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    Isolation and screening of Bacillus subtilis MJ01 for MEOR application: biosurfactant characterization, production optimization and wetting effect on carbonate surfaces

    , Article Journal of Petroleum Exploration and Production Technology ; Volume 9, Issue 1 , 2019 , Pages 233-245 ; 21900558 (ISSN) Jahanbani Veshareh, M ; Ganji Azad, E ; Deihimi, T ; Niazi, A ; Ayatollahi, S ; Sharif University of Technology
    Springer Verlag  2019
    The bacterial strain MJ01 was isolated from stock tank water of one of the Iranian south oil field production facilities. The 16S rRNA gene of isolate, MJ01, showed 99% similarity to Bacillus subtilis. The results revealed that biosurfactant produced by this strain was lipopeptide-like surfactin based on FTIR analysis. Critical micelle concentration of produced surfactin in distilled water was 0.06 g/l. Wettability study showed that at zero salinity surfactin can change original oil-wet state to water-wet state, but in seawater salinity it cannot modify the wettability significantly. To utilize this biosurfactant in ex situ MEOR process, economical and reservoir engineering technical... 

    Investigating Wettability Alteration During MEOR Process

    , M.Sc. Thesis Sharif University of Technology Soleymani, Fatemeh (Author) ; Ayatollahi, Shahab (Supervisor) ; Kariminia, Hamid Reza (Supervisor)
    Microbial enhanced oil recovery (MEOR) is a collection of techniques that utilize microorganisms and their metabolic products to improve the recovery of crude oil from reservoir rock. From the advantages of this method, independency to the oil price and environmental benign can be mentioned. Many mechanisms have been proposed to contribute on MEOR, but still there is no enough knowledge about the mechanisms that causes inabilities in manipulating the process to get more oil production.
    In the present study, the effect of a native type of bacteria, Enterobacter cloacae, on the glass surfaces wettability which were aged in expose of model oil with different asphaltene concentration was... 

    Bacteria cell hydrophobicity and interfacial properties relationships: A new MEOR approach

    , Article Colloids and Interfaces ; Volume 5, Issue 4 , 2021 ; 25045377 (ISSN) Ganji Azad, E ; Javadi, A ; Jahanbani Veshareh, M ; Ayatollahi, S ; Miller, R ; Sharif University of Technology
    MDPI  2021
    For microbial enhanced oil recovery (MEOR), different mechanisms have been introduced. In some of these papers, the phenomena and mechanisms related to biosurfactants produced by certain microorganisms were discussed, while others studied the direct impacts of the properties of microorganisms on the related mechanisms. However, there are only very few papers dealing with the direct impacts of microorganisms on interfacial properties. In the present work, the interfacial properties of three bacteria MJ02 (Bacillus Subtilis type), MJ03 (Pseudomonas Aeruginosa type), and RAG1 (Acinetobacter Calcoaceticus type) with the hydrophobicity factors 2, 34, and 79% were studied, along with their direct... 

    Microorganisms’ effect on the wettability of carbonate oil-wet surfaces: implications for MEOR, smart water injection and reservoir souring mitigation strategies

    , Article Journal of Petroleum Exploration and Production Technology ; Volume 10, Issue 4 , 2020 , Pages 1539-1550 Jahanbani Veshareh, M ; Ayatollahi, S ; Sharif University of Technology
    Springer  2020
    In upstream oil industry, microorganisms arise some opportunities and challenges. They can increase oil recovery through microbial enhanced oil recovery (MEOR) mechanisms, or they can increase production costs and risks through reservoir souring process due to H2S gas production. MEOR is mostly known by bioproducts such as biosurfactant or processes such as bioclogging or biodegradation. On the other hand, when it comes to treatment of reservoir souring, the only objective is to inhibit reservoir souring. These perceptions are mainly because decision makers are not aware of the effect microorganisms’ cell can individually have on the wettability. In this work, we study the individual effect... 

    Growth kinetic Analysis of Biosurfactant Producing Bacteria for Oil Reservoir Enhance Oil Recovery

    , M.Sc. Thesis Sharif University of Technology Maghsoudi, Ehsan (Author) ; Roosta Azad, Reza (Supervisor) ; Yaghmaei, Soheyla (Supervisor)
    Evidently, environment contamination by water insoluble chemicals present at the industrial wastes could bring about catastrophic consequences to the biosphere. Biological based cleaning technologies are commonly considered as a practical remedy to remove these contaminations. In fact, these techniques are founded on the ability of certain microorganisms to degrade the contaminants. It is crystal clear that the availability of contaminants to the microorganisms is an essential parameter determining the final efficiency of the biodegradation process. In practice, the limited solubility of the contaminants in the aqueous phase leads to mass transfer constraints which consequently, decrease the... 

    Microorganisms Effects on Interfacial Tension and Wettability During Smart Water Injection in Oil Reservoirs

    , M.Sc. Thesis Sharif University of Technology Jahanbani Veshareh, Moein (Author) ; Ayatollahi, Shahabodin (Supervisor) ; Massihi, Mohsen (Supervisor)
    This research was done for feasibility evaluation and understanding correct microbial enhanced oil recovery(MEOR) procedure for carbonate reservoirs. With the aim of cost reduction and possible activation of smart water mechanisms, sea water used as a base for MEOR process. Two biosurfactant producing strains were isolated from oil contaminated water and soil, and one halotolerant strain, from sea water. Optimization of biosurfactant yield was conducted and two new definitions for optimum point, by considering economical and technical facts, were proposed. Partial characterization of produced biosurfactant was carried out through FTIR and NMR analysis and the produced biosurfactant was... 

    Laboratory studies of MEOR in micromodel as a fractured system

    , Article Society of Petroleum Engineers Eastern Regional Meeting 2007: Unbridling Energy's Future, Lexington, KY, 17 October 2007 through 18 October 2007 ; October , 2007 , Pages 39-46 ; 9781604239362 (ISBN) Nourani, M ; Panahi, H ; Biria, D ; Roosta, R ; Haghighi, M ; Mohebbi, A ; Sharif University of Technology
    Microbial EOR (MEOR) is receiving renewed interest worldwide. The method is based on microorganisms' activities to reduce residual oil of reservoirs, which is dependent on behavior of inherent microorganisms or injection of bioproduct of external microorganisms. Five bacterial species were taken from MIS crude oil that is one of the aging Persian fractured reservoirs. Visualization experiments were carried out to examine the behavior of MEOR in micromodels designed to resemble the fractured system: static and dynamic. A new surface sample of the MIS oil was taken at the well head and physical specifications were measured at the lab. This was fairly light oil with API gravity of 42.5. The... 

    The simulation of microbial enhanced oil recovery by using a two-layer perceptron neural network

    , Article Petroleum Science and Technology ; Vol. 32, Issue. 22 , 2014 , pp. 2700-2707 ; ISSN: 10916466 Morshedi, S ; Torkaman, M ; Sedaghat, M. H ; Ghazanfari M.H ; Sharif University of Technology
    The authors simulated a reservoir by using two-layer perceptron. Indeed a model was developed to simulate the increase in oil recovery caused by bacteria injection into an oil reservoir. This model was affected by reservoir temperature and amount of water injected into the reservoir for enhancing oil recovery. Comparing experimental and simulation results and also the erratic trend of data show that the neural networks have modeled this system properly. Considering the effects of nonlinear factors and their erratic and unknown impacts on recovered oil, the perceptron neural network can develop a proper model for oil recovery factor in various conditions. The neural networks have not been... 

    Investigation of wettability alteration through relative permeability measurement during MEOR process: A micromodel study

    , Article Journal of Petroleum Science and Engineering ; Vol. 120, issue , 2014 , p. 10-17 Khajepour, H ; Mahmoodi, M ; Biria, D ; Ayatollahi, S ; Sharif University of Technology
    Microbial Enhanced Oil Recovery (MEOR) as a tertiary process employs microorganisms and their metabolites to reduce the residual oil saturation of the reservoir mainly through interfacial tension (IFT) reduction and wettability alteration. In spite of its great potential and the mentioned advantages, application of MEOR has been limited because of the lack of practical convincing experimental results. In this study, the effects of MEOR process on wettability changes and the reduction of residual oil saturation have been examined by providing microscopic visualization of two phase flow in transparent glass micromodels. Biosurfactant producing bacterial strain (Enterobacter cloacae) was... 

    Application of biosurfactants to wettability alteration and IFT reduction in enhanced oil recovery from oil-wet carbonates

    , Article Petroleum Science and Technology ; Volume 31, Issue 12 , Jul , 2013 , Pages 1259-1267 ; 10916466 (ISSN) Biria, D ; Maghsoudi, E ; Roostaazad, R ; Sharif University of Technology
    To obtain potentially applicable microorganisms to an effective in situ microbial enhanced oil recovery operation, bacteria that were compatible with the harsh conditions of a petroleum reservoir were isolated from a crude oil sample. The application of an oil spreading technique showed that all of the isolates were capable of producing biosurfactants from both the glucose and crude oil as carbon sources. The secreted biosurfactants could at least reduce the surface tension 20 mN/m and for one of the isolates; the surface tension value dropped below 40 mN/m. In addition, the contact angle measurements revealed that the produced biosurfactants could effectively alter the wettability of the... 

    Modification of rock/fluid and fluid/fluid interfaces during MEOR processes, using two biosurfactant producing strains of Bacillus stearothermophilus SUCPM#14 and Enterobacter cloacae: A mechanistic study

    , Article Colloids and Surfaces B: Biointerfaces ; Vol. 117 , May , 2014 , pp. 457-465 ; ISSN: 09277765 Sarafzadeh, P ; Zeinolabedini Hezave, A ; Mohammadi, S ; Niazi, A ; Ayatollahi, S ; Sharif University of Technology
    During any microbial enhanced oil recovery process, both cells and the metabolic products of bacteria govern the tertiary oil recovery efficiency. However, very accurate examination is needed to find the functionality of these tiny creatures at different reservoir conditions. In this regard, the effect of cell structure on ultimate microbial recovery efficiency which is the most dominant mechanism based on the microorganism types (gram-negative or gram-positive) was systematically investigated. At the first stage, possible different active mechanisms using Bacillus stearothermophilus SUCPM#14 strain were tested using specially designed injection protocol, in situ and ex situ core flooding... 

    Core flooding tests to investigate the effects of IFT reduction and wettability alteration on oil recovery during MEOR process in an Iranian oil reservoir

    , Article Applied Microbiology and Biotechnology ; Volume 97, Issue 13 , July , 2013 , Pages 5979-5991 ; 01757598 (ISSN) Rabiei, A ; Sharifinik, M ; Niazi, A ; Hashemi, A ; Ayatollahi, S ; Sharif University of Technology
    Microbial enhanced oil recovery (MEOR) refers to the process of using bacterial activities for more oil recovery from oil reservoirs mainly by interfacial tension reduction and wettability alteration mechanisms. Investigating the impact of these two mechanisms on enhanced oil recovery during MEOR process is the main objective of this work. Different analytical methods such as oil spreading and surface activity measurements were utilized to screen the biosurfactant-producing bacteria isolated from the brine of a specific oil reservoir located in the southwest of Iran. The isolates identified by 16S rDNA and biochemical analysis as Enterobacter cloacae (Persian Type Culture Collection (PTCC)...