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Site-specific protein conjugation onto fluorescent single-walled carbon nanotubes
, Article Chemistry of Materials ; Volume 32, Issue 20 , 2020 , Pages 8798-8807 ; Wu, S. J ; Rahnamaee, S. Y ; Schuergers, N ; Boghossian, A. A ; Sharif University of Technology
American Chemical Society
2020
Abstract
Semiconducting single-walled carbon nanotubes (SWCNTs) are among the few photostable optical emitters that are ideal for sensing, imaging, drug delivery, and monitoring of protein activity. These applications often require strategies for immobilizing proteins onto the nanotube while preserving the optical properties of the SWCNTs. Site-specific and oriented immobilization strategies, in particular, offer advantages for improving sensor and optical signaling responses. In this study, we demonstrate site-specific protein immobilization of a model of enhanced yellow fluorescent protein with a single engineered cysteine residue, using either single-stranded DNA or a pyrene-containing linker to...