Search for: oxidized-alginates
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    Preparation of Gelatin-Alginate Hydrogel for Using as Cell Culture Scaffold

    , M.Sc. Thesis Sharif University of Technology Fadaodini, Samira (Author) ; Mashayekhan, Shohreh (Supervisor) ; Maghsoudi, Vida (Supervisor)
    Hydrogels are polymeric three-dimensional networks able to swell in the presence of an aqueous medium. Hydrogels from natural proteins and polysaccharides are ideal scaffolds for tissue engineering since they resemble the extracellular matrices of tissue comprised of various amino acids and sugar-based macromolecules.The biocompatible and biodegradable hydrogel scaffolds are promising materials for tissue engineering. Here, we report a new class of hydrogels derived from oxidized alginate (OA) and gelatin. The prepared oxidized alginate was shown to be efficient in crosslinking gelatin, leading to hydrogel formation. The effect of degree of oxidation and concentration of OA on the mechanical... 

    Design, fabrication and characterization of oxidized alginate-gelatin hydrogels for muscle tissue engineering applications

    , Article Journal of Biomaterials Applications ; Volume 31, Issue 1 , 2016 , Pages 152-161 ; 08853282 (ISSN) Baniasadi, H ; Mashayekhan, S ; Fadaoddini, S ; Haghirsharifzamini, Y ; Sharif University of Technology
    SAGE Publications Ltd  2016
    In this study, we reported the preparation of self cross-linked oxidized alginate-gelatin hydrogels for muscle tissue engineering. The effect of oxidation degree (OD) and oxidized alginate/gelatin (OA/GEL) weight ratio were examined and the results showed that in the constant OA/GEL weight ratio, both cross-linking density and Young's modulus enhanced by increasing OD due to increment of aldehyde groups. Furthermore, the degradation rate was increased with increasing OD probably due to decrement in alginate molecular weight during oxidation reaction facilitated degradation of alginate chains. MTT cytotoxicity assays performed on Wharton's Jelly-derived umbilical cord mesenchymal stem cells... 

    Synthesis, Characterization and Optimization of an In situ Forming Hydrogel Derived from Extracellular Matrix for Cardiac Tissue Engineering Applications

    , M.Sc. Thesis Sharif University of Technology Mousavi, Ali (Author) ; Mashayekhan, Shohreh (Supervisor) ; Baheiraei, Nafiseh (Supervisor) ; Pourjavadi, Ali (Co-Supervisor)
    Myocardial Infarction occurs due to sudden blockage in a coronary artery and causes necrosis of myocardial tissue. Since myocardium is unable to self-regenerate, cardiac tissue engineering has become a promising therapeutic approach for MI treatment by restoring heart function via combination of cells, biomaterials and signaling factors. For this purpose, myocardial extracellular matrix (ECM) is an attractive biomaterial providing better biomimetic for cultured cells. In this project, we synthesized an in situ forming hydrogel derived from myocardial ECM and for improving mechanical and electrical properties of ECM hydrogel we added oxidized alginate (OA) with 5% oxidation degree and APTMS... 

    Design and Fabrication of Mineral-based Porous Microcarrier for Bone Tissue Regeneration

    , M.Sc. Thesis Sharif University of Technology Haji Abbas, Mohammad Ali (Author) ; Mashayekhan, Shohreh (Supervisor) ; Bahrevari, Mohammad Reza (Co-Supervisor)
    Currently, using biocompatible and injectable polymeric microcarriers as one of the efficient methods to transfer cells and active agents has gained much attention for bone regenerative medicine. However, they have some drawbacks such as weak mechanical stability and lack of mineral materials, which are the major ingredients of the bone tissues. Accordingly, it is expected that mimicking the chemical and physical structure of bone tissues could be valuable in their medical applications. Herein, a new porous biodegradable microcarriers (MCs) made of silk fibroin-oxidized alginate-bioactive glass was fabricated by electrospraying method. Response surface methodology (RSM) was used to study the... 

    Production of drug Loaded Microgels Using Microfluidic

    , M.Sc. Thesis Sharif University of Technology Mehraji, Sima (Author) ; Saadatmand, Maryam (Supervisor) ; Eskandari, Mahnaz (Supervisor)
    Microfluidic systems have the ability to produce microgels with uniform size distribution and spherical shape due to the laminar flow and better control of the flow rates. Spherical microgels with same size can be used as high-potential carriers for drug release. An important challenge in the production of these drug-carrying microgels is the simultaneous process of drug loading into microgels and their production. The overall goal of this project was to produce drug-carrying microgels with uniform size distribution that show controlled drug release. To achieve this goal, various factors such as the type of polymer, the type of drug, the volume ratio of the polymer and the drug and the... 

    Fabrication of Thick Scaffold with Microfluidic Channels by Bioprinter

    , M.Sc. Thesis Sharif University of Technology Khalighi, Sadaf (Author) ; Saadatmand, Maryam (Supervisor)
    Cardiovascular diseases are among the leading causes of death worldwide. For instance, in 2015, almost 31% of the world’s mortality rate was due to these causes. One of these diseases is cardiac coronary vessels’ occlusion which leads to the insufficient blood supply to the heart tissue and cardiomyocytes death after Myocardial Infarction (MI). After MI, a hierarchy of events in the heart tissue changes heart muscle and forms cardiac fibrosis. This fibrotic tissue does not have the native one’s properties and function, so it will cause cardiac arrest and patient death. Therefore, it is obvious that vascular network plays a crucial role in the heart function. The importance of cardiac... 

    Design of Amniotic Membrane-based Hydrogel for Cardiac Tissue Engineering Application

    , M.Sc. Thesis Sharif University of Technology Gholami, Bahar (Author) ; Yaghmaei, Sohila (Supervisor) ; Saadatmand, Maryam (Supervisor)
    Mammalian cardiac tissue lacks the ability to effectively self-regenerate following severe damage. The application of external therapeutic agents with strong mechanical properties is needed to restore its function. Even though conventional therapies have several challenges and limitations, injectable hydrogels, with minimally invasive, can significantly improve cardiac tissue regeneration. Extracellular matrices are the most appropriate biomaterials for synthesizing cardiac scaffolds. The human amniotic membrane obtained from the amniotic sac is a readily available, abundant, and inexpensive candidate that has been successfully utilized for the clinical treatment of cardiac diseases.... 

    Study and Fabrication of a Multilayer Scaffold Containing Biological Agents for Skin Wounds Regeneration

    , Ph.D. Dissertation Sharif University of Technology Hajiabbas, Maryam (Author) ; Alemzadeh, Iran (Supervisor) ; Vossoughi, Manouchehr (Supervisor) ; Shamloo, Amir (Co-Supervisor)
    In recent years, it is expected that the fabrication of multilayer scaffolds and the use of different methodologies in one product can be a new progressing method in skin substitute production. Accordingly, this project aims to fabricate a bilayered composite scaffold with a combination of hydrogel and electrospinning method. We have tried to prepare a scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) without using corrosive solvents and toxic crosslinking agents as a scaffold and drug delivery system. As different biological, chemical, physical, and mechanical factors play a vital role in the healing process, we have characterized the proposed scaffold via DSC,... 

    A porous hydrogel-electrospun composite scaffold made of oxidized alginate/gelatin/silk fibroin for tissue engineering application

    , Article Carbohydrate Polymers ; Volume 245 , 2020 Hajiabbas, M ; Alemzadeh, I ; Vossoughi, M ; Sharif University of Technology
    Elsevier Ltd  2020
    In the article, a bilayer nanocomposite scaffold made of oxidized alginate (OAL), gelatin (G), and silk fibroin (SF) has been prepared via combining electrospinning, in situ gas foaming, in situ crosslinking and freeze drying methods. The physicochemical and mechanical properties, as well as thermal stability of the proposed composite, have been investigated by SEM, FTIR, XRD, tensile, and TGA analysis. The data indicate that structure and degree of crosslinking play a vital role in adjusting the physical and mechanical properties of composite scaffolds. Further, the authors find a favorable adipose-derived mesenchymal stem cell's (AMSC) attachment and distribution within this novel...