Loading...
Search for:
quorum-sensing
0.008 seconds
Bacterial receiver prototype for molecular communication using rhamnose operon in a microfluidic environment
, Article IEEE Transactions on Nanobioscience ; Volume 20, Issue 4 , 2021 , Pages 426-435 ; 15361241 (ISSN) ; Mashhadian, A ; Farahnak Ghazani, M ; Arjmandi, H. R ; Alsadat Rad, M ; Shamloo, A ; Vosoughi, M ; Nasiri Kenari, M ; Sharif University of Technology
Institute of Electrical and Electronics Engineers Inc
2021
Abstract
Bacterial populations are promising candidates for the development of the receiver and transmitter nanomachines for molecular communication (MC). A bacterial receiver is required to uptake the information molecules and produce the detectable molecules following a regulation mechanism. We have constructed a novel bacterial MC receiver using an inducible bacterial L-rhamnose-regulating operon. The proposed bacterial receiver produces green fluorescent protein (GFP) in response to the L-rhamnose information molecules following a quite fast regulation mechanism. To fabricate the receiver, the bacterial population has been transformed using a plasmid harboring L-rhamnose operon genes and gene...
Design and Synthesis of Novel Coumarin Derivatives as Quorum Sensing Inhibitors & Performing Click Chemistry Involving Lysozyme Protein and a Novel Synthetic Fluorecent Compound
, M.Sc. Thesis Sharif University of Technology ; Kalhor, Hamid Reza (Supervisor)
Abstract
Quorum Sensing is a cell-to-cell communication pr°Cess depending on cell concentration. Communication among bacterial cells °Ccurs through small molecular signals called autoinducers. The consequences of quorum sensing include the activation of pathogenic properties and activation of the gene for biofilm formation. Biofilms are aggregations of bacteria that adhere to surfaces through extracellular polymeric substances. Bacteria in the biofilm phase are much more resistant to antimicrobial agents than their free-swimming counterparts (i.e. the bacteria in planktonic form). In this study, first a series of coumarin derivatives were synthesized; but due to low solubility of the synthesized...
Statistical screening of medium components for recombinant production of Pseudomonas aeruginosa ATCC 9027 rhamnolipids by nonpathogenic cell factory pseudomonas putida KT2440
, Article Molecular Biotechnology ; Vol. 56, issue. 2 , 2014 , p. 175-191 ; Jahanmiri, A ; Eslamloueyan, R ; Niazi, A ; Ayatollahi, S. S ; Aram, F ; Mahmoodi, M ; Hortamani, A ; Sharif University of Technology
Abstract
Rhamnolipids (RLs) produced by the opportunistic human pathogen Pseudomonas aeruginosa are considered as potential candidates for the next generation of surfactants. Large-scale production of RLs depends on progress in strain engineering, medium design, operating strategies, and purification procedures. In this work, the rhlAB genes extracted from a mono-RLs-producing strain of P. aeruginosa (ATCC 9027) were introduced to an appropriate safety host Pseudomonas putida KT2440. The capability of the recombinant strain was evaluated in various media. As a prerequisite for optimal medium design, a set of 32 experiments was performed in two steps for screening a number of macro-nutritional...