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Ionic liquid/graphene oxide as a nanocomposite for improving the direct electrochemistry and electrocatalytic activity of glucose oxidase
, Article Journal of Solid State Electrochemistry ; Volume 17, Issue 1 , January , 2013 , Pages 183-189 ; 14328488 (ISSN) ; Ghasemi, S ; Ghourchian, H ; Gholami, M. R ; Sharif University of Technology
2013
Abstract
By combination of 1-ethyl-3-methyl immidazolium ethyl sulfate as a typical room temperature ionic liquid (IL) and graphene oxide (GO) nanosheets, a nanocomposite was introduced for improving the direct electrochemistry and electrocatalytic activity of glucose oxidase (GOx). The enzyme on the IL-GO-modified glassy carbon electrode exhibited a quasireversible cyclic voltammogram corresponding to the flavine adenine dinucleotide/FADH2 redox prosthetic group of GOx. At the scan rate of 100 mV s-1, the enzyme showed a peak-to-peak potential separation of 82 mV and the formal potential of -463 mV (vs Ag/AgCl in 0.1 M phosphate buffer solution, pH 7.0). The kinetic parameters of the charge transfer...