations for their applicability were soon realized as they lacked real-time monitoring and sensing capabilities. The users of these devices relied solely on endpoint analysis for the results of their tests, which created a chasm in the understanding of life between the lab the natural world. However, this gap is being bridged with sensors that are integrated into organ-on-chip devices. This review goes in-depth on different sensing methods, giving examples for various research on mechanical, electrical resistance, and bead-based... 

    Evaluation of aspergillus niger and penicillium simplicissimum for their ability to leach Zn–Ni–Cu from waste mobile phone printed circuit boards

    , Article Journal of Material Cycles and Waste Management ; 2021 ; 14384957 (ISSN) Arshadi, M ; Esmaeili, A. R ; Yaghmaei, S ; Arab, B ; Sharif University of Technology
    Springer Japan  2021
    In this research, Zn, Ni, and Cu recovery from mobile phone printed circuit boards was investigated. The initial pH and pulp density using Aspergillus niger or Penicillium simplicissimum fungi were optimized to improve the recovery of Zn, Ni, and Cu using a central composite design. Fungi were able to recover 97% of Cu. Often for Ni recovery, A. niger was more effective, but in low pulp densities and low pH, P. simplicissimum was preferred. For recovery of Zn, A. niger is more appropriate at pH lower than 6, but P. simplicissimum outperforms at pH higher than 6. Under the optimum conditions (pulp density of 4 gL−1 and initial pH 10), the respective recovery of Cu, Ni, and Zn was determined... 

    Chemometric techniques coupled with NMR for matabolic profiling of lettuce exposed to polycyclic aromatic hydrocarbones

    , Article Analytical Biochemistry ; Volume 611 , 2020 Feizi, N ; Seraj, M ; Tajali, R ; Shavandi, S. R ; Parastar, H ; Sharif University of Technology
    Academic Press Inc  2020
    Treated waste water (TWW) quality varies due to the occurrence of polycyclic aromatic hydrocarbons (PAHs) up to low μg L−1. In this study, a non-targeted metabolomic analysis was performed on lettuce (Lactuca sativa L) exposed to 4 PAHs by irrigation. The plants were watered with different concentrations of contaminants (0–100 μg L−1) for 39 days under controlled conditions and then harvested, extracted and analyzed by nuclear magnetic resonance (NMR). Different chemometric tools based on principal component analysis (PCA) and partial least square discriminant analysis (PLS-DA) are proposed for the analysis of the complex data sets generated in the different exposure experiments.... 

    Naphthalene metabolism in Nocardia otitidiscaviarum strain TSH1, a moderately thermophilic microorganism

    , Article Chemosphere ; Volume 72, Issue 6 , 2008 , Pages 905-909 ; 00456535 (ISSN) Zeinali, M ; Vossoughi, M ; Ardestani, S. K ; Sharif University of Technology
    The thermophilic bacterium Nocardia otitidiscaviarum strain TSH1, originally isolated in our laboratory from a petroindustrial wastewater contaminated soil in Iran, grows at 50 °C on a broad range of hydrocarbons. Transformation of naphthalene by strain TSH1 which is able to use this two ring-polycyclic aromatic hydrocarbon (PAH) as a sole source of carbon and energy was investigated. The metabolic pathway was elucidated by identifying metabolites, biotransformation studies and monitoring enzyme activities in cell-free extracts. The identification of metabolites suggests that strain TSH1 initiates its attack on naphthalene by dioxygenation at its C-1 and C-2 positions to give... 

    Quantitative in vivo microsampling for pharmacokinetic studies based on an integrated solid-phase microextraction system

    , Article Analytical Chemistry ; Volume 79, Issue 12 , 2007 , Pages 4507-4513 ; 00032700 (ISSN) Zhang, X ; Eshaghi, A ; Musteata, F. M ; Ouyang, G ; Pawliszyn, J ; Sharif University of Technology
    An integrated microsampling approach based on solid-phase microextraction (SPME) was developed to provide a complete solution to highly efficient and accurate pharmacokinetic studies. The microsampling system included SPME probes that are made of poly(ethylene glycol) (PEG) and C18-bonded silica, a fast and efficient sampling strategy with accurate kinetic calibration, and a high-throughput desorption device based on a modified 96-well plate. The sampling system greatly improved the quantitative capability of SPME in two ways. First, the use of the C18-bonded silica/PEG fibers minimized the competition effect from analogues of the target analytes in a complicated sample matrix such as blood... 

    Chromatographic fingerprint analysis of secondary metabolites in citrus fruits peels using gas chromatography-mass spectrometry combined with advanced chemometric methods

    , Article Journal of Chromatography A ; Volume 1251 , 2012 , Pages 176-187 ; 00219673 (ISSN) Parastar, H ; Jalali Heravi, M ; Sereshti, H ; Mani Varnosfaderani, A ; Sharif University of Technology
    Multivariate curve resolution (MCR) and multivariate clustering methods along with other chemometric methods are proposed to improve the analysis of gas chromatography-mass spectrometry (GC-MS) fingerprints of secondary metabolites in citrus fruits peels. In this way, chromatographic problems such as baseline/background contribution, low S/N peaks, asymmetric peaks, retention time shifts, and co-elution (overlapped and embedded peaks) occurred during GC-MS analysis of chromatographic fingerprints are solved using the proposed strategy. In this study, first, informative GC-MS fingerprints of citrus secondary metabolites are generated and then, whole data sets are segmented to some... 

    Combining multivariate image analysis with high-performance thin-layer chromatography for development of a reliable tool for saffron authentication and adulteration detection

    , Article Journal of Chromatography A ; Volume 1628 , 2020 Amirvaresi, A ; Rashidi, M ; Kamyar, M ; Amirahmadi, M ; Daraei, B ; Parastar, H ; Sharif University of Technology
    Elsevier B.V  2020
    In this work, high-performance thin-layer chromatography (HPTLC) coupled with multivariate image analysis (MIA) is proposed as a fast and reliable tool for authentication and adulteration detection of Iranian saffron samples based on their HPTLC fingerprints. At first, the secondary metabolites of saffron were extracted using ultrasonic-assisted solvent extraction (UASE) which was optimized using central composite design (CCD). Next, the RGB coordinates of HPTLC images were used for estimation of saffron origin based on principal component analysis (PCA). The PCA scores plot showed that saffron samples were clustered into two clear-cut groups which was 92% matched with the geographical... 

    Investigation of wettability alteration through relative permeability measurement during MEOR process: A micromodel study

    , Article Journal of Petroleum Science and Engineering ; Vol. 120, issue , 2014 , p. 10-17 Khajepour, H ; Mahmoodi, M ; Biria, D ; Ayatollahi, S ; Sharif University of Technology
    Microbial Enhanced Oil Recovery (MEOR) as a tertiary process employs microorganisms and their metabolites to reduce the residual oil saturation of the reservoir mainly through interfacial tension (IFT) reduction and wettability alteration. In spite of its great potential and the mentioned advantages, application of MEOR has been limited because of the lack of practical convincing experimental results. In this study, the effects of MEOR process on wettability changes and the reduction of residual oil saturation have been examined by providing microscopic visualization of two phase flow in transparent glass micromodels. Biosurfactant producing bacterial strain (Enterobacter cloacae) was... 

    Metabonomics based NMR in Crohn's disease applying PLS-DA

    , Article Gastroenterology and Hepatology from Bed to Bench ; Volume 6, Issue SUPPL , 2013 , Pages S82-S86 ; 20082258 (ISSN) Fathi, F ; Oskouie, A. A ; Tafazzoli, M ; Naderi, N ; Sohrabzedeh, K ; Fathi, S ; Norouzinia, M ; Nejad, M. R ; Sharif University of Technology
    Aim: The aim of this study was to search for metabolic biomarkers of Crohn's disease (CD). Background: Crohn's disease (CD) is a type of inflammatory bowel disease that causes a wide variety of symptoms. CD can influence any part of the gastrointestinal tract from mouth to anus. CD is not easily diagnosed because monitoring tools are currently insufficient. Thus, the discovery of proper methods is needed for early diagnosis of CD. Patients and methods: We utilized metabolic profiling using proton nuclear magnetic resonance spectroscopy (1HNMR) to find the metabolites in serum. Classification of CD and healthy subject was done using partial least squares discriminant analysis (PLS-DA).... 

    NMR based metabonomics study on celiac disease in the blood serum

    , Article Gastroenterology and Hepatology from Bed to Bench ; Volume 6, Issue 4 , 2013 , Pages 190-194 ; 20082258 (ISSN) Fathi, F ; Ektefa, F ; Arefi Oskouie, A ; Rostami, K ; Rezaei Tavirani, M ; Mohammad Alizadeh, A. H ; Tafazzoli, M ; Rostami Nejad, M ; Sharif University of Technology
    Aim: The aim of this study is to look for the proper methods that would be a major step towards untreated CD diagnosis and seek the metabolic biomarkers causes of CD and compare them to control group. Background: Celiac disease (CD) is a common autoimmune disorder that is not easily diagnosed using the clinical tests. Patients and methods: Thirty cases and 30 controls were entered into this study. Metabolic profiling was obtained using proton nuclear magnetic resonance spectroscopy (1HNMR) to seek metabolites that are helpful for the detection of CD. Classification of CD and healthy subject was done using random forest (RF). Results: The obtained classification model showed an 89% correct...