Loading...
Search for:
phenol-red
0.004 seconds
Purification and zymography of lipase from aspergillus Niger PTCC5010
, Article International Journal of Engineering, Transactions B: Applications ; Volume 28, Issue 8 , 2015 , Pages 1115-1123 ; 1728-144X (ISSN) ; Alemzadeh, I ; Tabatabaee Yazdi, F ; Vossoughi, M ; Varidi, M ; Sharif University of Technology
Materials and Energy Research Center
2015
Abstract
In this study, Lipase from Aspergillus Niger after extraction of medium culture was precipitated with different percentages of acetone and purified by ion exchange chromatography using SP-Sepharose HP and Q-Sepharose HP. The process of purification of the enzyme was studied by electrophoresis and the molecular weight was detected and determined by Zymography using overlying containing phenol red and Rhodamine B. The results showed that the vast majority of lipase from this strain has been precipitated by 70% saturation acetone, and leads to the 1.67 fold the purified enzyme, with special activities 32.8 U. mg-1 and efficiency of 38.5%. Using two-phase chromatography, enzyme specific activity...
Modeling the Removal of Phenol Dyes Using a Photocatalytic Reactor with SnO2/Fe3O4 Nanoparticles by Intelligent System
, Article Journal of Dispersion Science and Technology ; Volume 36, Issue 4 , Apr , 2015 , Pages 540-548 ; 01932691 (ISSN) ; Hedayati Moghaddam, A ; Nouri, A ; Shayegan, J ; Sharif University of Technology
Taylor and Francis Inc
2015
Abstract
The objective of this study was to model the extent of improvement in the degradability of phenol dyes by SnO2/Fe3O4 nanoparticles using a photocatalytic reactor. The effect of operative parameters including catalyst concentration, initial dye concentration, stirring intensity, and UV radiation intensity on the photocatalytic batch reactor during removal of phenol red was investigated. Fractional factorial design and response surface methodology were used to design the experiment layout. The SnO2/Fe3O4 nanoparticles were synthesized using the core-shell method. The results of x-ray diffraction and transmission electron microscopy showed the successful synthesis of these nanoparticles. The...