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    Municipal wastewater treatment by semi-continuous and membrane algal-bacterial photo-bioreactors

    , Article Journal of Water Process Engineering ; Volume 36 , 2020 Amini, E ; Babaei, A ; Mehrnia, M. R ; Shayegan, J ; Safdari, M. S ; Sharif University of Technology
    Elsevier Ltd  2020
    Abstract
    Conventional wastewater treatment systems demand a high cost mechanical aeration. Using algal-bacterial systems helps to reach a cost-efficient treatment method by eliminating mechanical aeration, since algae produce the oxygen needed for treatment process. In this study, two groups of experiments were performed for domestic wastewater treatment processes. For the first group, semi-continuous microalgae-bacterial photo-bioreactors were cultivated. Chlorella Vulgaris and activated sludge (AS) were used as microalgae and bacterial inoculums, respectively. The effect of different algae and AS ratios on the chemical oxygen demand (COD), N-NH4 + and P-PO4 3− removal was studied. The removal... 

    Choosing a Suitable Microalgae for Bioremediation of Heavy Metals Regarding Iran's Clamate

    , M.Sc. Thesis Sharif University of Technology Alizadeh, Mahsa (Author) ; Yaghmaei, Soheyla (Supervisor)
    Abstract
    The pollution of water resources with heavy metals as a result of industrial activities threatens the lives of all living organisms. Therefore, separation of heavy metals from wastewater before entering our invironment is very important. Various chemical and physical methods such as absorption, chemical precipitation, extraction by solvent and ion exchange can be used to separate heavy metals but each of these methods have their own problems such as imperfect separation of heavy metals, need for expensive facilities and refining systems, high energy consumption and leaving pollutant residuals that need to be separated. On the other hand, the methods mentioned above can not separate heavy... 

    Experimental Studies on Recovery Process of Microalgae using Electric Current and Membrane

    , M.Sc. Thesis Sharif University of Technology Sabermanesh, Mohammad Hossein (Author) ; Vosughi, Manoochehr (Supervisor) ; Moslemi, Mohammad Reza (Supervisor)
    Abstract
    Microalgae is a major problem in water resources due to its adverse influence on water treatment process and water quality like color, odor and taste. In addition microalgae removal is an important process in food and pharmacy industry. Electrocoagulation is a potential method for microalgae removal. Another method for microalgae recovery is membrane treatment. This study is focusing on using both methods separately and simultaneously. To achieve this, the removal of Chlorella Vulgaris, a fresh water microalgae, was used in experiments. In first phase the experiments were solely on electrocoagulation and the effect of several parameters including current density, electrocoagulation duration,... 

    Municipal Wastewater Treatment Using Microalgal-Sludge System

    , M.Sc. Thesis Sharif University of Technology Amini, Elham (Author) ; Shaygan Salek, Jalaloddin (Supervisor) ; Mehrnia, Mohammad Reza (Supervisor)
    Abstract
    If wastewater is not properly treated, then the environment and human health can be negatively impacted. These impacts can include harm to fish and wildlife populations, oxygen depletion and contamination of drinking water. In the typical wastewater treatment process, the goal of primary, secondary and tertiary effluent treatment is to reduce or remove organic matter, solids, nutrients, disease-causing organisms and other pollutants from the treated wastewater before it is discharged to a body of water. Biological methods for wastewater treatment are much preferable for being more economical and environmentally friendly. Biological treatment of wastewater using algal-bacterial culture... 

    Experimental Studies and Optimization of Microalgae Cultivation in a Photo-Bioreactor for Bio-Diesel Production

    , M.Sc. Thesis Sharif University of Technology Abdollazadeh, Himan (Author) ; Vossoghi, Manouchehr (Supervisor) ; Alemzadeh, Iran (Supervisor)

    Experimental and Theoretical Study of the Transesterification Products Extraction in Biodiesel Production

    , M.Sc. Thesis Sharif University of Technology Hakim, Maziar (Author) ; Vossoughi, Manoochehr (Supervisor) ; Rashtchian, Davood (Supervisor)
    Abstract
    The experimental investigation of biodiesel production stages from microalgae especially biomass production stage was conducted in this thesis. The optimal rate of biomass productivity was determined.
    A photobioreactor was designed and constructed to cultivate microalgae C. Vulgaris according to researchers’ works and obtained results in this study. The process parameters were examined and the optimal parameters were obtained according to the necessary tests which were carried out.
    According to the results, the optimal condition for growing of microalgae was obtained with aeration of 6 lit/min with 3% CO2, and CO2 injection regime of 45 sec. injection and 3 min. of stoppage. A rapid... 

    Experimental Study and Process Design of Oil Extraction from Microalgae Chlorella Vulgaris to Produce Biodiesel

    , M.Sc. Thesis Sharif University of Technology Hassani, Iman (Author) ; Farhadi, Fathollah (Supervisor) ; Vossoughi, Manoochehr (Supervisor)
    Abstract
    The experimental investigation of biodiesel production stages from microalgae especially lipid extraction stage was conducted in this thesis. The optimal rate of biomass productivity and optimal method in lipid extraction for biodiesel production was determined.
    A photobioreactor was designed and constructed to cultivate microalgae C. Vulgaris according to researchers’ works and obtained results in this study. The process parameters were examined and the optimal parameters were obtained according to the necessary tests which were carried out. Then, several methods of lipid extraction using different solvents such as chloroform, methanol, and n-hexane were investigated and the best... 

    Effect of culture age and initial inoculum size on lipid accumulation and productivity in a hybrid cultivation system of Chlorella vulgaris

    , Article Process Safety and Environmental Protection ; Volume 104 , 2016 , Pages 111-122 ; 09575820 (ISSN) Heidari, M ; Kariminia, H. R ; Shayegan, J ; Sharif University of Technology
    Institution of Chemical Engineers 
    Abstract
    Chlorella vulgaris was cultivated in a hybrid (two-stage) system. The effect of the transferring time from nutrient-replete phase with a low light intensity (photobioreactor) to the nutrient deprivation phase (open pond) with a higher light intensity, as well as the effect of initial cell concentration in the deprivation phase, on the growth rate and lipid content of the microalgae was investigated. The microalgae were transferred to the nutrient deprived medium at different intervals with various initial cell concentrations. Transferring the cultivated medium of the 4th day with the initial cell concentration of 66 mg L−1 into the deprivation phase resulted in a highest lipid productivity... 

    Evaluation of nutrient removal and biomass production through mixotrophic, heterotrophic, and photoautotrophic cultivation of chlorella in nitrate and ammonium wastewater

    , Article International Journal of Environmental Research ; Volume 12, Issue 2 , 2018 , Pages 167-178 ; 17356865 (ISSN) Babaei, A ; Mehrnia, M. R ; Shayegan, J ; Sarrafzadeh, M. H ; Amini, E ; Sharif University of Technology
    Springer International Publishing  2018
    Abstract
    In this study, the effect of mixotrophic, heterotrophic, photoautotrophic (CO2 of air source), and photoautotrophic (bicarbonate source) cultivation of Chlorella vulgaris was investigated on microalgae growth rate and nutrient removal under different kinds of nitrogen sources. The highest N–NH3 +, N–NO3 −, P–PO4 3− (in ammonium source), and P–PO4 3− (in nitrate source) removal efficiency (87.28 ± 0.89, 70.00 ± 1.20, 87.00 ± 3.10, and 78.10 ± 1.95%, respectively) was reached in the mixotrophic culture. In all cultivations, when nitrate was used as the nitrogen source, specific growth rate, biomass productivity, and specific oxygen production rate (SOPR) were higher than the one with ammonium... 

    Experimental and Theoretical Study of the Lipid Extraction from Wet Microalgae for Biodiesel Production

    , M.Sc. Thesis Sharif University of Technology Malekzadeh, Mohammad (Author) ; Rashtchian, Davood (Supervisor) ; Vossoughi, Manoochehr (Supervisor)
    Abstract
    In this study, different stages of biodiesel production from microalgae were investigated, specifically the extraction step.Various cultivation methods were also studied among which cultivation under stress was the best. Different carbon sources were also investigated to achieve highest growth. Sodium acetate with complete growth was achieved after 8 days with 6.22% fatty acid recovery. Different mixtures of solvents were compared with Blye and Dier method. Results showed that the mixture of hexane and methanol with crude lipid extraction of 15% and fatty acid recovery of 3.97% were chosen as the solvent mixture of equilibria experiments.Organic solvent composed of hexane (non-polar... 

    Experimental Study and Modeling of Lipid Extraction in Downstream Processing of Biodiesel Production from Microalgae

    , Ph.D. Dissertation Sharif University of Technology Abedini Najafabadi, Hamed (Author) ; Vossoughi, Manouchehr (Supervisor) ; Pazuki, Gholamreza (Supervisor)
    Abstract
    In this research, experimental study and modeling of biodiesel production process from microalgae has been investigated. First, to have a biomass with suitable lipid content, a novel two-step process based on cultivation under nutrient sufficient followed by cultivation under nitrogen starvation condition has been proposed. Then the effect of using different organic and inorganic carbon sources on this process has been investigated which the results indicated that sodium acetate had a better performance compare to other carbon sources. In the following, the effect of using different light regimes on growth rate, lipid content and fatty acid composition have been investigated. Experimental... 

    Experimental and Theoretical Study of Biomass Conversion to Green Fuel by Using Supercritical Fluid

    , M.Sc. Thesis Sharif University of Technology Mohamadzadeh Shirazi, Hamed (Author) ; Ghotbi, Cyrus (Supervisor) ; Karimi Sabet, Javad (Supervisor)
    Abstract
    Nowadays, one of the most basic human problem is how to manage energy resources for sustainable development and how to deal with problems caused by growing energy consumption. Various scenarios are being discussd that the most important of them can be considered research on finding alternative energy sources that are renewable and do not have common pollution. Thus, according to researches carried out until now, using sources such as wind, water, solar biomass, etc. have been proposed. However, due to problems in the development of each of these sources, biomass as the primary option has been introduced. because biomass has several subsidiaries, research shows microalgaes (as a subset) are... 

    Comparison of different trophic cultivations in microalgal membrane bioreactor containing N-riched wastewater for simultaneous nutrient removal and biomass production

    , Article Process Biochemistry ; Volume 51, Issue 10 , 2016 , Pages 1568-1575 ; 13595113 (ISSN) Babaei, A ; Mehrnia, M. R ; Shayegan, J ; Sarrafzadeh, M. H ; Sharif University of Technology
    Elsevier Ltd  2016
    Abstract
    In this study, a submerged membrane was installed in a bioreactor to treat N-riched wastewater and obtain high biomass productivity. Chlorella vulgaris was cultivated under mixotrophic, heterotrophic, and photoautotrophic conditions in different kinds of nitrogen sources (nitrate and ammonium) to compare the microalgae growth and nutrient removal in a membrane bioreactor. Further, the respirometric and photosynthetic activities of microalgae were investigated to evaluate the viability of microalgae in different conditions. The highest biomass productivity was obtained under mixotrophic cultivation in ammonium source (0.230 ± 0.009 gr/L d). Moreover, with this type of cultivation and nitrogen... 

    The effect of different light intensities and light/dark regimes on the performance of photosynthetic microalgae microbial fuel cell

    , Article Bioresource Technology ; Volume 261 , 2018 , Pages 350-360 ; 09608524 (ISSN) Bazdar, E ; Roshandel, R ; Yaghmaei, S ; Mardanpour, M. M ; Sharif University of Technology
    Elsevier Ltd  2018
    Abstract
    This study develops a photosynthetic microalgae microbial fuel cell (PMMFC) engaged Chlorella vulgaris microalgae to investigate effect of light intensities and illumination regimes on simultaneous production of bioelectricity, biomass and wastewater treatment. The performance of the system under different light intensity (3500, 5000, 7000 and 10,000 lx) and light/dark regimes (24/00, 12/12, 16/8 h) was investigated. The optimum light intensity and light/dark regimes for achieving maximum yield of PMMFC were obtained. The maximum power density of 126 mW m−3, the coulombic efficiency of 78% and COD removal of 5.47% were achieved. The maximum biomass concentration of 4 g l−1 (or biomass yield... 

    Experimental Study of Water Desalination and Electricity Generation by Algae

    , M.Sc. Thesis Sharif University of Technology Khazraee Zamanpour, Morvarid (Author) ; Kariminia, Hamid Reza (Supervisor) ; Vosoughi, Manouchehr (Supervisor)
    Abstract
    Microbial Fuel cells (MFCs) can be used to generate bioenergy in the form of electricity by using microorganisms and natural biological processes. The need for a supply of fresh water in many parts of the world has led to the innovation of modifying an MFC into a Microbial Desalination Cell (MDC) that adds the function of desalination to the MFCs existing ability to generate electricity directly from wastewater. The goals of this project were to design and analyze the use of a Microbial Desalination Cell (MDC) for the generation of electricity while simultaneously desalinating saline water and also to evaluate performance of bio-cathode in MFCs and MDCs. The newly designed MDC incorporates... 

    Improvement of size-based particle separation throughput in slanted spiral microchannel by modifying outlet geometry

    , Article Electrophoresis ; Volume 41, Issue 5-6 , February , 2020 , Pages 353-359 Mihandoust, A ; Maleki Jirsaraei, N ; Rouhani, S ; Safi, S ; Alizadeh, M ; Sharif University of Technology
    Wiley-VCH Verlag  2020
    Abstract
    The inertial microfluidic technique, as a powerful new tool for accurate cell/particle separation based on the hydrodynamic phenomenon, has drawn considerable interest in recent years. Despite numerous microfluidic techniques of particle separation, there are few articles in the literature on separation techniques addressing external outlet geometry to increase the throughput efficiency and purity. In this work, we report on a spiral inertial microfluidic device with high efficiency (>98%). Herein, we demonstrate how changing the outlet geometry can improve the particle separation throughput. We present a complete separation of 4 and 6 μm from 10 μm particles potentially applicable to... 

    Light harvesting and photocurrent generation by nanostructured photoelectrodes sensitized with a photosynthetic pigment: A new application for microalgae

    , Article Bioresource Technology ; Volume 163 , July , 2014 , Pages 1-5 ; ISSN: 09608524 Mohammadpour, R ; Janfaza, S ; Abbaspour Aghdam, F ; Sharif University of Technology
    Abstract
    Here in this study, successful conversion of visible light into electricity has been achieved through utilizing microalgal pigments as a sensitizer of nanostructured photo-electrode of dye-sensitized solar cells (DSSCs). For the first time, photosynthetic pigments extracted from microalgae grown in wastewater is employed to imitate photosynthesis process in bio-molecule-sensitized solar cells. Two designs of photoanode were employed: 10μm nanoparticular TiO2 electrode and 20μm long self-ordered, vertically oriented nanotube arrays of titanium dioxide films. Microalgal photosynthetic pigments are loaded on nanostructured electrodes and their photovoltaic performances have been investigated.... 

    Biodiesel production from Spirulina microalgae feedstock using direct transesterification near supercritical methanol condition

    , Article Bioresource Technology ; Volume 239 , 2017 , Pages 378-386 ; 09608524 (ISSN) Mohamadzadeh Shirazi, H ; Karimi Sabet, J ; Ghotbi, C ; Sharif University of Technology
    Elsevier Ltd  2017
    Abstract
    Microalgae as a candidate for production of biodiesel, possesses a hard cell wall that prevents intracellular lipids leaving out from the cells. Direct or in situ supercritical transesterification has the potential for destruction of microalgae hard cell wall and conversion of extracted lipids to biodiesel that consequently reduces the total energy consumption. Response surface methodology combined with central composite design was applied to investigate process parameters including: Temperature, Time, Methanol-to-dry algae, Hexane-to-dry algae, and Moisture content. Thirty-two experiments were designed and performed in a batch reactor, and biodiesel efficiency between 0.44% and 99.32% was... 

    Lipid production in mixotrophic cultivation of Chlorella vulgaris in a mixture of primary and secondary municipal wastewater

    , Article Renewable Energy ; Vol. 71 , November , 2014 , pp. 502-508 ; ISSN: 09601481 Ebrahimian, A ; Kariminia, H. R ; Vosoughi, M ; Sharif University of Technology
    Abstract
    Microalgae's biomass productivity and oil content depend heavily on the method of its cultivation. In this study, nutrient removal from municipal wastewater by Chlorella vulgaris in batch culture was investigated. Carbon dioxide was supplied from sodium hydrogen carbonate. Effect of parameters including light intensity, sodium hydrogen carbonate concentration, and daily illumination time on the productivity of biomass and lipid was investigated. Lipid and biomass production of C.vulgaris increased at higher concentration of sodium hydrogen carbonate concentration and higher light intensity until a certain value and then decreased, but longer daily illumination time, increased both biomass... 

    Experimental study and thermodynamic modeling for determining the effect of non-polar solvent (hexane)/polar solvent (methanol) ratio and moisture content on the lipid extraction efficiency from Chlorella vulgaris

    , Article Bioresource Technology ; Volume 201 , 2016 , Pages 304-311 ; 09608524 (ISSN) Malekzadeh, M ; Abedini Najafabadi, H ; Hakim, M ; Feilizadeh, M ; Vossoughi, M ; Rashtchian, D ; Sharif University of Technology
    Elsevier Ltd 
    Abstract
    In this research, organic solvent composed of hexane and methanol was used for lipid extraction from dry and wet biomass of Chlorella vulgaris. The results indicated that lipid and fatty acid extraction yield was decreased by increasing the moisture content of biomass. However, the maximum extraction efficiency was attained by applying equivolume mixture of hexane and methanol for both dry and wet biomass. Thermodynamic modeling was employed to estimate the effect of hexane/methanol ratio and moisture content on fatty acid extraction yield. Hansen solubility parameter was used in adjusting the interaction parameters of the model, which led to decrease the number of tuning parameters from 6...