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rhamnolipid
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ANFIS modeling of rhamnolipid breakthrough curves on activated carbon
, Article Chemical Engineering Research and Design ; Volume 126 , 2017 , Pages 67-75 ; 02638762 (ISSN) ; Sasanipour, J ; Haratipour, P ; Alizad, M ; Vafaee Ayouri, M ; Sharif University of Technology
Institution of Chemical Engineers
2017
Abstract
Owning to interesting properties of biosurfactants such as biodegradability and lower toxicity, they have broad application in the food industry, healthy products, and bioremediation as well as for oil recovery. The present study was aimed to develop a GA-ANFIS model for predicting the breakthrough curves for rhamnolipid adsorption over activated carbon. To that end, a set of 296 adsorption data points were utilized to train the proposed FIS structure. Different graphical and statistical methods were also used to evaluate the model's accuracy and reliability. Results were then compared to those of the previously reported Artificial Neural Network (ANN) and Group Method Data Handling (GMDH)...
Development of Microorganisms with Improved Biosurfactant Activity
, Ph.D. Dissertation Sharif University of Technology ; Roosta Azad, Reza (Supervisor) ; Rouholamini Najafabadi, Abdolhossein (Supervisor) ; Akbari Noghabi, Kambiz (Co-Advisor) ; Shahcheraghi, Fereshte (Co-Advisor)
Abstract
This thesis discusses the work on the production of a rhamnolipid-type biosurfactant by Pseudomonas aeruginosa MR01, its structural characterization and environmental applications. First, results from analytical approaches for screening of biosurfactant producers suggested the oil spreading method as the fastest, simplest and most consistent analytical method. The second part of the thesis describes the properties of the most efficient biosurfactant-producing bacteria isolated from oil fields in south of Iran and according to it’s biochemical characteristics and partial sequenced 16S rRNA gene affiliated with Pseudomonas aeruginosa. Time course study indicated that the maximum biosurfactant...
Statistical screening of medium components for recombinant production of Pseudomonas aeruginosa ATCC 9027 rhamnolipids by nonpathogenic cell factory pseudomonas putida KT2440
, Article Molecular Biotechnology ; Vol. 56, issue. 2 , 2014 , p. 175-191 ; Jahanmiri, A ; Eslamloueyan, R ; Niazi, A ; Ayatollahi, S. S ; Aram, F ; Mahmoodi, M ; Hortamani, A ; Sharif University of Technology
Abstract
Rhamnolipids (RLs) produced by the opportunistic human pathogen Pseudomonas aeruginosa are considered as potential candidates for the next generation of surfactants. Large-scale production of RLs depends on progress in strain engineering, medium design, operating strategies, and purification procedures. In this work, the rhlAB genes extracted from a mono-RLs-producing strain of P. aeruginosa (ATCC 9027) were introduced to an appropriate safety host Pseudomonas putida KT2440. The capability of the recombinant strain was evaluated in various media. As a prerequisite for optimal medium design, a set of 32 experiments was performed in two steps for screening a number of macro-nutritional...
Evaluation of a recycling bioreactor for biosurfactant production by Pseudomonas aeruginosa MR01 using soybean oil waste
, Article Journal of Chemical Technology and Biotechnology ; Volume 91, Issue 5 , 2016 , Pages 1368-1377 ; 02682575 (ISSN) ; Ebadipour, N ; Roostaazad, R ; Sharif University of Technology
John Wiley and Sons Ltd
Abstract
This study deals with the large-scale production of biosurfactant from soybean oil soapstock by Pseudomonas aeruginosa MR01. The production of biosurfactant was carried out in a newly designed bioreactor equipped with recycling flow under three operational conditions. Kinetic studies were conducted at both shake flask and 5-L bioreactor scales during fermentation in a soapstock medium. Mathematical equations were developed to model the kinetic patterns at both scales. RESULT: Statistical analyses demonstrated the goodness of fit, with regression r-squared, R2, between 0.97and 0.99 for different models. Furthermore, biosurfactant concentration in the bioreactor including the recycling flow,...
Two schemes for production of biosurfactant from Pseudomonas aeruginosa MR01: Applying residues from soybean oil industry and silica sol–gel immobilized cells
, Article Colloids and Surfaces B: Biointerfaces ; Volume 152 , 2017 , Pages 159-168 ; 09277765 (ISSN) ; Ebadipour, N ; Roostaazad, R ; Partovi, M ; Bahmaei, M ; Sharif University of Technology
Elsevier B.V
2017
Abstract
Rhamnolipids are the most common biosurfactants and P. aeruginosa strains are the most frequently studied microorganisms for the production of rhamnolipids. Eco-friendly advantages and promising applications of rhamnolipids in various industries are the major reasons for pursuing the economic production of these biosurfactants. This study shows that cultivation of P. aeruginosa MR01 in medium contained inexpensive soybean oil refinery wastes which exhibited similar levels and homologues of rhamnolipids. Mass spectrometry indicated that the Rha-C10-C10 and Rha-Rha-C10-C10 constitute the main rhamnolipids in different cultures of MR01 including one of oil carbon source analogues. Moreover,...
Performance Evaluation of Rhamnolipid & Sophorolipid Biosurfactants Capable of Wettability Change and Oil Recovery Increase
, M.Sc. Thesis Sharif University of Technology ; Taghikhani, Vahid (Supervisor) ; Baghban Salehi, Mahsa (Supervisor) ; Mokhtarani, Babak (Co-Supervisor)
Abstract
As fossil fuels are still the world's most important source of energy, as well as the maturity of most of the world's oil reservoirs, there is a need for enhanced oil recovery methods to meet the world's increasing oil demands. MEOR methods are new and promising methods that are still in the research and development stage. One of the MEOR methods is the use of biosurfactants, which increase the oil recovery by two mechanisms: 1) reducing the interfacial tension between water and oil and 2) changing the wettability of the rock. In this study, the performance of two glycolipid biosurfactants, rhamnolipid and sophorolipid, in increasing oil recovery has been investigated. Initially, surface...
Management of soybean oil refinery wastes through recycling them for producing biosurfactant using Pseudomonas aeruginosa MR01
, Article World Journal of Microbiology and Biotechnology ; Volume 29, Issue 6 , June , 2013 , Pages 1039-1047 ; 09593993 (ISSN) ; Lotfabad, T. B ; Roostaazad, R ; Bahmaei, M ; Tayyebi, S ; Sharif University of Technology
2013
Abstract
Biosurfactant production through a fermentation process involving the biodegradation of soybean oil refining wastes was studied. Pseudomonas aeruginosa MR01 was able to produce extracellular biosurfactant when it was cultured in three soybean oil refinement wastes; acid oil, deodorizer distillate and soapstock, at different carbon to nitrogen ratios. Subsequent fermentation kinetics in the three types of waste culture were also investigated and compared with kinetic behavior in soybean oil medium. Biodegradation of wastes, biosurfactant production, biomass growth, nitrate consumption and the number of colony forming units were detected in four proposed media, at specified time intervals....
Structural characterization of a rhamnolipid-type biosurfactant produced by Pseudomonas aeruginosa MR01: Enhancement of di-rhamnolipid proportion using gamma irradiation
, Article Colloids and Surfaces B: Biointerfaces ; Volume 81, Issue 2 , 2010 , Pages 397-405 ; 09277765 (ISSN) ; Abassi, H ; Ahmadkhaniha, R ; Roostaazad, R ; Masoomi, F ; Zahiri, H. S ; Ahmadian, G ; Vali, H ; Noghabi, K. A ; Sharif University of Technology
2010
Abstract
We previously reported that MR01, an indigenous strain of Pseudomonas aeruginosa, was able to produce a rhamnolipid-type biosurfactant. Here, we attempted to define the structural properties of this natural product. The analysis of the extracted biosurfactant by thin-layer chromatography (TLC) revealed the presence of two compounds corresponding to those of authentic mono- and di-rhamnolipid. The identity of two structurally distinguished rhamnolipids was confirmed by 1H and 13C nuclear magnetic resonance (NMR) spectroscopy. Liquid chromatography/mass spectrometry (LC/MS) of extracted biosurfactant revealed up to seventeen different rhamnolipid congeners. Further quantification showed...