Sharif Digital Repository

Asparagainase is a therapeutic enzyme which has been a subject of research for decades. The enzyme catalyzes the hydrolysis of the amide group in asparagine and similar amides. Altering the substrate specificity and stabilization of this enzyme can increase its therapeutic properties. Moreover, asparaginases may be evolved to catalyze the hydrolysis of other similar compounds. These can be achieved through directed evolution and computational methods.In this study, the gene encoding L-asparaginase II enzyme from E. coli was amplified by polymerase chain reaction (PCR) and was cloned into an expression vector. The recombinant protein was expressed by an appropriate host secreting the... 

Transglutaminases of family enzymes are implicated in different functions in cell including cellular division and cell adhesion. Several members of this family catalyze formation of isopeptide bond that involved in wound restoration and angiogenesis. Transglutaminase 2 that have been studied in this project, are found in almost every human cell tissue. This enzyme has multiple functions depending on its cell location. In cytoplasm TG2 act as GTPase and is involve in post translational modifications. This enzyme by using calcium form a isopeptide bond between two proteins, isopeptide bond is function in brain cortex and cells creates amyloid structures that including brain diseases, Parkinson...