Search for: molecular-imprinting
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    The control of morphological and size properties of carbamazepine-imprinted microspheres and nanospheres under different synthesis conditions

    , Article Journal of Materials Research ; 2013 ; 08842914 (ISSN) Esfandyari Manesh, M ; Javanbakht, M ; Shahmoradi, E ; Dinarvand, R ; Atyabi, F ; Sharif University of Technology
    Potential applications of the molecularly imprinted polymers (MIPs) demand physical configurations in different size ranges. Nowadays, research on MIPs is focused on the development of new or improved morphologies, which involves control and modification of the different parameters during the synthesis. In this study, the effect of different synthesis conditions on the particle size and morphology is investigated. Carbamazepine-imprinted polymers were prepared using precipitation polymerization under various conditions such as: the amounts of cross-linker and functional monomers, initiator, porogen, temperature and time of polymerization. We studied the polymerization conditions to obtain... 

    Electrochemical preparation of a molecularly imprinted polypyrrole modified pencil graphite electrode for the determination of phenothiazine in model and real biological samples

    , Article Talanta ; Volume 144 , November , 2015 , Pages 456-465 ; 00399140 (ISSN) Nezhadali, A ; Rouki, Z ; Nezhadali, M ; Sharif University of Technology
    Elsevier  2015
    Abstract A sensitive electrochemical sensor for determination of phenothiazine (PTZ) was introduced based on molecularly imprinted polymer (MIP) film. A computational study was performed to evaluate the template-monomer geometry and interaction energy in the prepolymerization mixture. The electrode was prepared during electropolymerization of pyrrole (Py) on a pencil graphite electrode (PGE) by cyclic voltammetry (CV) technique. The quantitative measurements were performed using differential pulse voltammetry (DPV) in Britton-Robinson (BR) buffer solutions using 60% (v/v) acetonitrile-water (ACN/H2O) binary solvent. The effect of important parameters like pH, monomer... 

    Recent advances in capillary microextraction

    , Article TrAC - Trends in Analytical Chemistry ; Volume 73 , November , 2015 , Pages 64-80 ; 01659936 (ISSN) Bagheri, H ; Piri Moghadam, H ; Sharif University of Technology
    Elsevier  2015
    This review is mostly focused on the recently emerged methodologies for preparing the appropriate extracting media with enhanced capacity, stability and selectivity, which could be used in capillary microextraction (CME). We particularly consider conductive polymers, sol-gel-based materials, monolithic coatings, molecularly-imprinted polymers and xerogels. Moreover, we discuss recent advances in configuration of CME, array capillaries and unbreakable substrates  

    Novel Approach Towards Mechanical and Thermal Resistant Capillary Microextraction by Sol-Gel Technique in On-Line Combination with High Performance Liquid Chromatography

    , Ph.D. Dissertation Sharif University of Technology Piri-Moghadam, Hamed (Author) ; Bagheri, Habib (Supervisor)
    In this thesis, a new approach was developed to achieve mechanical and chemical resistant capillary microextraction (CME) in on-line combination with high performance liquid chromatography (HPLC) based on sol-gel technology. This thesis is divided into three chapters:
    First chapter contains of two parts. Overcoming the lack of mechanical stability of the substrate of CME by replacing the fused silica by copper tubings was the main objective of the first part. Self-assembled monolayers technique was employed to functionalize the copper tube followed by chemical bonding of the sorbent on the inner surface of the copper tube by sol-gel technology. The prepared copper tube containing the... 

    Cell Separation via Nano-porous Molecularly Imprinted Polymer

    , M.Sc. Thesis Sharif University of Technology Rahnema Falavarjani, Eiman (Author) ; Abdekhodaie, Mohammad Jafar (Supervisor) ; Nematollah Zade, Ali (Co-Advisor)
    Molecularly imprinted polymers (MIPs) with synthetic recognition sites are capable of detecting and separating chemical species in complex environments. These characteristics are of increasing interest for bioanalytical applications. The chief advantages of MIPs are mechanical and chemical stability, low cost, and ease of preparation. Recently, imprinting technology has been used for fabrication polymer thin films with recognition sites of proteins, plant viruses, and yeasts. Also few investigations applied lithography for synthesis of cell imprinted polymers targeting cell detection and observation of cell morphology.
    For the first time, we demonstrate surface imprinting for the... 

    A Novel Magnetic Molecularly Imprinted Polymers Nanoparticle as Extracting Medium For Micro-Solid Phase Extraction

    , M.Sc. Thesis Sharif University of Technology Yahya Baktash, Mohammad (Author) ; Bagheri, Habib (Supervisor)
    This thesis cantain two section. In the first section a novel extracting medium based on magnetic molecularly imprinted polymers (MIPs) nanoparticles was synthesized by chemical polymerization for micro-solid phase extraction. In this work, the modified chitosan magnetite nanoparticles (MNP-CS) was synthesized by co-precipitating Fe2+ and Fe3+ in the chitosan solution [1,2]. The characteristic properties of Fe3O4@MIPs nanoparticles were determined using Fourier transform infrared spectrometer. Extraction efficiency of the novel extracting medium was compared with MNP-CS sorbents for the determination of RhB in aqueous samples, via quantification by spectrofluorimetry. The obtained results... 

    Application of sol-gel based molecularly imprinted xerogel for on-line capillary microextraction of fentanyl from urine and plasma samples

    , Article Analytical Methods ; Volume 5, Issue 24 , 2013 , Pages 7096-7101 ; 17599660 (ISSN) Bagheri, H ; Piri-Moghadam, H ; Bayat, P ; Balalaie, S ; Sharif University of Technology
    A molecularly imprinted xerogel (MIX) for fentanyl was successfully prepared on the inner surface of a copper tube by sol-gel technology. Primarily, the tube was treated by self-assembly monolayers of 3-(mercaptopropyl) trimethoxysilane and then [3-(2,3-epoxypropoxy)-propyl]-trimethoxysilane was employed as a precursor for imprinting the template molecule in acidic conditions. During the sol-gel process influential parameters including volume and concentration of fentanyl were carefully investigated to obtain the most efficient and appropriate xerogel. In parallel, a non-imprinted xerogel (NIX) was synthesized in the absence of fentanyl. The extraction efficiencies achieved from MIX and NIX,... 

    Towards greater mechanical, thermal and chemical stability in solid-phase microextraction

    , Article TrAC - Trends in Analytical Chemistry ; Volume 34 , 2012 , Pages 126-138 ; 01659936 (ISSN) Bagheri, H ; Piri-Moghadam, H ; Naderi, M ; Sharif University of Technology
    Solid-phase microextraction (SPME) is a fast, solvent-free technique, which, since its introduction in the 1990s, has been increasingly applied to sample preparation in analytical chemistry. Conventional SPME fibers are fabricated by making a physical bond between the usual silica substrate and the polymeric coatings. However, some applications are limited, as the lifetime and the stability of conventional SPME fibers cannot meet the demands of analyzing relatively non-volatile compounds with more polar moieties. There have been attempts to analyze less volatile compounds by increasing the thermal, physical and chemical stability of the fibers. In this review, we present some new... 

    Sol-gel-based molecularly imprinted xerogel for capillary microextraction

    , Article Analytical and Bioanalytical Chemistry ; Volume 404, Issue 5 , September , 2012 , Pages 1597-1602 ; 16182642 (ISSN) Bagheri, H ; Piri-Moghadam, H ; Sharif University of Technology
    Springer  2012
    A novel molecularly imprinted xerogel (MIX) based on organically modified silica (ORMOSIL) was successfully prepared for on-line capillary microextraction (CME) coupled with high-performance liquid chromatography (HPLC). The sol-gel-based xerogel was prepared using only one precursor and exhibited extensive selectivity towards triazines along with significant thermal and chemical stability. Atrazine was selected as a model template molecule and 3-(trimethoxysilyl)propylmethacrylate (TMSPMA) as a precursor in which the propylmethacrylate moiety was responsible for van der Waals, dipole-dipole, and hydrogen-bond interactions with the template. This moiety plays a key role in creation of... 

    High-capacity hierarchically imprinted polymer beads for protein recognition and capture

    , Article Angewandte Chemie - International Edition ; Volume 50, Issue 2 , December , 2011 , Pages 495-498 ; 14337851 (ISSN) Nematollahzadeh, A ; Sun, W ; Aureliano, C. S. A ; Lütkemeyer, D ; Stute, J ; Abdekhodaie, M. J ; Shojaei, A ; Sellergren, B ; Sharif University of Technology
    Leaving an imprint: Hierarchical protein imprinting starting from wide-pore silica modified with a submonolayer of adsorbed protein (IgG or HSA) leads to an inverse polymeric replica of the silica template that features highly accessible protein-complementary binding sites (see picture). The resulting poly(acrylamide) beads (PIgG or PHSA) feature high binding capacities and can be used to selectively capture the proteins HSA and IgG from blood serum  

    Evaluation of molecular imprinted polymerized methylene blue/aptamer as a novel hybrid receptor for cardiac troponin I (cTnI) detection at glassy carbon electrodes modified with new biosynthesized ZnONPs

    , Article Sensors and Actuators, B: Chemical ; Volume 320 , 1 October , 2020 Mokhtari, Z ; Khajehsharifi, H ; Hashemnia, S ; Solati, Z ; Azimpanah, R ; Shahrokhian, S ; Sharif University of Technology
    Elsevier B.V  2020
    In this research, a novel, rapid, and non-immune electrochemical method was used to detect cardiac troponin I (cTnI) using a double recognition approach. Amine terminus cTnI aptamers immobilized on COOH-ZnO nanoparticles (COOH-ZnONPs) modified GCE surface were applied to capture cTnI for imprinting recognition. The COOH-ZnONPs were synthesized in a biological manner. Then, the methylene blue (MB) monomers were electro-polymerized around the cTnI-aptamer complexes. Following the removal of cTnI, cavities were constructed and converted to a new aptamer and molecular imprinted polymer (MIP) hybrid receptor (aptamer/MIP/ZnONPs). FT-IR spectra, SEM images, XRD patterns, and electrochemical... 

    An imprinted interpenetrating polymer network for microextraction in packed syringe of carbamazepine

    , Article Journal of Chromatography A ; Volume 1491 , 2017 , Pages 1-8 ; 00219673 (ISSN) Asgari, S ; Bagheri, H ; Es haghi, A ; Amini Tabrizi, R ; Sharif University of Technology
    An imprinted interpenetrating polymer network (IPN) was synthesized and used as a medium for isolation of carbamazepine from urine samples. The polymer network consisted of a homogeneous polystyrene–sol gel hybrid constructed by in–situ radical polymerization method. In this process, within the sol–gel reaction duration, styrene monomer could penetrate into the reaction mixture and after the polymerization initiation, a monolithic IPN structure was prepared. The scanning electron microscopy (SEM) image and energy dispersive spectroscopy (EDX) are indications of the polystyrene dispersion at nano- to micro-meter level in the sol gel matrix. Eventually, the synthesized IPN was used as a... 

    Synthesis of Molecularly Imprinted Nano-porous Polymer for the Enrichment of Taxol as Anti-Cancer Agent

    , M.Sc. Thesis Sharif University of Technology Shirzaei Sani, Ehsan (Author) ; Abdekhodaie, Mohammad Jafar (Supervisor) ; Nematollahzadeh, Ali (Co-Advisor) ; Musavi, Abbas (Co-Advisor)
    In the present research, first, a series of molecular dynamics (MD) simulations of pre-polymerization mixtures for taxol (template) imprinted polymers was performed to select an appropriate monomer and its intermolecular bonds with the template. Finally, after synthesis, performance and morphological evaluation of taxol imprinted polymer were presented. In the molecular modeling step, a virtual library of functional monomers was created containing eleven frequently used monomers. The MD simulations were first conducted to select the optimum monomer from the virtual library using acetonitrile as porogen. Methacrylic acid among the studied functional monomers was found to possess stronger... 

    Molecularly Imprinted Polymers for the Treatment of Disease

    , Ph.D. Dissertation Sharif University of Technology Nematollahzadeh, Ali (Author) ; Abd Khodaei, Mohammad (Supervisor) ; Shojaei, Akbar (Supervisor)
    In the present investigation, experimental design, synthesis and evaluation of Verapamil, Albumin, Immonuglubline G and Trypsin imprinted polymers and finally Albumin imprinted poly(dopamine) nano-layer were presented. A bimodal polymerization approach as a combination of precipitation and dispersion polymerization was taken towards producing 500 nm particles for verapamil imprinting purpose. In this case the imprinting factor was 2.17. In a challenge for producing spherical particles with predefined size and pore properties the hierarchical polymerization in packed beds, using porous silica particles (particle size ca. 40 µm) was developed, as well. The method resolving the problems... 

    Imprinted silica nanofiber formation via sol-gel-electrospinning for selective micro solid phase extraction

    , Article New Journal of Chemistry ; Volume 42, Issue 16 , 2018 , Pages 13864-13872 ; 11440546 (ISSN) Asgari, S ; Bagheri, H ; Es Haghi, A ; Sharif University of Technology
    Royal Society of Chemistry  2018
    Sol composition and ripening conditions are two important parameters in silica fiber production through the conventional sol-gel process. In the current study, silica nanofibers were successfully obtained via the electrospinning technique, and sol-gel formation occurred during this process. Based on previous reports, with the aim of increasing the selectivity, we used the molecular imprinted methodology to produce a selective medium via sol-gel electrospinning. In this process, sol-gel was formed during electrospinning and then, the backbone polymer was removed by heating. To obtain a thin layer of silica nanofibers, the influencing parameters such as backbone polymer types, imprinting time... 

    Molecularly imprinted polydopamine nano-layer on the pore surface of porous particles for protein capture in HPLC column

    , Article Journal of Colloid and Interface Science ; Volume 404 , 2013 , Pages 117-126 ; 00219797 (ISSN) Nematollahzadeh, A ; Shojaei, A ; Abdekhodaie, M. J ; Sellergren, B ; Sharif University of Technology
    Bio-inspired Human Serum Albumin (HSA) imprinted polydopamine nano-layer was produced through oxidative polymerization of dopamine on the pore surface of HSA modified porous silica particles. The coating thickness was controlled by the reaction time and thereby varied within 0-12. nm. The samples were characterized by elemental analysis, FT-IR, DSC, SEM, TEM, TGA, physisorption and thermoporometry. The characterization confirmed the success of evolution and deposition of polydopamine layer on the silica pore surface. Batch rebinding experiment showed that the molecularly imprinted polymer (MIP) with 8.7. nm coating thickness, in comparison with the thinner and thicker coatings, displays the... 

    Novel Sol Gel– and Electrospun– Based Nanostructure Sorbents for Extraction of Drugs and Pollutants

    , Ph.D. Dissertation Sharif University of Technology Asgari, Sara (Author) ; Bagheri, Habib (Supervisor)
    The main purpose of this dissertation was to synthesize nanostructured adsorbents based on the sol–gel and electrospinning methods and implement them to the trace determination of the desired analytes from different matrices. In the first and second chapters, a summary of the sol–gel and electrospinning methods along with a glance on extraction methods are presented. Then, the implemented projects are presented in the consecutive chapters.In the first work, third chapter, silica nanofibers were successfully produced using electrospinning method. In this process, sol–gel was formed during electrospinning and then, the backbone polymer was removed by heating. To obtain a thin layer of silica... 

    Development of Biocompatible and Fluorescent Platforms Based on Carbon Dots and Deep Eutectic Solvents for Detection of Some Carcinogenic and Antidepressant Compounds

    , Ph.D. Dissertation Sharif University of Technology Manshaei, Faranak (Author) ; Bagheri, Habib (Supervisor)
    The main focus of this thesis was on fluorescence sensors development based on carbon dots and deep eutectic solvents. Accordingly, an attempt was made to synthesize carbon dots and modified with deep eutectic solvents as biocompatible sensors. Eventually, development and application of these sensors in analytical systems ultimately led to satisfactory and comparable results with previous methods. In the first project, a rapid and miniaturized fluorescent probe based on the ultrasound‒assisted formation of carbon dots (CDs) in a metallic deep eutectic solvent (MDES) was developed and conveniently implemented to detect 4-MeI in commercial dark soft drinks. In-situ synthesis of CDs-chelated... 

    Paclitaxel molecularly imprinted polymer-PEG-folate nanoparticles for targeting anticancer delivery: Characterization and cellular cytotoxicity

    , Article Materials Science and Engineering C ; Volume 62 , 2016 , Pages 626-633 ; 09284931 (ISSN) Esfandyari Manesh, M ; Darvishi, B ; Azizi Ishkuh, F ; Shahmoradi, E ; Mohammadi, A ; Javanbakht, M ; Dinarvand, R ; Atyabi, F ; Sharif University of Technology
    Elsevier Ltd  2016
    The aim of this work was to synthesize molecularly imprinted polymer-poly ethylene glycol-folic acid (MIP-PEG-FA) nanoparticles for use as a controlled release carrier for targeting delivery of paclitaxel (PTX) to cancer cells. MIP nanoparticles were synthesized by a mini-emulsion polymerization technique and then PEG-FA was conjugated to the surface of nanoparticles. Nanoparticles showed high drug loading and encapsulation efficiency, 15.6 ± 0.8 and 100%, respectively. The imprinting efficiency of MIPs was evaluated by binding experiments in human serum. Good selective binding and recognition were found in MIP nanoparticles. In vitro drug release studies showed that MIP-PEG-FA have a... 

    Design of a pseudo stir bar sorptive extraction using graphenized pencil lead as the base of the molecularly imprinted polymer for extraction of nabumetone

    , Article Spectrochimica Acta - Part A: Molecular and Biomolecular Spectroscopy ; Volume 238 , 2020 Afsharipour, R ; Dadfarnia, S ; Haji Shabani, A. M ; Kazemi, E ; Sharif University of Technology
    Elsevier B.V  2020
    Molecularly imprinted polymer (MIP) was synthesized through the coprecipitation method on the graphene oxide anchored pencil lead as a substrate for the first time and applied as an efficient sorbent for pseudo stir bar sorptive extraction of nabumetone. The extracted analyte was determined by a novel spectrophotometric method based on the aggregation of silicate sol-gel stabilized silver nanoparticles in the presence of the analyte. The synthesized polymer was characterized using Fourier transform infrared spectroscopy and field emission scanning electron microscopy. Optimization of important parameters affecting the extraction efficiency was done using central composite design whereas the...