Loading...
Search for:
protein-fibrillation
0.01 seconds
Fabrication and Characterization of a Drug Release System Based on Mesoporous Silica Nanoparticles for Hydrophobic Drugs
,
M.Sc. Thesis
Sharif University of Technology
;
Yaghmaei, Soheila
(Supervisor)
;
Arpanaei, Ayyoob
(Supervisor)
;
Morshedi, Dina
(Co-Advisor)
Abstract
This research aims to develop a drug delivery system based on mesoporous silica nanoparticles (MSNPs) for hydrophobic drugs and evaluating their cytotoxicity. The internal environment of the body is aqueous, while most of effective drugs display poor aqueous solubility, resulting in insufficient bioavailability. Due to their several unique properties, such as a large surface area, tunable pore size, facile surface multi functionalization and excellent biocompatibility, MSNPs are recognized as promising and powerful tools to overcome this hurdle. In the present study, MSNPs were synthesized using template removing method and then were functionalized through grafting procedure. They were...
Design and Fabrication of Drug-loaded Nanoparticles to Prevent Fibrillation of Alpha-synuclein in Parkinson
, M.Sc. Thesis Sharif University of Technology ; Mashayekhan, Shohreh (Supervisor) ; Morshedi, Dina (Supervisor)
Abstract
The purpose of this study is to assess the inhibitory effects of an appropriate nanoparticles loaded with gallic acid on the fibrillation of alpha-synuclein. Alpha-synuclein is a major component of protein plaques in synucleinopathies, particularly Parkinson’s disease. Gallic acid (GA, 3,4,5-trihydroxy benzoic acid) is a well–known small molecule which can inhibit the formation of α-synuclein fibrils. For the process of fibrillation, purified protein was incubated at 37◦C and pH 7.2. Fibrillation was analyzed by the standard fibril methods.after that investigated fabricating of gallic acid trapped in the chitosan nanoparticles and gallic acid loaded in chitosan –coated mesoporous silica...
Design and Construction of Novel Intein Mediated Biosensor in Order to Detect Amyloid Fibrils
, M.Sc. Thesis Sharif University of Technology ; Kalhor, Hamid Reza (Supervisor)
Abstract
The most important factor in protein's function and its 3D structure is folding. Misfolding or native folding perturbation in some peptides and proteins force them get into amyloid fibril aggregation. It has been observed that these aggregations are the cause of some debilitative diseases especially ones that are related to aging like Alzheimer disease, Hantington's disease, typeII diabets etc. Recently it has been shown that specific regions in human catalase protein interact with some amyloid fibrils, such as Aß, IAPP, PrP etc. Inteins are self-splicing protein enzymes, which excise themselves from the mature protein and join the two flanking adjacent sequences by a new peptide bond in a...