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The Impedimetric Human Papiloma Virous DNA Biosensor Fabrication Based on Gold Nanotubes
, Ph.D. Dissertation Sharif University of Technology ; Ghorbani, Mohammad (Supervisor) ; Sasanpour, Pezhman (Supervisor)
Abstract
An impedimetric human papilloma virus (HPV) DNA biosensor based on gold nanotubes (AuNTs) in label free detection was materialized. The AuNTs decorated nanoporous polycarbonate (AuNTs-PC) template as biosensor electrode was fabricated by electrodeposition method. The single strand DNA (ss-DNA) probe was covalently immobilized onto the AuNTs-PC electrode. The hybridization of target sequences with the ss-DNA probe was observed by the electrochemical impedance spectroscopy (EIS). The biosensor showed high selectivity and could differentiate between the complementary, mismatch and non-complementary DNA sequences. The EIS measurements were matched to Randle's equivalent circuit. The...
Fabrication of Porous Electrospun Fibe on Biocompatible Polymers such as Polycaprolactone (Pcl) and Thrs Based eir Application in Sample Preparation
, M.Sc. Thesis Sharif University of Technology ; Bagheri, Habib (Supervisor)
Abstract
The aim of this study was to develop a method to determine the trace residual amounts of nonsteroidal anti-inflammatory drugs in meat-related samples and to study the effect of different cooking methods and storage conditions of meat before cooking as an effective factor on the fate of these drugs. To proceed this goal, a nanocomposite was prepared from polycaprolactone nanofiber/silica mesopores as green sorbent by electrospinning. The synthesized nanocomposite was implemented for micro-solid phase extraction of the desired drugs in conjunction with high performance liquid chromatography. Polycaprolactone, due to its excellent physical properties, availability, biodegradability,...
An ultrasensitive label free human papilloma virus DNA biosensor using gold nanotubes based on nanoporous polycarbonate in electrical alignment
, Article Analytica Chimica Acta ; 2018 ; 00032670 (ISSN) ; Ghorbani, M ; Sasanpour, P ; Karimizefreh, A ; Sharif University of Technology
Elsevier B.V
2018
Abstract
An impedimetric human papilloma virus (HPV) DNA biosensor based on gold nanotubes (AuNTs) in label free detection was materialized. The AuNTs decorated nanoporous polycarbonate (AuNTs-PC) template as biosensor electrode was fabricated by electrodeposition method. The single strand DNA (ss-DNA) probe was covalently immobilized onto the AuNTs-PC electrode. The hybridization of target sequences with the ss-DNA probe was observed by the electrochemical impedance spectroscopy (EIS). The biosensor showed high selectivity and could differentiate between the complementary, mismatch and non-complementary DNA sequences. The EIS measurements were matched to Randle's equivalent circuit. The...
An ultrasensitive label free human papilloma virus DNA biosensor using gold nanotubes based on nanoporous polycarbonate in electrical alignment
, Article Analytica Chimica Acta ; Volume 1048 , 2019 , Pages 31-41 ; 00032670 (ISSN) ; Ghorbani, M ; Sasanpour, P ; Karimizefreh, A ; Sharif University of Technology
Elsevier B.V
2019
Abstract
An impedimetric human papilloma virus (HPV) DNA biosensor based on gold nanotubes (AuNTs) in label free detection was materialized. The AuNTs decorated nanoporous polycarbonate (AuNTs-PC) template as biosensor electrode was fabricated by electrodeposition method. The single strand DNA (ss-DNA) probe was covalently immobilized onto the AuNTs-PC electrode. The hybridization of target sequences with the ss-DNA probe was observed by the electrochemical impedance spectroscopy (EIS). The biosensor showed high selectivity and could differentiate between the complementary, mismatch and non-complementary DNA sequences. The EIS measurements were matched to Randle's equivalent circuit. The...