Sharif Digital Repository

Concentration gradient generators (CGGs) help biologists to perform large scale, fast and high-throughput experiments. This paper introduces a design tool called Tree-like Concentration gradient generator Design Tool (TCDT). The performance of this tool is validated both numerically and experimentally. Three CGGs were fabricated using three different fabrication methods and design parameters. The performance of these devices was examined using the measurement of fluorescent and dye intensity. The performance of the design tool for non-linear and multi-drug concentration gradient generations was investigated as well. In addition, a method was developed to investigate the multi-drug... 

In addition to perfectly steering the output concentration of a process network to an exogenous set-point, a desired synthetically implemented biological controller should be able to robustly maintain this regulated output in the face of the extrinsic disturbances and inherent uncertainties due to an evervarying environment besides the imprecise modeling. Such an ability, which is called robust perfect adaptation (RPA), can be achieved by integral feedback control (IFC). Answering how IFC is (biochemically) constructible in generally unknown synthetic networks has been a research focus in the community. One of these answers, which has been well investigated previously, is to utilize a simple...