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microfluidic-techniques
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Production of uniform size cell-enclosing silk derivative vehicles through coaxial microfluidic device and horseradish crosslinking reaction
, Article European Polymer Journal ; Volume 172 , 2022 ; 00143057 (ISSN) ; Hosseini, M ; Varaa, N ; Mahmoodi, N ; Goodarzi, A ; Taghdiri Nooshabadi, V ; Hassanzadeh, S ; Arabpour, Z ; Khanmohammadi, M ; Sharif University of Technology
Elsevier Ltd
2022
Abstract
Silk fibroin (SF) as a natural biopolymer holds great potential in biomedical research because of its biocompatibility, easy processability and high strength properties. However, slow gelation time has narrowed its applications, specifically in cell-laden microparticle production due to insufficient crosslinkable moieties. This study aimed to develop cell-laden silk fibroin-phenol (SF-Ph) microparticle through co-flow microfluidic system using SF conjugated Ph moieties whereas covalent crosslinking is mediated with horseradish peroxidase (HRP) in the presence of hydrogen peroxide (H2O2). For this, the SF-Ph was synthesized through carbodiimide condensation crosslinking reaction. Aqueous...
Spheroids-on-a-chip: recent advances and design considerations in microfluidic platforms for spheroid formation and culture
, Article Sensors and Actuators, B: Chemical ; Volume 263 , 15 June , 2018 , Pages 151-176 ; 09254005 (ISSN) ; Kashaninejad, N ; Ebrahimi Warkiani, M ; Lock, J. G ; Moghadas, H ; Firoozabadi, B ; Saidi, M. S ; Nguyen, N. T ; Sharif University of Technology
Elsevier B.V
2018
Abstract
A cell spheroid is a three-dimensional (3D) aggregation of cells. Synthetic, in-vitro spheroids provide similar metabolism, proliferation, and species concentration gradients to those found in-vivo. For instance, cancer cell spheroids have been demonstrated to mimic in-vivo tumor microenvironments, and are thus suitable for in-vitro drug screening. The first part of this paper discusses the latest microfluidic designs for spheroid formation and culture, comparing their strategies and efficacy. The most recent microfluidic techniques for spheroid formation utilize emulsion, microwells, U-shaped microstructures, or digital microfluidics. The engineering aspects underpinning spheroid formation...