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polyhydroxyalkanoates
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Optimization of microbial culture for production of poly hydroxy butyrate (PHB) by azotobacter beijerinckii DSMZ-1041
, Article World Applied Sciences Journal ; Volume 14, Issue SPL ISS 3 , 2011 , Pages 72-82 ; 18184952 (ISSN) ; Ardjmand, M ; Nasab, A. H ; Kordi, A. S ; Sharif University of Technology
Abstract
Azotobacter beijerinckii DSMZ 1041 strain was used for production of polyhydroxybutyrate (PHB) and the optimum glucose concentration, as the carbon source and ammonium chloride, as the nitrogen source were obtained 60gr/lit and 0.5gr/lit respectively. The resulting optimum PHB production was investigated 2.95gr/lit. The obtained yield of PHB with respect to glucose (Yp/s) and PHB productivity (Qp) were 0.202 g/g and 0.123 g/lit.hr, respectively. The response surface methodology (RSM) was used to verify the accuracy of the designed experiments. azotobacter beijerinckii microorganism from Deutsch sammlung von microorganismen und zellkulturen(DSMZ) bank is used for production of Poly Hydroxy...
Investigation of optimum fermentation condition for PHA production by four species: Hydrogenophaga pseudoflava DSMZ 1034, azohydromonas lata DSMZ 1123, cupriavidus necator DSMZ 545, Azotobacter beijinckii DSMZ 1041
, Article World Applied Sciences Journal ; Volume 14, Issue SPL ISS 3 , 2011 , Pages 36-47 ; 18184952 (ISSN) ; Ardjmand, M ; Yunesi, H ; Safe Kordi, A ; Yaghmaei, S ; Sharif University of Technology
2011
Abstract
Batch cultures of Azohydromonas lata DSMZ 1123, Hydrogenophaga pseudoflava DSMZ 1034, Cupriavidus necator DSMZ 545 and Azotobacter beijinckii DSMZ 1041 were investigated for producing the intracellular bioplastic poly-•-hydroxybutyrate (PHB). The effects of temperature, seed age, type of carbon sources and the agitation rate on the production rate of PHB were investigated using four mentioned spices. The optimized shaking rate, temperature and seed age were obtained 250 rpm, 30°C and 15 hrs for H. pseudoflava DSMZ 1034 and A. beijerinckii DSMZ 1041 and 18 hrs and 24 hrs for A. lata DSMZ 1123 and C. necator DSM 545, respectively.The effect of carbon sources including glucose, fructose, whey...
Experimental investigation of poly-β-hydroxybutyrate production by azohydromonas lata: Kinetics and artificial neural network modeling
, Article Chemical Engineering Communications ; Volume 203, Issue 2 , 2016 , Pages 224-235 ; 00986445 (ISSN) ; Younesi, H ; Ardjmand, M ; Safe Kordi, A ; Yaghmaei, S ; Qaderi, H ; Sharif University of Technology
Taylor and Francis Ltd
2016
Abstract
Batch culture of Azohydromonas lata was investigated for the production of intracellular poly-b-hydroxybutyrate (PHB). In order to determine the C:N value of the culture media for maximizing the microbial productivity of PHB, different concentrations of glucose and ammonium chloride were used as carbon and nitrogen sources, respectively. The optimal temperature and shaking rate was obtained at 30_C and 180 rpm, respectively. The maximum intracellular PHB concentration obtained was 5.09 g/l, which was 20% (w/w) of the cell dry weight (CDW) after 72 h. Also, the synthesis of PHB was growth associated with the C:N ratio of 153.71. The maximum calculated Yp/s was 0.212 (gr/gr) and the specific...