Sharif Digital Repository

Polymerase chain reaction, abbreviated as PCR, is a method of amplifying the number of copies of a desired DNA sequence through special protocols. The rapid advance of microfluidic devices and the emerging concept of digital microfluidics has resulted into the development of digital droplet PCR (ddPCR) systems with their significant uses in the detection of rare mutations, cancer diagnosis, and surveillance. A large number of micron-sized droplets are required to perform ddPCR. in this study, To investigate the gradient of confinements induced droplet self-breakup mechanism, we carried out the computational fluid dynamics (CFD) simulations for the two-phase flow using a commercial software... 

Significant advances in biotechnology have led to the emergence of a cost-effective way with less ethical issues to study disease, organ functions, tumors, and their response to drugs besides studying on animals. Microfluidic devices and organ on a chip (tumor on a chip) were introduced to remove those obstacles. Organ on a chip is a powerful tool for studying different types of tissues and simulating diseases, especially cancers, for biological and medical applications. Organ (tumor) on a chip is considered as a smaller scale of the real organ or tumor and it causes to the real-time study of tissues and their functions more accurately. In this study, to fabricate a droplet-based... 

Emulsions are the intermittent dispersion of liquid droplets that have many applications such as polymerization, dyeing, cosmetics, food industry, etc. Droplets can be created in a variety of ways, but the most important point in emulsification and the greatest efficiency of its applications is when the formed droplets are uniform. In the emulsification process, there is a key parameter called interfacial tension, which affects the behavior of the formed droplets, such as their stability and morphology, and its precise measurement is very effective in the quality control of the mentioned applications. Be. In this research, droplet formation in microfluidic systems due to uniform droplet...